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Catalytic surface radical in dye-decolorizing peroxidase: a computational, spectroscopic and site-directed mutagenesis study

PMID 25495127doi:10.1042/bj20141211Open in Browse

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CatLog read 88 records (205 values) from this paper: dye-decolorizing peroxidase DyP-I from Auricularia auricula-judae.

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EC 1.11.1.19

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)high-turnover site constants (Table 1 "high turnover" row)	224	0.121	1.85 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)low-turnover site constants (Table 1 "low turnover" row)	7.4	0.0031	2.37 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)mature DyP-I coding sequence	224	—	1.85 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)mature DyP-I coding sequence	7.4	—	2.37 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	2,6-dimethoxyphenolhigh-turnover site constants (Table 1 "high turnover" row)	120	0.703	200	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	2,6-dimethoxyphenollow-turnover site constants (Table 1 "low turnover" row)	8	0.006	1.35 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	2,6-dimethoxyphenolmature DyP-I coding sequence	120	—	200	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	2,6-dimethoxyphenolmature DyP-I coding sequence	8	—	1.35 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	Reactive Black 5	4.8	0.0156	310	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, Reactive Black 5
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	Reactive Black 5 (4-amino-5-hydroxy-3,6-bis((E)-(4-((2-(sulfooxy)ethyl)sulfonyl)phenyl)diazenyl)naphthalene-2,7-disulfonic acid)	4.8	—	310	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, Reactive Black 5 (4-amino-5-hydroxy-3,6-bis((E)-(4-((2-(sulfooxy)ethyl)sulfonyl)phenyl)diazenyl)naphthalene-2,7-disulfonic acid)
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	Reactive Blue 19high-turnover site constants (Table 1 "high turnover" row)	224	0.09	2.46 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, Reactive Blue 19
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	Reactive Blue 19low-turnover site constants (Table 1 "low turnover" row)	32	0.014	2.23 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, Reactive Blue 19
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)	224	—	2.46 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)	32	—	2.23 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	veratryl alcohol	—	—	0.096	pH 2.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, veratryl alcohol
dye-decolorizing peroxidase DyP-I	Auricularia auricula-judae	veratryl alcohol (3,4-dimethoxybenzyl alcohol)	—	—	0.096	pH 2.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I, veratryl alcohol (3,4-dimethoxybenzyl alcohol)
dye-decolorizing peroxidase DyP-Ivariant G169L	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)high-turnover site constants (Table 1 "high turnover" row)	96	0.025	3.77 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant G169L, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant G169L	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)mature DyP-I coding sequence	96	—	3.77 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant G169L, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant G169L	Auricularia auricula-judae	2,6-dimethoxyphenolhigh-turnover site constants (Table 1 "high turnover" row)	88	0.353	200	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant G169L, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant G169L	Auricularia auricula-judae	2,6-dimethoxyphenollow-turnover site constants (Table 1 "low turnover" row)	9.9	0.006	1.6 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant G169L, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant G169L	Auricularia auricula-judae	2,6-dimethoxyphenolmature DyP-I coding sequence	88	—	200	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant G169L, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant G169L	Auricularia auricula-judae	2,6-dimethoxyphenolmature DyP-I coding sequence	9.9	—	1.6 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant G169L, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant G169L	Auricularia auricula-judae	Reactive Black 5	1.2	0.0058	217	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant G169L, Reactive Black 5
dye-decolorizing peroxidase DyP-Ivariant G169L	Auricularia auricula-judae	Reactive Black 5 (4-amino-5-hydroxy-3,6-bis((E)-(4-((2-(sulfooxy)ethyl)sulfonyl)phenyl)diazenyl)naphthalene-2,7-disulfonic acid)	1.2	—	217	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant G169L, Reactive Black 5 (4-amino-5-hydroxy-3,6-bis((E)-(4-((2-(sulfooxy)ethyl)sulfonyl)phenyl)diazenyl)naphthalene-2,7-disulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant G169L	Auricularia auricula-judae	Reactive Blue 19	106	0.01	1.24 × 10⁴	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant G169L, Reactive Blue 19
dye-decolorizing peroxidase DyP-Ivariant G169L	Auricularia auricula-judae	Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)	106	—	1.24 × 10⁴	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant G169L, Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant G169L	Auricularia auricula-judae	veratryl alcohol	—	—	0.13	pH 2.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant G169L, veratryl alcohol
dye-decolorizing peroxidase DyP-Ivariant G169L	Auricularia auricula-judae	veratryl alcohol (3,4-dimethoxybenzyl alcohol)	—	—	0.13	pH 2.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant G169L, veratryl alcohol (3,4-dimethoxybenzyl alcohol)
dye-decolorizing peroxidase DyP-Ivariant W377S	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)high-turnover site constants (Table 1 "high turnover" row)	171	2.75	62	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant W377S, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant W377S	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)low-turnover site constants (Table 1 "low turnover" row)	14	0.03	472	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant W377S, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant W377S	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)mature DyP-I coding sequence	171	—	62	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant W377S, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant W377S	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)mature DyP-I coding sequence	14	—	472	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant W377S, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant W377S	Auricularia auricula-judae	2,6-dimethoxyphenollow-turnover site constants (Table 1 "low turnover" row)	6.4	3.56	1.8	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant W377S, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant W377S	Auricularia auricula-judae	2,6-dimethoxyphenolmature DyP-I coding sequence	6.4	—	1.8	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant W377S, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant W377S	Auricularia auricula-judae	Reactive Black 5	0.7	0.0106	68	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant W377S, Reactive Black 5
dye-decolorizing peroxidase DyP-Ivariant W377S	Auricularia auricula-judae	Reactive Black 5 (4-amino-5-hydroxy-3,6-bis((E)-(4-((2-(sulfooxy)ethyl)sulfonyl)phenyl)diazenyl)naphthalene-2,7-disulfonic acid)	0.7	—	68	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant W377S, Reactive Black 5 (4-amino-5-hydroxy-3,6-bis((E)-(4-((2-(sulfooxy)ethyl)sulfonyl)phenyl)diazenyl)naphthalene-2,7-disulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant W377S	Auricularia auricula-judae	Reactive Blue 19	8.9	0.0039	2.24 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant W377S, Reactive Blue 19
dye-decolorizing peroxidase DyP-Ivariant W377S	Auricularia auricula-judae	Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)	8.9	—	2.24 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant W377S, Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant W377S	Auricularia auricula-judae	veratryl alcohol	0.23	7.36	0.032	pH 2.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant W377S, veratryl alcohol
dye-decolorizing peroxidase DyP-Ivariant W377S	Auricularia auricula-judae	veratryl alcohol (3,4-dimethoxybenzyl alcohol)	0.23	—	0.032	pH 2.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant W377S, veratryl alcohol (3,4-dimethoxybenzyl alcohol)
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)high-turnover site constants (Table 1 "high turnover" row)	286	0.173	1.65 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)low-turnover site constants (Table 1 "low turnover" row)	21	0.017	1.26 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)mature DyP-I coding sequence	286	—	1.65 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)mature DyP-I coding sequence	21	—	1.26 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	2,6-dimethoxyphenolhigh-turnover site constants (Table 1 "high turnover" row)	216	4.72	46	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	2,6-dimethoxyphenollow-turnover site constants (Table 1 "low turnover" row)	3.9	7 × 10⁻⁴	5.9 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	2,6-dimethoxyphenolmature DyP-I coding sequence	216	—	46	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	2,6-dimethoxyphenolmature DyP-I coding sequence	3.9	—	5.9 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	Reactive Black 5	9.1	0.039	233	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, Reactive Black 5
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	Reactive Black 5 (4-amino-5-hydroxy-3,6-bis((E)-(4-((2-(sulfooxy)ethyl)sulfonyl)phenyl)diazenyl)naphthalene-2,7-disulfonic acid)	9.1	—	233	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, Reactive Black 5 (4-amino-5-hydroxy-3,6-bis((E)-(4-((2-(sulfooxy)ethyl)sulfonyl)phenyl)diazenyl)naphthalene-2,7-disulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	Reactive Blue 19high-turnover site constants (Table 1 "high turnover" row)	220	0.13	1.68 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, Reactive Blue 19
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	Reactive Blue 19low-turnover site constants (Table 1 "low turnover" row)	18	0.0058	3.2 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, Reactive Blue 19
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)	220	—	1.68 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)	18	—	3.2 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	veratryl alcohol	—	—	0.095	pH 2.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, veratryl alcohol
dye-decolorizing peroxidase DyP-Ivariant Y147F/Y337F	Auricularia auricula-judae	veratryl alcohol (3,4-dimethoxybenzyl alcohol)	—	—	0.095	pH 2.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147F/Y337F, veratryl alcohol (3,4-dimethoxybenzyl alcohol)
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)high-turnover site constants (Table 1 "high turnover" row)	311	0.366	850	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)low-turnover site constants (Table 1 "low turnover" row)	21	0.02	1.01 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)mature DyP-I coding sequence	311	—	850	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)mature DyP-I coding sequence	21	—	1.01 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	2,6-dimethoxyphenolhigh-turnover site constants (Table 1 "high turnover" row)	71	0.763	93	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	2,6-dimethoxyphenollow-turnover site constants (Table 1 "low turnover" row)	15	0.038	397	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	2,6-dimethoxyphenolmature DyP-I coding sequence	71	—	93	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	2,6-dimethoxyphenolmature DyP-I coding sequence	15	—	397	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	Reactive Black 5	8.3	0.0159	525	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, Reactive Black 5
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	Reactive Black 5 (4-amino-5-hydroxy-3,6-bis((E)-(4-((2-(sulfooxy)ethyl)sulfonyl)phenyl)diazenyl)naphthalene-2,7-disulfonic acid)	8.3	—	525	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, Reactive Black 5 (4-amino-5-hydroxy-3,6-bis((E)-(4-((2-(sulfooxy)ethyl)sulfonyl)phenyl)diazenyl)naphthalene-2,7-disulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	Reactive Blue 19high-turnover site constants (Table 1 "high turnover" row)	175	0.095	1.86 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, Reactive Blue 19
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	Reactive Blue 19low-turnover site constants (Table 1 "low turnover" row)	26	0.0064	4.07 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, Reactive Blue 19
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)	175	—	1.86 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)	26	—	4.07 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	veratryl alcohol	—	—	0.101	pH 2.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, veratryl alcohol
dye-decolorizing peroxidase DyP-Ivariant Y147S	Auricularia auricula-judae	veratryl alcohol (3,4-dimethoxybenzyl alcohol)	—	—	0.101	pH 2.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y147S, veratryl alcohol (3,4-dimethoxybenzyl alcohol)
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)high-turnover site constants (Table 1 "high turnover" row)	288	0.239	1.2 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)low-turnover site constants (Table 1 "low turnover" row)	2.3	0.002	1.1 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)mature DyP-I coding sequence	288	—	1.2 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)mature DyP-I coding sequence	2.3	—	1.1 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	2,6-dimethoxyphenolhigh-turnover site constants (Table 1 "high turnover" row)	228	2.84	80	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	2,6-dimethoxyphenollow-turnover site constants (Table 1 "low turnover" row)	8.4	0.0031	2.73 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	2,6-dimethoxyphenolmature DyP-I coding sequence	228	—	80	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	2,6-dimethoxyphenolmature DyP-I coding sequence	8.4	—	2.73 × 10³	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, 2,6-dimethoxyphenol
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	Reactive Black 5	5.2	0.0134	400	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, Reactive Black 5
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	Reactive Black 5 (4-amino-5-hydroxy-3,6-bis((E)-(4-((2-(sulfooxy)ethyl)sulfonyl)phenyl)diazenyl)naphthalene-2,7-disulfonic acid)	5.2	—	400	pH 3, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, Reactive Black 5 (4-amino-5-hydroxy-3,6-bis((E)-(4-((2-(sulfooxy)ethyl)sulfonyl)phenyl)diazenyl)naphthalene-2,7-disulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	Reactive Blue 19high-turnover site constants (Table 1 "high turnover" row)	240	0.083	2.7 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, Reactive Blue 19
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	Reactive Blue 19low-turnover site constants (Table 1 "low turnover" row)	26	0.0074	3.37 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, Reactive Blue 19
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)	240	—	2.7 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)	26	—	3.37 × 10³	pH 3.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, Reactive Blue 19 (1-amino-9,10-dioxo-4-((3-((2-(sulfooxy)ethyl)sulfonyl)phenyl)amino)-9,10-dihydroanthracene-2-sulfonic acid)
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	veratryl alcohol	—	—	0.079	pH 2.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, veratryl alcohol
dye-decolorizing peroxidase DyP-Ivariant Y337S	Auricularia auricula-judae	veratryl alcohol (3,4-dimethoxybenzyl alcohol)	—	—	0.079	pH 2.5, 25 °C	Open record: dye-decolorizing peroxidase DyP-I variant Y337S, veratryl alcohol (3,4-dimethoxybenzyl alcohol)