Values CatLog read from this paper
Structure and function of phosphonoacetaldehyde dehydrogenase: the missing link in phosphonoacetate formation
PMID 24361046doi:10.1016/j.chembiol.2013.11.006Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 19 records (29 values) from this paper: PhnY (phosphonoacetaldehyde dehydrogenase), GAPDH (non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase) from Sinorhizobium meliloti, Streptococcus mutans.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| GAPDH (non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase) | Streptococcus mutans | 3-oxopropyl phosphonate | 0.038 | 1.5 | 0.025 calc. | pH 8, 30 °C | Open record: GAPDH (non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase), 3-oxopropyl phosphonate |
| GAPDH (non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase) | Streptococcus mutans | DL-glyceraldehyde 3-phosphate | 22 | 0.025 | 880 calc. | pH 8, 30 °C | Open record: GAPDH (non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase), DL-glyceraldehyde 3-phosphate |
| GAPDH (non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase) | Streptococcus mutans | NADP+cosubstrate 3-OPP | — | 0.33 | — | pH 8, 30 °C | Open record: GAPDH (non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase), NADP+ |
| GAPDH (non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase) | Streptococcus mutans | NADP+cosubstrate G3P (racemic) | — | 0.022 | — | pH 8, 30 °C | Open record: GAPDH (non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase), NADP+ |
| PhnY (phosphonoacetaldehyde dehydrogenase) | Sinorhizobium meliloti | 3-oxopropyl phosphonate | 1.5 | 3.3 | 0.45 calc. | pH 7.5, 30 °C | Open record: PhnY (phosphonoacetaldehyde dehydrogenase), 3-oxopropyl phosphonate |
| PhnY (phosphonoacetaldehyde dehydrogenase) | Sinorhizobium meliloti | glyceraldehyde-3-phosphate | 0.098 | 0.097 | 1.0 calc. | pH 7.5, 30 °C | Open record: PhnY (phosphonoacetaldehyde dehydrogenase), glyceraldehyde-3-phosphate |
| PhnY (phosphonoacetaldehyde dehydrogenase) | Sinorhizobium meliloti | NAD+cosubstrate PnAA | — | 0.058 | — | pH 7.5, 30 °C | Open record: PhnY (phosphonoacetaldehyde dehydrogenase), NAD+ |
| PhnY (phosphonoacetaldehyde dehydrogenase) | Sinorhizobium meliloti | NAD+cosubstrate G3P | — | 0.53 | — | pH 7.5, 30 °C | Open record: PhnY (phosphonoacetaldehyde dehydrogenase), NAD+ |
| PhnY (phosphonoacetaldehyde dehydrogenase) | Sinorhizobium meliloti | phosphonoacetaldehyde | 2.2 | 0.0032 | 690 calc. | pH 7.5, 30 °C | Open record: PhnY (phosphonoacetaldehyde dehydrogenase), phosphonoacetaldehyde |
| PhnY (phosphonoacetaldehyde dehydrogenase)variant E385A | Sinorhizobium meliloti | NAD+ | — | 0.37 | — | pH 7.5, 30 °C | Open record: PhnY (phosphonoacetaldehyde dehydrogenase) variant E385A, NAD+ |
| PhnY (phosphonoacetaldehyde dehydrogenase)variant E385A | Sinorhizobium meliloti | phosphonoacetaldehyde | 0.19 | 0.019 | 10 calc. | pH 7.5, 30 °C | Open record: PhnY (phosphonoacetaldehyde dehydrogenase) variant E385A, phosphonoacetaldehyde |
| PhnY (phosphonoacetaldehyde dehydrogenase)variant N158A | Sinorhizobium meliloti | NAD+ | — | 0.04 | — | pH 7.5, 30 °C | Open record: PhnY (phosphonoacetaldehyde dehydrogenase) variant N158A, NAD+ |
| PhnY (phosphonoacetaldehyde dehydrogenase)variant N158A | Sinorhizobium meliloti | phosphonoacetaldehyde | 0.01 | 0.029 | 0.34 calc. | pH 7.5, 30 °C | Open record: PhnY (phosphonoacetaldehyde dehydrogenase) variant N158A, phosphonoacetaldehyde |
| PhnY (phosphonoacetaldehyde dehydrogenase)variant R108A | Sinorhizobium meliloti | NAD+ | — | 0.018 | — | pH 7.5, 30 °C | Open record: PhnY (phosphonoacetaldehyde dehydrogenase) variant R108A, NAD+ |
| PhnY (phosphonoacetaldehyde dehydrogenase)variant R108A | Sinorhizobium meliloti | phosphonoacetaldehyde | 0.051 | 0.0097 | 5.3 calc. | pH 7.5, 30 °C | Open record: PhnY (phosphonoacetaldehyde dehydrogenase) variant R108A, phosphonoacetaldehyde |
| PhnY (phosphonoacetaldehyde dehydrogenase)variant R290A | Sinorhizobium meliloti | NAD+ | — | 0.075 | — | pH 7.5, 30 °C | Open record: PhnY (phosphonoacetaldehyde dehydrogenase) variant R290A, NAD+ |
| PhnY (phosphonoacetaldehyde dehydrogenase)variant R290A | Sinorhizobium meliloti | phosphonoacetaldehyde | 0.12 | 0.0051 | 24 calc. | pH 7.5, 30 °C | Open record: PhnY (phosphonoacetaldehyde dehydrogenase) variant R290A, phosphonoacetaldehyde |
| PhnY (phosphonoacetaldehyde dehydrogenase)variant R447A | Sinorhizobium meliloti | NAD+ | — | 0.054 | — | pH 7.5, 30 °C | Open record: PhnY (phosphonoacetaldehyde dehydrogenase) variant R447A, NAD+ |
| PhnY (phosphonoacetaldehyde dehydrogenase)variant R447A | Sinorhizobium meliloti | phosphonoacetaldehyde | 0.076 | 0.15 | 0.51 calc. | pH 7.5, 30 °C | Open record: PhnY (phosphonoacetaldehyde dehydrogenase) variant R447A, phosphonoacetaldehyde |