Values CatLog read from this paper
Picosecond-resolved fluorescence studies of substrate and cofactor-binding domain mutants in a thermophilic alcohol dehydrogenase uncover an extended network of communication
PMID 25314615doi:10.1021/ja506667kOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (18 values) from this paper: thermophilic alcohol dehydrogenase (ht-ADH) from Bacillus stearothermophilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thermophilic alcohol dehydrogenase (ht-ADH)variant W49F/W167Y/V260A (W87in:V260A) | Bacillus stearothermophilus | benzyl alcohol | 1.4 | 8.2 | 0.17 calc. | 30 °C | Open record: thermophilic alcohol dehydrogenase (ht-ADH) variant W49F/W167Y/V260A (W87in:V260A), benzyl alcohol |
| thermophilic alcohol dehydrogenase (ht-ADH)variant W49F/W167Y/V260A (W87in:V260A) | Bacillus stearothermophilus | nicotinamide adenine dinucleotide | — | 8.3 | — | 30 °C | Open record: thermophilic alcohol dehydrogenase (ht-ADH) variant W49F/W167Y/V260A (W87in:V260A), nicotinamide adenine dinucleotide |
| thermophilic alcohol dehydrogenase (ht-ADH)variant W49F/W87F/V260A (W167in:V260A) | Bacillus stearothermophilus | benzyl alcohol | 1 | 12.9 | 0.078 calc. | 30 °C | Open record: thermophilic alcohol dehydrogenase (ht-ADH) variant W49F/W87F/V260A (W167in:V260A), benzyl alcohol |
| thermophilic alcohol dehydrogenase (ht-ADH)variant W49F/W87F/V260A (W167in:V260A) | Bacillus stearothermophilus | nicotinamide adenine dinucleotide | — | 10.2 | — | 30 °C | Open record: thermophilic alcohol dehydrogenase (ht-ADH) variant W49F/W87F/V260A (W167in:V260A), nicotinamide adenine dinucleotide |
| thermophilic alcohol dehydrogenase (ht-ADH)variant Y25A/W49F/W167Y (W87in:Y25A) | Bacillus stearothermophilus | benzyl alcohol | 2.5 | 0.8 | 3.1 calc. | 30 °C | Open record: thermophilic alcohol dehydrogenase (ht-ADH) variant Y25A/W49F/W167Y (W87in:Y25A), benzyl alcohol |
| thermophilic alcohol dehydrogenase (ht-ADH)variant Y25A/W49F/W167Y (W87in:Y25A) | Bacillus stearothermophilus | nicotinamide adenine dinucleotide | — | 0.5 | — | 30 °C | Open record: thermophilic alcohol dehydrogenase (ht-ADH) variant Y25A/W49F/W167Y (W87in:Y25A), nicotinamide adenine dinucleotide |
| thermophilic alcohol dehydrogenase (ht-ADH)variant Y25A/W49F/W167Y/V260A (W87in:Y25A:V260A) | Bacillus stearothermophilus | benzyl alcohol | 1.9 | 5.4 | 0.35 calc. | 30 °C | Open record: thermophilic alcohol dehydrogenase (ht-ADH) variant Y25A/W49F/W167Y/V260A (W87in:Y25A:V260A), benzyl alcohol |
| thermophilic alcohol dehydrogenase (ht-ADH)variant Y25A/W49F/W167Y/V260A (W87in:Y25A:V260A) | Bacillus stearothermophilus | nicotinamide adenine dinucleotide | — | 9.7 | — | 30 °C | Open record: thermophilic alcohol dehydrogenase (ht-ADH) variant Y25A/W49F/W167Y/V260A (W87in:Y25A:V260A), nicotinamide adenine dinucleotide |
| thermophilic alcohol dehydrogenase (ht-ADH)variant Y25A/W49F/W87F (W167in:Y25A) | Bacillus stearothermophilus | benzyl alcohol | 6.3 | 10.9 | 0.58 calc. | 30 °C | Open record: thermophilic alcohol dehydrogenase (ht-ADH) variant Y25A/W49F/W87F (W167in:Y25A), benzyl alcohol |
| thermophilic alcohol dehydrogenase (ht-ADH)variant Y25A/W49F/W87F (W167in:Y25A) | Bacillus stearothermophilus | nicotinamide adenine dinucleotide | — | 0.9 | — | 30 °C | Open record: thermophilic alcohol dehydrogenase (ht-ADH) variant Y25A/W49F/W87F (W167in:Y25A), nicotinamide adenine dinucleotide |
| thermophilic alcohol dehydrogenase (ht-ADH)variant Y25A/W49F/W87F/V260A (W167in:Y25A:V260A) | Bacillus stearothermophilus | benzyl alcohol | 2.5 | 10.1 | 0.25 calc. | 30 °C | Open record: thermophilic alcohol dehydrogenase (ht-ADH) variant Y25A/W49F/W87F/V260A (W167in:Y25A:V260A), benzyl alcohol |
| thermophilic alcohol dehydrogenase (ht-ADH)variant Y25A/W49F/W87F/V260A (W167in:Y25A:V260A) | Bacillus stearothermophilus | nicotinamide adenine dinucleotide | — | 14.8 | — | 30 °C | Open record: thermophilic alcohol dehydrogenase (ht-ADH) variant Y25A/W49F/W87F/V260A (W167in:Y25A:V260A), nicotinamide adenine dinucleotide |