Values CatLog read from this paper
Crystal structure and biochemical studies of Brucella melitensis 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
PMID 24657441doi:10.1016/j.bbrc.2014.03.045Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 26 records (78 values) from this paper: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) from Brucella melitensis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) | Brucella melitensis | 5'-methylthioadenosine | 8.8 | 0.0022 | 4 × 10³ | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN), 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) | Brucella melitensis | S-adenosylhomocysteine | 7.1 | 0.004 | 1.8 × 10³ | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN), S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant A15M | Brucella melitensis | 5'-methylthioadenosine | 1.8 | 0.0031 | 580 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant A15M, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant A15M | Brucella melitensis | S-adenosylhomocysteine | 1.1 | 0.0041 | 270 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant A15M, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant M144A | Brucella melitensis | 5'-methylthioadenosine | 0.51 | 0.0351 | 15 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant M144A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant M144A | Brucella melitensis | S-adenosylhomocysteine | 0.15 | 0.0951 | 1.6 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant M144A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant R164A | Brucella melitensis | 5'-methylthioadenosine | 8 | 0.008 | 600 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant R164A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant R164A | Brucella melitensis | S-adenosylhomocysteine | 4.7 | 0.0133 | 350 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant R164A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant R85A | Brucella melitensis | 5'-methylthioadenosine | 1.4 | 0.0054 | 250 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant R85A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant R85A | Brucella melitensis | S-adenosylhomocysteine | 0.93 | 0.0068 | 140 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant R85A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant R85H | Brucella melitensis | 5'-methylthioadenosine | 1.3 | 0.0048 | 280 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant R85H, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant R85H | Brucella melitensis | S-adenosylhomocysteine | 0.92 | 0.0061 | 150 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant R85H, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant S167A | Brucella melitensis | 5'-methylthioadenosine | 0.58 | 0.0044 | 130 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant S167A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant S167A | Brucella melitensis | S-adenosylhomocysteine | 0.55 | 0.0056 | 100 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant S167A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant V34A | Brucella melitensis | 5'-methylthioadenosine | 2.7 | 0.0053 | 510 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant V34A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant V34A | Brucella melitensis | S-adenosylhomocysteine | 2.1 | 0.0062 | 330 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant V34A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant V34I | Brucella melitensis | 5'-methylthioadenosine | 2.5 | 0.0045 | 560 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant V34I, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant V34I | Brucella melitensis | S-adenosylhomocysteine | 2.3 | 0.0058 | 390 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant V34I, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant W179A | Brucella melitensis | 5'-methylthioadenosine | 0.69 | 0.0054 | 130 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant W179A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant W179A | Brucella melitensis | S-adenosylhomocysteine | 0.86 | 0.0076 | 110 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant W179A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant W179F | Brucella melitensis | 5'-methylthioadenosine | 0.58 | 0.0074 | 79 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant W179F, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant W179F | Brucella melitensis | S-adenosylhomocysteine | 0.37 | 0.0098 | 38 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant W179F, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant Y134A | Brucella melitensis | 5'-methylthioadenosine | 0.78 | 0.028 | 28 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant Y134A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant Y134A | Brucella melitensis | S-adenosylhomocysteine | 0.39 | 0.0381 | 10 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant Y134A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant Y134F | Brucella melitensis | 5'-methylthioadenosine | 0.71 | 0.0301 | 24 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant Y134F, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN)variant Y134F | Brucella melitensis | S-adenosylhomocysteine | 0.35 | 0.0452 | 7.8 | pH 7.5, 25 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (BmMTAN) variant Y134F, S-adenosylhomocysteine |