Values CatLog read from this paper
Cloning, expression, and directed evolution of carbonyl reductase from Leifsonia xyli HS0904 with enhanced catalytic efficiency
PMID 24788330doi:10.1007/s00253-014-5770-zOpen in Browse
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CatLog read 6 records (18 values) from this paper: carbonyl reductase (LXCAR) from Leifsonia xyli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| carbonyl reductase (LXCAR) | Leifsonia xyli | 3,5-bis(trifluoromethyl)acetophenone | 21.1 | 0.573 | 36.8 | pH 7.2, 30 °C | Open record: carbonyl reductase (LXCAR), 3,5-bis(trifluoromethyl)acetophenone |
| carbonyl reductase (LXCAR) | Leifsonia xyli | NADH | 23.6 | 0.025 | 944 | pH 7.2, 30 °C | Open record: carbonyl reductase (LXCAR), NADH |
| carbonyl reductase (LXCAR)variant S154Y | Leifsonia xyli | 3,5-bis(trifluoromethyl)acetophenone | 114 | 0.201 | 463 | pH 7.2, 30 °C | Open record: carbonyl reductase (LXCAR) variant S154Y, 3,5-bis(trifluoromethyl)acetophenone |
| carbonyl reductase (LXCAR)variant S154Y | Leifsonia xyli | NADH | 122 | 0.029 | 4207 | pH 7.2, 30 °C | Open record: carbonyl reductase (LXCAR) variant S154Y, NADH |
| carbonyl reductase (LXCAR)variant S154Y/L194I | Leifsonia xyli | 3,5-bis(trifluoromethyl)acetophenone | 89.2 | 0.323 | 277 | pH 7.2, 30 °C | Open record: carbonyl reductase (LXCAR) variant S154Y/L194I, 3,5-bis(trifluoromethyl)acetophenone |
| carbonyl reductase (LXCAR)variant S154Y/L194I | Leifsonia xyli | NADH | 100 | 0.026 | 3846 | pH 7.2, 30 °C | Open record: carbonyl reductase (LXCAR) variant S154Y/L194I, NADH |