Values CatLog read from this paper
A role for catalase-peroxidase large loop 2 revealed by deletion mutagenesis: control of active site water and ferric enzyme reactivity
PMID 25674665doi:10.1021/bi501221aOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 53 records (157 values) from this paper: catalase-peroxidase KatG from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| catalase-peroxidase KatG | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | — | — | 630 | pH 5, 23 °C | Open record: catalase-peroxidase KatG, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatG | Escherichia coli | hydrogen peroxide | 1.2 × 10⁴ | 3.7 | 3.3 × 10³ | pH 7, 23 °C | Open record: catalase-peroxidase KatG, hydrogen peroxide |
| catalase-peroxidase KatGvariant E291A | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 39.7 | 0.071 | 560 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant E291A, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant E291A | Escherichia coli | hydrogen peroxide | 1.67 × 10⁴ | 5.9 | 2.8 × 10³ | pH 7, 23 °C | Open record: catalase-peroxidase KatG variant E291A, hydrogen peroxide |
| catalase-peroxidase KatGvariant E291A | Escherichia coli | hydrogen peroxide | 40 | 0.1 | 390 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant E291A, hydrogen peroxide |
| catalase-peroxidase KatGvariant E291K | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 29.6 | 0.023 | 1.26 × 10³ | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant E291K, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant E291K | Escherichia coli | hydrogen peroxide | 9.5 × 10³ | 2.1 | 4.5 × 10³ | pH 7, 23 °C | Open record: catalase-peroxidase KatG variant E291K, hydrogen peroxide |
| catalase-peroxidase KatGvariant E291K | Escherichia coli | hydrogen peroxide | 34 | 0.06 | 600 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant E291K, hydrogen peroxide |
| catalase-peroxidase KatGvariant E291K/E292A | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 38 | 0.028 | 1.4 × 10³ | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant E291K/E292A, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant E291K/E292A | Escherichia coli | hydrogen peroxide | 1.22 × 10⁴ | 2.5 | 4.8 × 10³ | pH 7, 23 °C | Open record: catalase-peroxidase KatG variant E291K/E292A, hydrogen peroxide |
| catalase-peroxidase KatGvariant E291K/E292A | Escherichia coli | hydrogen peroxide | 35.3 | 0.06 | 620 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant E291K/E292A, hydrogen peroxide |
| catalase-peroxidase KatGvariant I290A | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 32.5 | 0.16 | 200 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant I290A, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant I290A | Escherichia coli | hydrogen peroxide | 1.03 × 10⁴ | 4 | 2.6 × 10³ | pH 7, 23 °C | Open record: catalase-peroxidase KatG variant I290A, hydrogen peroxide |
| catalase-peroxidase KatGvariant I290A | Escherichia coli | hydrogen peroxide | 26 | 0.09 | 310 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant I290A, hydrogen peroxide |
| catalase-peroxidase KatGvariant I290A/E291A/E292A/Q293A | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 70 | 0.029 | 2.4 × 10³ | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant I290A/E291A/E292A/Q293A, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant I290A/E291A/E292A/Q293A | Escherichia coli | hydrogen peroxide | 630 | 3.7 | 190 | pH 7, 23 °C | Open record: catalase-peroxidase KatG variant I290A/E291A/E292A/Q293A, hydrogen peroxide |
| catalase-peroxidase KatGvariant I290A/E291A/E292A/Q293A | Escherichia coli | hydrogen peroxide | 99 | 75 | 1.3 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant I290A/E291A/E292A/Q293A, hydrogen peroxide |
| catalase-peroxidase KatGvariant I290A/Q293A | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 13.3 | 0.037 | 350 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant I290A/Q293A, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant I290A/Q293A | Escherichia coli | hydrogen peroxide | 7.7 × 10³ | 4.2 | 1.8 × 10³ | pH 7, 23 °C | Open record: catalase-peroxidase KatG variant I290A/Q293A, hydrogen peroxide |
| catalase-peroxidase KatGvariant I290A/Q293A | Escherichia coli | hydrogen peroxide | 13.6 | 0.07 | 210 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant I290A/Q293A, hydrogen peroxide |
| catalase-peroxidase KatGvariant I290V | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 28.5 | 0.13 | 220 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant I290V, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant I290V | Escherichia coli | hydrogen peroxide | 8 × 10³ | 3.9 | 3.7 × 10³ | pH 7, 23 °C | Open record: catalase-peroxidase KatG variant I290V, hydrogen peroxide |
| catalase-peroxidase KatGvariant I290V | Escherichia coli | hydrogen peroxide | 26 | 0.08 | 350 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant I290V, hydrogen peroxide |
| catalase-peroxidase KatGvariant I290V/Q293V | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 22.5 | 0.081 | 280 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant I290V/Q293V, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant I290V/Q293V | Escherichia coli | hydrogen peroxide | 5.6 × 10³ | 3.2 | 1.8 × 10³ | pH 7, 23 °C | Open record: catalase-peroxidase KatG variant I290V/Q293V, hydrogen peroxide |
| catalase-peroxidase KatGvariant I290V/Q293V | Escherichia coli | hydrogen peroxide | 24 | 0.21 | 110 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant I290V/Q293V, hydrogen peroxide |
| catalase-peroxidase KatGvariant Q293A | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 29.3 | 0.08 | 370 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant Q293A, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant Q293A | Escherichia coli | hydrogen peroxide | 1.22 × 10⁴ | 4.6 | 2.7 × 10³ | pH 7, 23 °C | Open record: catalase-peroxidase KatG variant Q293A, hydrogen peroxide |
| catalase-peroxidase KatGvariant Q293A | Escherichia coli | hydrogen peroxide | 27.8 | 0.078 | 380 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant Q293A, hydrogen peroxide |
| catalase-peroxidase KatGvariant Q293E | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 20 | 0.05 | 390 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant Q293E, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant Q293E | Escherichia coli | hydrogen peroxide | 9.6 × 10³ | 8 | 1.3 × 10³ | pH 7, 23 °C | Open record: catalase-peroxidase KatG variant Q293E, hydrogen peroxide |
| catalase-peroxidase KatGvariant Q293E | Escherichia coli | hydrogen peroxide | 23.8 | 0.089 | 268 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant Q293E, hydrogen peroxide |
| catalase-peroxidase KatGvariant Q293V | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 15.2 | 0.09 | 160 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant Q293V, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant Q293V | Escherichia coli | hydrogen peroxide | 5.3 × 10³ | 3.1 | 1.7 × 10³ | pH 7, 23 °C | Open record: catalase-peroxidase KatG variant Q293V, hydrogen peroxide |
| catalase-peroxidase KatGvariant Q293V | Escherichia coli | hydrogen peroxide | 26 | 0.5 | 49 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant Q293V, hydrogen peroxide |
| catalase-peroxidase KatGvariant Δ288-293 | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 65.2 | 0.041 | 1.6 × 10³ | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant Δ288-293, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant Δ288-293 | Escherichia coli | hydrogen peroxide | 85 | 13 | 6.5 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant Δ288-293, hydrogen peroxide |
| catalase-peroxidase KatGvariant Δ290-293 | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 37.7 | 0.022 | 1.68 × 10³ | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant Δ290-293, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant Δ290-293 | Escherichia coli | hydrogen peroxide | 37 | 5 | 7.4 | pH 7, 23 °C | Open record: catalase-peroxidase KatG variant Δ290-293, hydrogen peroxide |
| catalase-peroxidase KatGvariant Δ290-293 | Escherichia coli | hydrogen peroxide | 684 | 366 | 2 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant Δ290-293, hydrogen peroxide |
| catalase-peroxidase KatGvariant Δ291 | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 68.5 | 0.062 | 1.1 × 10³ | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant Δ291, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant Δ291 | Escherichia coli | hydrogen peroxide | 2 × 10³ | 3.1 | 650 | pH 7, 23 °C | Open record: catalase-peroxidase KatG variant Δ291, hydrogen peroxide |
| catalase-peroxidase KatGvariant Δ291 | Escherichia coli | hydrogen peroxide | 184 | 41 | 4.6 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant Δ291, hydrogen peroxide |
| catalase-peroxidase KatGvariant Δ291-292 | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 54.5 | 0.02 | 2.8 × 10³ | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant Δ291-292, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant Δ291-292 | Escherichia coli | hydrogen peroxide | 273 | 107 | 2.7 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant Δ291-292, hydrogen peroxide |
| catalase-peroxidase KatGvariant ΔLL2 | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 27 | 0.013 | 2.1 × 10³ | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant ΔLL2, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant ΔLL2 | Escherichia coli | hydrogen peroxide | 27.9 | 2.2 | 12 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant ΔLL2, hydrogen peroxide |
| catalase-peroxidase KatGvariant ΔN280/ΔS299 | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 54 | 0.075 | 730 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant ΔN280/ΔS299, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant ΔN280/ΔS299 | Escherichia coli | hydrogen peroxide | 2.5 × 10³ | 3.3 | 790 | pH 7, 23 °C | Open record: catalase-peroxidase KatG variant ΔN280/ΔS299, hydrogen peroxide |
| catalase-peroxidase KatGvariant ΔN280/ΔS299 | Escherichia coli | hydrogen peroxide | 224 | 58 | 3.9 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant ΔN280/ΔS299, hydrogen peroxide |
| catalase-peroxidase KatGvariant ΔP285/ΔG294 | Escherichia coli | 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 29 | 0.069 | 420 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant ΔP285/ΔG294, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) |
| catalase-peroxidase KatGvariant ΔP285/ΔG294 | Escherichia coli | hydrogen peroxide | 1.77 × 10³ | 2.2 | 800 | pH 7, 23 °C | Open record: catalase-peroxidase KatG variant ΔP285/ΔG294, hydrogen peroxide |
| catalase-peroxidase KatGvariant ΔP285/ΔG294 | Escherichia coli | hydrogen peroxide | 332 | 199 | 1.8 | pH 5, 23 °C | Open record: catalase-peroxidase KatG variant ΔP285/ΔG294, hydrogen peroxide |