CatLogenzyme kinetics catalog

Values CatLog read from this paper

Enhancing the heat stability and kinetic parameters of the maize endosperm ADP-glucose pyrophosphorylase using iterative saturation mutagenesis

PMID 25600571doi:10.1016/j.abb.2015.01.008Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 30 records (32 values) from this paper: ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer) from Zea mays.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.7.7.27

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)Zea maysadenosine 5'-triphosphate—0.069—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)Zea maysalpha-D-glucose 1-phosphate—0.039—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant D161GZea maysadenosine 5'-triphosphatewith 5 mM 3-PGA (activator)—0.044—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant D161GZea maysadenosine 5'-triphosphateno 3-PGA; ATP varied 0.25-7.5 mM at fixed G-1-P 5.0-30 mM48.80.0431.1 × 10³ calc.pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant D161GZea maysalpha-D-glucose 1-phosphatewith 5 mM 3-PGA (activator)—0.06—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant D161GZea maysalpha-D-glucose 1-phosphateno 3-PGA; ATP varied 0.25-7.5 mM at fixed G-1-P 5.0-30 mM—6.8—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96GZea maysadenosine 5'-triphosphate—0.055—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96GZea maysalpha-D-glucose 1-phosphate—0.064—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96G/D161GZea maysadenosine 5'-triphosphate—0.031—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96G/D161GZea maysalpha-D-glucose 1-phosphate—0.021—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96G/D161G/A443RZea maysadenosine 5'-triphosphatewith 5 mM 3-PGA (activator)—0.057—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96G/D161G/A443RZea maysadenosine 5'-triphosphateno 3-PGA; ATP varied 0.25-7.5 mM at fixed G-1-P 5.0-30 mM72.40.1720 calc.pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96G/D161G/A443RZea maysadenosine 5'-triphosphateno 3-PGA; Pi inhibition experiment (Pi varied)—0.54—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96G/D161G/A443RZea maysadenosine 5'-triphosphatewith 5 mM 3-PGA; Pi inhibition experiment (Pi varied)—0.11—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96G/D161G/A443RZea maysalpha-D-glucose 1-phosphatewith 5 mM 3-PGA (activator)—0.038—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96G/D161G/A443RZea maysalpha-D-glucose 1-phosphateno 3-PGA; ATP varied 0.25-7.5 mM at fixed G-1-P 5.0-30 mM—8.74—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96G/D161G/A443RZea maysalpha-D-glucose 1-phosphateno 3-PGA; Pi inhibition experiment (Pi varied)—13.1—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96G/D161G/A443RZea maysalpha-D-glucose 1-phosphatewith 5 mM 3-PGA; Pi inhibition experiment (Pi varied)—0.085—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96G/D161G/A443R/I285VZea maysadenosine 5'-triphosphate—0.046—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96G/D161G/A443R/I285VZea maysalpha-D-glucose 1-phosphate—0.024—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96G/D161G/A443R/Q289AZea maysadenosine 5'-triphosphate—0.057—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96G/D161G/A443R/Q289AZea maysalpha-D-glucose 1-phosphate—0.037—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96RZea maysadenosine 5'-triphosphate—0.072—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96RZea maysalpha-D-glucose 1-phosphate—0.032—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96R/D161GZea maysadenosine 5'-triphosphate—0.063—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96R/D161GZea maysalpha-D-glucose 1-phosphate—0.038—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96R/D161G/A443RZea maysadenosine 5'-triphosphate—0.062—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96R/D161G/A443RZea maysalpha-D-glucose 1-phosphate—0.028—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96R/D161G/A443R/S444RZea maysadenosine 5'-triphosphate—0.038—pH 7.4, 37 °COpen
ADP-glucose pyrophosphorylase (maize endosperm, Sh2/Bt2 heterotetramer)variant Q96R/D161G/A443R/S444RZea maysalpha-D-glucose 1-phosphate—0.025—pH 7.4, 37 °COpen