CatLogenzyme kinetics catalog

Values CatLog read from this paper

Open-close structural change upon ligand binding and two magnesium ions required for the catalysis of N-acetylhexosamine 1-kinase

PMID 25644306doi:10.1016/j.bbapap.2015.01.011Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 8 records (16 values) from this paper: N-acetylhexosamine 1-kinase (NahK) from Bifidobacterium longum.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.7.1.162

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
N-acetylhexosamine 1-kinase (NahK)Bifidobacterium longumadenosine 5'-triphosphate16.30.1160 calc.pH 8, 37 °COpen
N-acetylhexosamine 1-kinase (NahK)Bifidobacterium longumN-acetyl-D-glucosamine9.91.85.5 calc.pH 8, 37 °COpen
N-acetylhexosamine 1-kinase (NahK)variant K210ABifidobacterium longumadenosine 5'-triphosphate0.120.00524 calc.pH 8, 37 °COpen
N-acetylhexosamine 1-kinase (NahK)variant K210ABifidobacterium longumN-acetyl-D-glucosamine0.110.350.31 calc.pH 8, 37 °COpen
N-acetylhexosamine 1-kinase (NahK)variant N213ABifidobacterium longumadenosine 5'-triphosphate5.0 mM MgCl2; GlcNAc fixed at 20 mM6.40.97.1 calc.pH 8, 37 °COpen
N-acetylhexosamine 1-kinase (NahK)variant N213ABifidobacterium longumadenosine 5'-triphosphate50 mM MgCl2 (high Mg2+); GlcNAc fixed at 40 mM8.80.038230 calc.pH 8, 37 °COpen
N-acetylhexosamine 1-kinase (NahK)variant N213ABifidobacterium longumN-acetyl-D-glucosamine5.0 mM MgCl25.92.52.4 calc.pH 8, 37 °COpen
N-acetylhexosamine 1-kinase (NahK)variant N213ABifidobacterium longumN-acetyl-D-glucosamine50 mM MgCl2 (high Mg2+)13.63.144.33 calc.pH 8, 37 °COpen