Values CatLog read from this paper
First functional and mutational analysis of group 3 N-acetylneuraminate lyases from Lactobacillus antri and Lactobacillus sakei 23K
PMID 24817128doi:10.1371/journal.pone.0096976Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (39 values) from this paper: N-acetylneuraminate lyase (LaNAL), N-acetylneuraminate lyase (LsNAL) from Lactobacillus antri, Lactobacillus sakei.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| N-acetylneuraminate lyase (LaNAL) | Lactobacillus antri | N-acetyl-D-mannosamine | 5.4 | 333 | 0.016 | pH 7, 37 °C | Open record: N-acetylneuraminate lyase (LaNAL), N-acetyl-D-mannosamine |
| N-acetylneuraminate lyase (LaNAL) | Lactobacillus antri | N-acetylneuraminic acid | 1.9 | 1.1 | 1.7 | pH 7, 37 °C | Open record: N-acetylneuraminate lyase (LaNAL), N-acetylneuraminic acid |
| N-acetylneuraminate lyase (LaNAL) | Lactobacillus antri | pyruvate | 5.6 | 1.1 | 5.1 | pH 7, 37 °C | Open record: N-acetylneuraminate lyase (LaNAL), pyruvate |
| N-acetylneuraminate lyase (LaNAL)variant G210S/Y213G | Lactobacillus antri | N-acetyl-D-mannosamine | 15.3 | 3.2 × 10³ | 0.004 | pH 7, 37 °C | Open record: N-acetylneuraminate lyase (LaNAL) variant G210S/Y213G, N-acetyl-D-mannosamine |
| N-acetylneuraminate lyase (LaNAL)variant G210S/Y213G | Lactobacillus antri | N-acetylneuraminic acid | 0.47 | 2 | 0.23 | pH 7, 37 °C | Open record: N-acetylneuraminate lyase (LaNAL) variant G210S/Y213G, N-acetylneuraminic acid |
| N-acetylneuraminate lyase (LaNAL)variant G210S/Y213G | Lactobacillus antri | pyruvate | 3 | 4.3 | 0.71 | pH 7, 37 °C | Open record: N-acetylneuraminate lyase (LaNAL) variant G210S/Y213G, pyruvate |
| N-acetylneuraminate lyase (LaNAL)variant G211S | Lactobacillus antri | N-acetylneuraminic acid | 0.032 | 1.2 | 0.026 | pH 7, 37 °C | Open record: N-acetylneuraminate lyase (LaNAL) variant G211S, N-acetylneuraminic acid |
| N-acetylneuraminate lyase (LaNAL)variant P193Y | Lactobacillus antri | N-acetyl-D-mannosamine | 2.4 | 915 | 0.002 | pH 7, 37 °C | Open record: N-acetylneuraminate lyase (LaNAL) variant P193Y, N-acetyl-D-mannosamine |
| N-acetylneuraminate lyase (LaNAL)variant P193Y | Lactobacillus antri | N-acetylneuraminic acid | 0.13 | 0.27 | 0.48 | pH 7, 37 °C | Open record: N-acetylneuraminate lyase (LaNAL) variant P193Y, N-acetylneuraminic acid |
| N-acetylneuraminate lyase (LaNAL)variant P193Y | Lactobacillus antri | pyruvate | 0.99 | 0.5 | 1.98 | pH 7, 37 °C | Open record: N-acetylneuraminate lyase (LaNAL) variant P193Y, pyruvate |
| N-acetylneuraminate lyase (LsNAL) | Lactobacillus sakei | N-acetyl-D-mannosamine | 4.6 | 272 | 0.016 | pH 7, 37 °C | Open record: N-acetylneuraminate lyase (LsNAL), N-acetyl-D-mannosamine |
| N-acetylneuraminate lyase (LsNAL) | Lactobacillus sakei | N-acetylneuraminic acid | 0.9 | 0.32 | 2.8 | pH 7, 37 °C | Open record: N-acetylneuraminate lyase (LsNAL), N-acetylneuraminic acid |
| N-acetylneuraminate lyase (LsNAL) | Lactobacillus sakei | pyruvate | 3.9 | 12.5 | 0.31 | pH 7, 37 °C | Open record: N-acetylneuraminate lyase (LsNAL), pyruvate |