Values CatLog read from this paper
Uncovering divergent evolution of α/β-hydrolases: a surprising residue substitution needed to convert Hevea brasiliensis hydroxynitrile lyase into an esterase
PMID 25346843doi:10.1039/c4sc01544dOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (57 values) from this paper: hydroxynitrile lyase (HbHNL), esterase (PFE), salicylic acid binding protein 2 (SABP2) from Hevea brasiliensis, Pseudomonas fluorescens, Nicotiana tabacum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| esterase (PFE) | Pseudomonas fluorescens | p-nitrophenyl acetate | 24.3 | 0.45 | 540 | pH 7.2, 25 °C | Open record: esterase (PFE), p-nitrophenyl acetate |
| hydroxynitrile lyase (HbHNL) | Hevea brasiliensis | acetone cyanohydrin | 65 | 0.4 | 162 | pH 5 | Open record: hydroxynitrile lyase (HbHNL), acetone cyanohydrin |
| hydroxynitrile lyase (HbHNL) | Hevea brasiliensis | mandelonitrile | 24 | 3.3 | 7.3 | pH 5, 25 °C | Open record: hydroxynitrile lyase (HbHNL), mandelonitrile |
| hydroxynitrile lyase (HbHNL) | Hevea brasiliensis | p-nitrophenyl acetate | 0.0018 | 1 | 0.0018 | pH 7.2, 25 °C | Open record: hydroxynitrile lyase (HbHNL), p-nitrophenyl acetate |
| hydroxynitrile lyase (HbHNL)variant E79H | Hevea brasiliensis | p-nitrophenyl acetate | 0.0013 | 0.19 | 0.0068 | pH 7.2, 25 °C | Open record: hydroxynitrile lyase (HbHNL) variant E79H, p-nitrophenyl acetate |
| hydroxynitrile lyase (HbHNL)variant E79H/K236M | Hevea brasiliensis | p-nitrophenyl acetate | 0.0053 | 0.21 | 0.0257 | pH 7.2, 25 °C | Open record: hydroxynitrile lyase (HbHNL) variant E79H/K236M, p-nitrophenyl acetate |
| hydroxynitrile lyase (HbHNL)variant K236G/E79H | Hevea brasiliensis | p-nitrophenyl acetate | 0.0062 | 0.16 | 0.0377 | pH 7.2, 25 °C | Open record: hydroxynitrile lyase (HbHNL) variant K236G/E79H, p-nitrophenyl acetate |
| hydroxynitrile lyase (HbHNL)variant K236M | Hevea brasiliensis | p-nitrophenyl acetate | 0.0123 | 0.58 | 0.0212 | pH 7.2, 25 °C | Open record: hydroxynitrile lyase (HbHNL) variant K236M, p-nitrophenyl acetate |
| hydroxynitrile lyase (HbHNL)variant T11G | Hevea brasiliensis | p-nitrophenyl acetate | 0.0015 | 0.2 | 0.0075 | pH 7.2, 25 °C | Open record: hydroxynitrile lyase (HbHNL) variant T11G, p-nitrophenyl acetate |
| hydroxynitrile lyase (HbHNL)variant T11G/E79H | Hevea brasiliensis | p-nitrophenyl acetate | 0.0097 | 0.14 | 0.0693 | pH 7.2, 25 °C | Open record: hydroxynitrile lyase (HbHNL) variant T11G/E79H, p-nitrophenyl acetate |
| hydroxynitrile lyase (HbHNL)variant T11G/K236G | Hevea brasiliensis | p-nitrophenyl acetate | 0.0022 | 0.15 | 0.0143 | pH 7.2, 25 °C | Open record: hydroxynitrile lyase (HbHNL) variant T11G/K236G, p-nitrophenyl acetate |
| hydroxynitrile lyase (HbHNL)variant T11G/K236G/E79H | Hevea brasiliensis | acetone cyanohydrin | 0.097 | 6.5 | 0.0148 | pH 5 | Open record: hydroxynitrile lyase (HbHNL) variant T11G/K236G/E79H, acetone cyanohydrin |
| hydroxynitrile lyase (HbHNL)variant T11G/K236G/E79H | Hevea brasiliensis | mandelonitrile | 0.135 | 3.2 | 0.042 | pH 5, 25 °C | Open record: hydroxynitrile lyase (HbHNL) variant T11G/K236G/E79H, mandelonitrile |
| hydroxynitrile lyase (HbHNL)variant T11G/K236G/E79H | Hevea brasiliensis | methyl benzoate | 0.0025 | — | 2.7 × 10⁻⁴ | pH 7.2 | Open record: hydroxynitrile lyase (HbHNL) variant T11G/K236G/E79H, methyl benzoate |
| hydroxynitrile lyase (HbHNL)variant T11G/K236G/E79H | Hevea brasiliensis | methyl salicylate | — | — | 1.22 × 10⁻⁴ | pH 7.2 | Open record: hydroxynitrile lyase (HbHNL) variant T11G/K236G/E79H, methyl salicylate |
| hydroxynitrile lyase (HbHNL)variant T11G/K236G/E79H | Hevea brasiliensis | p-nitrophenyl acetate | 0.027 | 0.16 | 0.168 | pH 7.2, 25 °C | Open record: hydroxynitrile lyase (HbHNL) variant T11G/K236G/E79H, p-nitrophenyl acetate |
| hydroxynitrile lyase (HbHNL)variant T11G/K236G/E79H | Hevea brasiliensis | phenyl acetate | 0.02 | 2.8 | 0.0068 | pH 7.2 | Open record: hydroxynitrile lyase (HbHNL) variant T11G/K236G/E79H, phenyl acetate |
| hydroxynitrile lyase (HbHNL)variant T11G/K236M | Hevea brasiliensis | p-nitrophenyl acetate | 0.01 | 0.55 | 0.018 | pH 7.2, 25 °C | Open record: hydroxynitrile lyase (HbHNL) variant T11G/K236M, p-nitrophenyl acetate |
| hydroxynitrile lyase (HbHNL)variant T11G/K236M/E79H | Hevea brasiliensis | p-nitrophenyl acetate | 0.0098 | 0.14 | 0.07 | pH 7.2, 25 °C | Open record: hydroxynitrile lyase (HbHNL) variant T11G/K236M/E79H, p-nitrophenyl acetate |
| salicylic acid binding protein 2 (SABP2) | Nicotiana tabacum | p-nitrophenyl acetate | 2 | 1.4 | 1.43 | pH 7.2, 25 °C | Open record: salicylic acid binding protein 2 (SABP2), p-nitrophenyl acetate |