Values CatLog read from this paper
Structural and mutational analysis of amino acid residues involved in ATP specificity of Escherichia coli acetate kinase
PMID 24856051doi:10.1016/j.jbiosc.2014.04.014Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (16 values) from this paper: acetate kinase (ATP-ecoAK), pyrophosphate-dependent acetate kinase (PPi-ehiAK) from Escherichia coli, Entamoeba histolytica.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| acetate kinase (ATP-ecoAK) | Escherichia coli | adenosine 5'-triphosphate | 908 | 0.67 | 1.4 × 10³ calc. | pH 6.5, 30 °C | Open record: acetate kinase (ATP-ecoAK), adenosine 5'-triphosphate |
| acetate kinase (ATP-ecoAK)variant G332D | Escherichia coli | adenosine 5'-triphosphate | 9.9 | 3.5 | 2.8 calc. | pH 6.5, 30 °C | Open record: acetate kinase (ATP-ecoAK) variant G332D, adenosine 5'-triphosphate |
| acetate kinase (ATP-ecoAK)variant G333Q | Escherichia coli | adenosine 5'-triphosphate | 41 | 5.2 | 7.9 calc. | pH 6.5, 30 °C | Open record: acetate kinase (ATP-ecoAK) variant G333Q, adenosine 5'-triphosphate |
| acetate kinase (ATP-ecoAK)variant I334M | Escherichia coli | adenosine 5'-triphosphate | 10.5 | 6.6 | 1.6 calc. | pH 6.5, 30 °C | Open record: acetate kinase (ATP-ecoAK) variant I334M, adenosine 5'-triphosphate |
| acetate kinase (ATP-ecoAK)variant N213T | Escherichia coli | adenosine 5'-triphosphate | 16.3 | 1.7 | 9.6 calc. | pH 6.5, 30 °C | Open record: acetate kinase (ATP-ecoAK) variant N213T, adenosine 5'-triphosphate |
| acetate kinase (ATP-ecoAK)variant N213T/G332D/E336L/T385N | Escherichia coli | adenosine 5'-triphosphate | 0.34 | 0.66 | 0.52 calc. | pH 6.5, 30 °C | Open record: acetate kinase (ATP-ecoAK) variant N213T/G332D/E336L/T385N, adenosine 5'-triphosphate |
| acetate kinase (ATP-ecoAK)variant N337E | Escherichia coli | adenosine 5'-triphosphate | 395 | 30.7 | 12.9 calc. | pH 6.5, 30 °C | Open record: acetate kinase (ATP-ecoAK) variant N337E, adenosine 5'-triphosphate |
| pyrophosphate-dependent acetate kinase (PPi-ehiAK) | Entamoeba histolytica | pyrophosphate | 2.6 | 3.3 | 0.79 calc. | pH 6.5, 30 °C | Open record: pyrophosphate-dependent acetate kinase (PPi-ehiAK), pyrophosphate |