Values CatLog read from this paper
Increasing the reaction rate of hydroxynitrile lyase from Hevea brasiliensis toward mandelonitrile by copying active site residues from an esterase that accepts aromatic esters
PMID 25044660doi:10.1002/cbic.201402081Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 38 records (102 values) from this paper: hydroxynitrile lyase (HbHNL) from Hevea brasiliensis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| hydroxynitrile lyase (HbHNL) | Hevea brasiliensis | racemic 2-nitro-1-phenylethanol | 0.15 | 6.1 | 0.025 calc. | pH 5 | Open record: hydroxynitrile lyase (HbHNL), racemic 2-nitro-1-phenylethanol |
| hydroxynitrile lyase (HbHNL) | Hevea brasiliensis | racemic mandelonitrile | 25 | 3.3 | 7.4 | pH 5 | Open record: hydroxynitrile lyase (HbHNL), racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant C81L | Hevea brasiliensis | racemic mandelonitrile | 33 | 9.4 | 3.5 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant C81L, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant F125T | Hevea brasiliensis | racemic 2-nitro-1-phenylethanol | 0.6 | — | — | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant F125T, racemic 2-nitro-1-phenylethanol |
| hydroxynitrile lyase (HbHNL)variant F125T | Hevea brasiliensis | racemic mandelonitrile | 66 | 2 | 33 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant F125T, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant F125T/L146M | Hevea brasiliensis | racemic 2-nitro-1-phenylethanol | 0.1 | 0.4 | 0.25 calc. | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant F125T/L146M, racemic 2-nitro-1-phenylethanol |
| hydroxynitrile lyase (HbHNL)variant F125T/L146M | Hevea brasiliensis | racemic mandelonitrile | 32 | 1.5 | 21 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant F125T/L146M, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant F125T/Y133F | Hevea brasiliensis | racemic 2-nitro-1-phenylethanol | 0.12 | 0.7 | 0.17 calc. | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant F125T/Y133F, racemic 2-nitro-1-phenylethanol |
| hydroxynitrile lyase (HbHNL)variant F125T/Y133F | Hevea brasiliensis | racemic mandelonitrile | 32 | 2.1 | 15 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant F125T/Y133F, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant F210I | Hevea brasiliensis | racemic mandelonitrile | 21 | 8.6 | 2.5 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant F210I, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant I209A | Hevea brasiliensis | racemic mandelonitrile | 23 | 0.8 | 29 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant I209A, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant I209G | Hevea brasiliensis | racemic mandelonitrile | 3 | 0.7 | 4 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant I209G, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L121A | Hevea brasiliensis | racemic mandelonitrile | 23 | 2.8 | 8.3 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L121A, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L121C | Hevea brasiliensis | racemic mandelonitrile | 20 | 8.1 | 2.5 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L121C, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L121F | Hevea brasiliensis | racemic mandelonitrile | 77 | 1.5 | 51 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L121F, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L121H | Hevea brasiliensis | racemic mandelonitrile | 5 | 8 | 0.6 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L121H, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L121I | Hevea brasiliensis | racemic mandelonitrile | 33 | 4.9 | 6.6 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L121I, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L121M | Hevea brasiliensis | racemic mandelonitrile | 43 | 4 | 11 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L121M, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L121Q | Hevea brasiliensis | racemic mandelonitrile | 6 | 5.7 | 1.1 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L121Q, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L121S | Hevea brasiliensis | racemic mandelonitrile | 9 | 6.6 | 1.4 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L121S, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L121T | Hevea brasiliensis | racemic mandelonitrile | 3 | 2.7 | 1.4 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L121T, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L121V | Hevea brasiliensis | racemic mandelonitrile | 15 | 3.7 | 4 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L121V, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L121W | Hevea brasiliensis | racemic mandelonitrile | 15 | 5.6 | 2.6 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L121W, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L121Y | Hevea brasiliensis | racemic mandelonitrile | 106 | 2.7 | 39 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L121Y, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L121Y/F125T | Hevea brasiliensis | racemic mandelonitrile | 43 | 3.1 | 14 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L121Y/F125T, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L121Y/F125T/L146M | Hevea brasiliensis | racemic mandelonitrile | 45 | 3.6 | 12 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L121Y/F125T/L146M, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L121Y/L146M | Hevea brasiliensis | racemic mandelonitrile | — | 6.7 | — | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L121Y/L146M, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L146M | Hevea brasiliensis | racemic mandelonitrile | 20 | 3.7 | 5.4 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L146M, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L148F | Hevea brasiliensis | racemic mandelonitrile | 17 | 8.6 | 1 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L148F, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant L152F | Hevea brasiliensis | racemic mandelonitrile | 7 | 0.6 | 11 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant L152F, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant V106F | Hevea brasiliensis | racemic 2-nitro-1-phenylethanol | 0.1 | 1.4 | 0.071 calc. | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant V106F, racemic 2-nitro-1-phenylethanol |
| hydroxynitrile lyase (HbHNL)variant V106F | Hevea brasiliensis | racemic mandelonitrile | 7 | 5.3 | 1.4 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant V106F, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant V106F/F125T | Hevea brasiliensis | racemic 2-nitro-1-phenylethanol | 0.2 | 1.8 | 0.11 calc. | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant V106F/F125T, racemic 2-nitro-1-phenylethanol |
| hydroxynitrile lyase (HbHNL)variant V106F/L121Y | Hevea brasiliensis | racemic 2-nitro-1-phenylethanol | — | 1.6 | — | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant V106F/L121Y, racemic 2-nitro-1-phenylethanol |
| hydroxynitrile lyase (HbHNL)variant V106F/L121Y | Hevea brasiliensis | racemic mandelonitrile | 55 | 1.4 | 39 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant V106F/L121Y, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant V106F/L121Y/F125T | Hevea brasiliensis | racemic mandelonitrile | 82 | 2 | 41 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant V106F/L121Y/F125T, racemic mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant Y133F | Hevea brasiliensis | racemic 2-nitro-1-phenylethanol | 0.3 | 7.1 | 0.042 calc. | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant Y133F, racemic 2-nitro-1-phenylethanol |
| hydroxynitrile lyase (HbHNL)variant Y133F | Hevea brasiliensis | racemic mandelonitrile | 22 | 3.1 | 7.1 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant Y133F, racemic mandelonitrile |