CatLogenzyme kinetics catalog

Values CatLog read from this paper

Identification of a determinant for strict NADP(H)-specificity and high sensitivity to mixed-type steroid inhibitor of rabbit aldo-keto reductase 1C33 by site-directed mutagenesis

PMID 25660042doi:10.1016/j.abb.2015.01.026Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 48 records (96 values) from this paper: aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase), aldo-keto reductase 1C32 (rabbit 3alpha/20alpha-hydroxysteroid dehydrogenase) from Oryctolagus cuniculus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 14 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.357

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
aldo-keto reductase 1C32 (rabbit 3alpha/20alpha-hydroxysteroid dehydrogenase)Oryctolagus cuniculusNAD+0.250.0992.5pH 7.4, 25 °COpen
aldo-keto reductase 1C32 (rabbit 3alpha/20alpha-hydroxysteroid dehydrogenase)Oryctolagus cuniculusNADH0.0730.032.5pH 7.4, 25 °COpen
aldo-keto reductase 1C32 (rabbit 3alpha/20alpha-hydroxysteroid dehydrogenase)Oryctolagus cuniculusNADP+0.1350.001135pH 7.4, 25 °COpen
aldo-keto reductase 1C32 (rabbit 3alpha/20alpha-hydroxysteroid dehydrogenase)Oryctolagus cuniculusNADPH0.0227 × 10⁻⁴32pH 7.4, 25 °COpen
aldo-keto reductase 1C32 (rabbit 3alpha/20alpha-hydroxysteroid dehydrogenase)Oryctolagus cuniculuspyridine-3-aldehydeNADPH-linked reduction; substrate varied with 0.1 mM NADPH—0.017—pH 7.4, 25 °COpen
aldo-keto reductase 1C32 (rabbit 3alpha/20alpha-hydroxysteroid dehydrogenase)Oryctolagus cuniculuspyridine-3-aldehydeNADH-linked reduction; substrate varied with 0.1 mM NADH—0.353—pH 7.4, 25 °COpen
aldo-keto reductase 1C32 (rabbit 3alpha/20alpha-hydroxysteroid dehydrogenase)Oryctolagus cuniculusβ-ionolNADP+-linked oxidation; substrate varied with 0.25 mM NADP+—0.037—pH 7.4, 25 °COpen
aldo-keto reductase 1C32 (rabbit 3alpha/20alpha-hydroxysteroid dehydrogenase)Oryctolagus cuniculusβ-ionolNAD+-linked oxidation; substrate varied with 1 mM NAD+—0.093—pH 7.4, 25 °COpen
aldo-keto reductase 1C32 (rabbit 3alpha/20alpha-hydroxysteroid dehydrogenase)variant S217FOryctolagus cuniculusNAD+0.01171.760.0067pH 7.4, 25 °COpen
aldo-keto reductase 1C32 (rabbit 3alpha/20alpha-hydroxysteroid dehydrogenase)variant S217FOryctolagus cuniculusNADP+0.270.01418pH 7.4, 25 °COpen
aldo-keto reductase 1C32 (rabbit 3alpha/20alpha-hydroxysteroid dehydrogenase)variant S217FOryctolagus cuniculusNADPH0.1070.00427pH 7.4, 25 °COpen
aldo-keto reductase 1C32 (rabbit 3alpha/20alpha-hydroxysteroid dehydrogenase)variant S217FOryctolagus cuniculuspyridine-3-aldehyde—0.269—pH 7.4, 25 °COpen
aldo-keto reductase 1C32 (rabbit 3alpha/20alpha-hydroxysteroid dehydrogenase)variant S217FOryctolagus cuniculusβ-ionolNADP+-linked oxidation; substrate varied with 0.25 mM NADP+—0.161—pH 7.4, 25 °COpen
aldo-keto reductase 1C32 (rabbit 3alpha/20alpha-hydroxysteroid dehydrogenase)variant S217FOryctolagus cuniculusβ-ionolNAD+-linked oxidation; substrate varied with 2 mM NAD+—0.048—pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)Oryctolagus cuniculus5α-androstane-3α,17β-diolNADP+-linked oxidation; substrate varied with 0.25 mM NADP+—0.0015—pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)Oryctolagus cuniculus5α-androstane-3α,17β-diolNAD+-linked oxidation; substrate varied with 2 mM NAD+—0.018—pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)Oryctolagus cuniculusNAD+0.0470.970.048pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)Oryctolagus cuniculusNADP+0.850.01750pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)Oryctolagus cuniculusNADPH0.0770.00849pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)Oryctolagus cuniculuspyridine-3-aldehyde—0.32—pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculus5α-Androstan-17β-ol-3-one0.00381 × 10⁻⁴38 calc.pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculus5α-androstane-3α,17β-diolNADP+-linked oxidation; substrate varied with 0.25 mM NADP+—3.5 × 10⁻⁴—pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculus5α-androstane-3α,17β-diolNAD+-linked oxidation; substrate varied with 1 mM NAD+—0.0089—pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculus5α-Pregnan-20α-ol-3-one0.00251.6 × 10⁻⁴16 calc.pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculus5β-Androstan-3α-ol-17-one0.00385.8 × 10⁻⁴6.6 calc.pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculus5β-Androstane-3,17-dione0.00935.7 × 10⁻⁴16 calc.pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculus5β-Cholanic acid-3,7-dione0.020.00277.4 calc.pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculus5β-Pregnane-3,20-dione0.0075.2 × 10⁻⁴13 calc.pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculusChenodeoxycholic acid0.00450.00251.8 calc.pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculusCholic acid0.00180.0210.087 calc.pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculusDehydrolithocholic acid0.00629.2 × 10⁻⁴6.7 calc.pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculusGlycochenodeoxycholic acid0.00430.00212.1 calc.pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculusLithocholic acid0.0050.00281.8 calc.pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculusMethylglyoxal0.01530.0580.26 calc.pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculusNAD+0.0850.0811.05pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculusNADH0.1070.0333.2pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculusNADP+0.00572 × 10⁻⁴28pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculusNADPH0.01034 × 10⁻⁴27pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculuspyridine-3-aldehydeNADPH-linked reduction; substrate varied with 0.1 mM NADPH—0.071—pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculuspyridine-3-aldehydeNADH-linked reduction; substrate varied with 0.1 mM NADH—0.875—pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculusTaurodehydrocholic acid0.0060.00660.91 calc.pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant F217SOryctolagus cuniculusTaurolithocholic acid0.00539.2 × 10⁻⁴5.8 calc.pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant N272TOryctolagus cuniculus5α-androstane-3α,17β-diolNADP+-linked oxidation; substrate varied with 0.25 mM NADP+—0.0018—pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant N272TOryctolagus cuniculus5α-androstane-3α,17β-diolNAD+-linked oxidation; substrate varied with 2 mM NAD+—0.017—pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant N272TOryctolagus cuniculusNAD+0.04510.045pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant N272TOryctolagus cuniculusNADP+0.720.01742pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant N272TOryctolagus cuniculusNADPH0.10.00911pH 7.4, 25 °COpen
aldo-keto reductase 1C33 (rabbit 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase)variant N272TOryctolagus cuniculuspyridine-3-aldehyde—0.334—pH 7.4, 25 °COpen