Values CatLog read from this paper
Engineering acidic Streptomyces rubiginosus D-xylose isomerase by rational enzyme design
PMID 24402330doi:10.1093/protein/gzt062Open in Browse
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CatLog read 21 records (63 values) from this paper: D-xylose isomerase from Streptomyces rubiginosus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-xylose isomerase | Streptomyces rubiginosus | D-xylosenative enzyme; commercial (Hampton Research) | 5.52 | 3 | 1.84 | pH 7.7, 25 °C | Open record: D-xylose isomerase, D-xylose |
| D-xylose isomerase | Streptomyces rubiginosus | D-xylosenative enzyme; commercial (Hampton Research) | 0.41 | 83 | 0.0049 | pH 5.8, 25 °C | Open record: D-xylose isomerase, D-xylose |
| D-xylose isomerase | Streptomyces rubiginosus | D-xyloserecombinant; N-terminal His6 tag | 1.22 | 5 | 0.24 | pH 7.7, 25 °C | Open record: D-xylose isomerase, D-xylose |
| D-xylose isomerase | Streptomyces rubiginosus | D-xyloserecombinant; N-terminal His6 tag | 0.31 | 33 | 0.0094 | pH 5.8, 25 °C | Open record: D-xylose isomerase, D-xylose |
| D-xylose isomerase | Streptomyces rubiginosus | D-xyloserecombinant; N-terminal His12 tag | 0.8 | 3.8 | 0.211 | pH 7.7, 25 °C | Open record: D-xylose isomerase, D-xylose |
| D-xylose isomerase | Streptomyces rubiginosus | D-xyloserecombinant; N-terminal His12 tag | 0.14 | 14 | 0.01 | pH 5.8, 25 °C | Open record: D-xylose isomerase, D-xylose |
| D-xylose isomerasevariant D57H | Streptomyces rubiginosus | D-xylose | 0.18 | 7.4 | 0.024 | pH 7.7, 25 °C | Open record: D-xylose isomerase variant D57H, D-xylose |
| D-xylose isomerasevariant D57H | Streptomyces rubiginosus | D-xylose | 0.07 | 548 | 1.3 × 10⁻⁴ | pH 5.8, 25 °C | Open record: D-xylose isomerase variant D57H, D-xylose |
| D-xylose isomerasevariant D57N | Streptomyces rubiginosus | D-xylose | 0.96 | 5.3 | 0.181 | pH 7.7, 25 °C | Open record: D-xylose isomerase variant D57N, D-xylose |
| D-xylose isomerasevariant D57N | Streptomyces rubiginosus | D-xylose | 0.33 | 45 | 0.007 | pH 5.8, 25 °C | Open record: D-xylose isomerase variant D57N, D-xylose |
| D-xylose isomerasevariant E186Q/N215D | Streptomyces rubiginosus | D-xylose | 0.66 | 11.2 | 0.059 | pH 7.7, 25 °C | Open record: D-xylose isomerase variant E186Q/N215D, D-xylose |
| D-xylose isomerasevariant E186Q/N215D | Streptomyces rubiginosus | D-xylose | 0.056 | 15 | 0.0037 | pH 5.8, 25 °C | Open record: D-xylose isomerase variant E186Q/N215D, D-xylose |
| D-xylose isomerasevariant K289E | Streptomyces rubiginosus | D-xylose | 0.51 | 8 | 0.064 | pH 7.7, 25 °C | Open record: D-xylose isomerase variant K289E, D-xylose |
| D-xylose isomerasevariant K289E | Streptomyces rubiginosus | D-xylose | 0.07 | 37 | 0.0019 | pH 5.8, 25 °C | Open record: D-xylose isomerase variant K289E, D-xylose |
| D-xylose isomerasevariant K289H | Streptomyces rubiginosus | D-xylose | 1.38 | 4.3 | 0.321 | pH 7.7, 25 °C | Open record: D-xylose isomerase variant K289H, D-xylose |
| D-xylose isomerasevariant K289H | Streptomyces rubiginosus | D-xylose | 0.064 | 27 | 0.0024 | pH 5.8, 25 °C | Open record: D-xylose isomerase variant K289H, D-xylose |
| D-xylose isomerasevariant N215D | Streptomyces rubiginosus | D-xylose | 2.3 | 3.4 | 0.676 | pH 7.7, 25 °C | Open record: D-xylose isomerase variant N215D, D-xylose |
| D-xylose isomerasevariant N215D | Streptomyces rubiginosus | D-xylose | 0.19 | 10 | 0.019 | pH 5.8, 25 °C | Open record: D-xylose isomerase variant N215D, D-xylose |
| D-xylose isomerasevariant N247D | Streptomyces rubiginosus | D-xylose | 0.11 | 6 | 0.018 | pH 7.7, 25 °C | Open record: D-xylose isomerase variant N247D, D-xylose |
| D-xylose isomerasevariant N247D | Streptomyces rubiginosus | D-xylose | 0.074 | 42 | 0.0018 | pH 5.8, 25 °C | Open record: D-xylose isomerase variant N247D, D-xylose |
| D-xylose isomerasevariant Q256D | Streptomyces rubiginosus | D-xylose | 0.15 | 6.5 | 0.023 | pH 7.7, 25 °C | Open record: D-xylose isomerase variant Q256D, D-xylose |