Values CatLog read from this paper
Switching kinetic mechanism and putative proton donor by directed mutagenesis of glutathione reductase
PMID 2540822doi:10.1021/bi00429a047Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 15 records (23 values) from this paper: glutathione reductase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glutathione reductase | Escherichia coli | glutathione | 50 | 1.3 | 38 calc. | 30 °C | Open record: glutathione reductase, glutathione |
| glutathione reductase | Escherichia coli | glutathione disulfide | 60 | 0.097 | 620 calc. | 30 °C | Open record: glutathione reductase, glutathione disulfide |
| glutathione reductase | Escherichia coli | NADP+ | — | 0.12 | — | 30 °C | Open record: glutathione reductase, NADP+ |
| glutathione reductase | Escherichia coli | NADPH | — | 0.038 | — | 30 °C | Open record: glutathione reductase, NADPH |
| glutathione reductasevariant H439Q | Escherichia coli | glutathione disulfide | 2.3 | 0.31 | 7.5 calc. | 30 °C | Open record: glutathione reductase variant H439Q, glutathione disulfide |
| glutathione reductasevariant Y177F | Escherichia coli | glutathione | 23.3 | 1.2 | 19 calc. | 30 °C | Open record: glutathione reductase variant Y177F, glutathione |
| glutathione reductasevariant Y177F | Escherichia coli | glutathione disulfide | 517 | 0.053 | 9.7 × 10³ calc. | 30 °C | Open record: glutathione reductase variant Y177F, glutathione disulfide |
| glutathione reductasevariant Y177F | Escherichia coli | NADP+ | — | 0.07 | — | 30 °C | Open record: glutathione reductase variant Y177F, NADP+ |
| glutathione reductasevariant Y177F | Escherichia coli | NADPH | — | 0.024 | — | 30 °C | Open record: glutathione reductase variant Y177F, NADPH |
| glutathione reductasevariant Y177G | Escherichia coli | glutathione disulfide | 4.7 | 0.005 | 930 calc. | 30 °C | Open record: glutathione reductase variant Y177G, glutathione disulfide |
| glutathione reductasevariant Y177G | Escherichia coli | NADPH | — | 0.018 | — | 30 °C | Open record: glutathione reductase variant Y177G, NADPH |
| glutathione reductasevariant Y177S | Escherichia coli | glutathione | 1.67 | 0.7 | 2.4 calc. | 30 °C | Open record: glutathione reductase variant Y177S, glutathione |
| glutathione reductasevariant Y177S | Escherichia coli | glutathione disulfide | 137 | 0.002 | 6.8 × 10⁴ calc. | 30 °C | Open record: glutathione reductase variant Y177S, glutathione disulfide |
| glutathione reductasevariant Y177S | Escherichia coli | NADP+ | — | 0.08 | — | 30 °C | Open record: glutathione reductase variant Y177S, NADP+ |
| glutathione reductasevariant Y177S | Escherichia coli | NADPH | — | 0.03 | — | 30 °C | Open record: glutathione reductase variant Y177S, NADPH |