Values CatLog read from this paper
Identification and characterization of a tyramine-glutamate ligase (MfnD) involved in methanofuran biosynthesis
PMID 25211225doi:10.1021/bi500879hOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (33 values) from this paper: MfnD (tyramine-glutamate ligase, Mefer_1180 gene product) from Methanocaldococcus fervens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| MfnD (tyramine-glutamate ligase, Mefer_1180 gene product) | Methanocaldococcus fervens | 4-(2-aminoethyl)phenol | 4.8 | 0.8 | 6 | pH 6.7, 70 °C | Open record: MfnD (tyramine-glutamate ligase, Mefer_1180 gene product), 4-(2-aminoethyl)phenol |
| MfnD (tyramine-glutamate ligase, Mefer_1180 gene product) | Methanocaldococcus fervens | adenosine 5'-triphosphate | 5.9 | 1.5 | 3.9 | pH 6.7, 70 °C | Open record: MfnD (tyramine-glutamate ligase, Mefer_1180 gene product), adenosine 5'-triphosphate |
| MfnD (tyramine-glutamate ligase, Mefer_1180 gene product) | Methanocaldococcus fervens | L-glutamate | 5.8 | 2.3 | 2.5 | pH 6.7, 70 °C | Open record: MfnD (tyramine-glutamate ligase, Mefer_1180 gene product), L-glutamate |
| MfnD (tyramine-glutamate ligase, Mefer_1180 gene product)variant E21Q | Methanocaldococcus fervens | 4-(2-aminoethyl)phenol | 1.7 | 1.1 | 1.7 | pH 6.7, 70 °C | Open record: MfnD (tyramine-glutamate ligase, Mefer_1180 gene product) variant E21Q, 4-(2-aminoethyl)phenol |
| MfnD (tyramine-glutamate ligase, Mefer_1180 gene product)variant E21Q | Methanocaldococcus fervens | adenosine 5'-triphosphate | 2.1 | 2 | 1.1 | pH 6.7, 70 °C | Open record: MfnD (tyramine-glutamate ligase, Mefer_1180 gene product) variant E21Q, adenosine 5'-triphosphate |
| MfnD (tyramine-glutamate ligase, Mefer_1180 gene product)variant E21Q | Methanocaldococcus fervens | L-glutamate | 2.7 | 7.3 | 0.37 | pH 6.7, 70 °C | Open record: MfnD (tyramine-glutamate ligase, Mefer_1180 gene product) variant E21Q, L-glutamate |
| MfnD (tyramine-glutamate ligase, Mefer_1180 gene product)variant R251A | Methanocaldococcus fervens | 4-(2-aminoethyl)phenol | 0.05 | 0.8 | 0.06 | pH 6.7, 70 °C | Open record: MfnD (tyramine-glutamate ligase, Mefer_1180 gene product) variant R251A, 4-(2-aminoethyl)phenol |
| MfnD (tyramine-glutamate ligase, Mefer_1180 gene product)variant R251A | Methanocaldococcus fervens | adenosine 5'-triphosphate | 0.07 | 3 | 0.023 | pH 6.7, 70 °C | Open record: MfnD (tyramine-glutamate ligase, Mefer_1180 gene product) variant R251A, adenosine 5'-triphosphate |
| MfnD (tyramine-glutamate ligase, Mefer_1180 gene product)variant T253V | Methanocaldococcus fervens | 4-(2-aminoethyl)phenol | 1.4 | 0.6 | 2.3 | pH 6.7, 70 °C | Open record: MfnD (tyramine-glutamate ligase, Mefer_1180 gene product) variant T253V, 4-(2-aminoethyl)phenol |
| MfnD (tyramine-glutamate ligase, Mefer_1180 gene product)variant T253V | Methanocaldococcus fervens | adenosine 5'-triphosphate | 1.4 | 2.5 | 0.56 | pH 6.7, 70 °C | Open record: MfnD (tyramine-glutamate ligase, Mefer_1180 gene product) variant T253V, adenosine 5'-triphosphate |
| MfnD (tyramine-glutamate ligase, Mefer_1180 gene product)variant T253V | Methanocaldococcus fervens | L-glutamate | 1.3 | 3.6 | 0.36 | pH 6.7, 70 °C | Open record: MfnD (tyramine-glutamate ligase, Mefer_1180 gene product) variant T253V, L-glutamate |