Values CatLog read from this paper
Quantitative functional characterization of conserved molecular interactions in the active site of mannitol 2-dehydrogenase
PMID 25752240doi:10.1002/pro.2669Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 31 records (79 values) from this paper: mannitol 2-dehydrogenase (pfMDH) from Pseudomonas fluorescens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| mannitol 2-dehydrogenase (pfMDH) | Pseudomonas fluorescens | D-arabitol | 6.59 | 55.85 | 0.118 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH), D-arabitol |
| mannitol 2-dehydrogenase (pfMDH) | Pseudomonas fluorescens | D-mannitol | 4.39 | 5.98 | 0.7342 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH), D-mannitol |
| mannitol 2-dehydrogenase (pfMDH) | Pseudomonas fluorescens | meso-erythritol | — | — | 0.00108 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH), meso-erythritol |
| mannitol 2-dehydrogenase (pfMDH)variant D230A | Pseudomonas fluorescens | D-arabitol | 0.0029 | 20.36 | 1.4 × 10⁻⁴ | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant D230A, D-arabitol |
| mannitol 2-dehydrogenase (pfMDH)variant D230A | Pseudomonas fluorescens | D-mannitol | 0.39 | 42.74 | 0.00908 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant D230A, D-mannitol |
| mannitol 2-dehydrogenase (pfMDH)variant E133A | Pseudomonas fluorescens | D-arabitol | 7.3 | 134.1 | 0.05448 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant E133A, D-arabitol |
| mannitol 2-dehydrogenase (pfMDH)variant E133A | Pseudomonas fluorescens | D-mannitol | 3.99 | 18.21 | 0.219 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant E133A, D-mannitol |
| mannitol 2-dehydrogenase (pfMDH)variant E133A | Pseudomonas fluorescens | meso-erythritol | — | — | 9.7 × 10⁻⁴ | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant E133A, meso-erythritol |
| mannitol 2-dehydrogenase (pfMDH)variant E133Q | Pseudomonas fluorescens | D-arabitol | 5.5 | 126.9 | 0.04332 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant E133Q, D-arabitol |
| mannitol 2-dehydrogenase (pfMDH)variant E133Q | Pseudomonas fluorescens | D-mannitol | 3.22 | 14.96 | 0.2151 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant E133Q, D-mannitol |
| mannitol 2-dehydrogenase (pfMDH)variant E133Q | Pseudomonas fluorescens | meso-erythritol | — | — | 8.8 × 10⁻⁴ | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant E133Q, meso-erythritol |
| mannitol 2-dehydrogenase (pfMDH)variant H303A | Pseudomonas fluorescens | D-arabitol | 0.29 | 145.1 | 0.002 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant H303A, D-arabitol |
| mannitol 2-dehydrogenase (pfMDH)variant H303A | Pseudomonas fluorescens | D-mannitol | 0.49 | 93.85 | 0.00522 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant H303A, D-mannitol |
| mannitol 2-dehydrogenase (pfMDH)variant H303A | Pseudomonas fluorescens | meso-erythritol | — | — | 2.9 × 10⁻⁵ | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant H303A, meso-erythritol |
| mannitol 2-dehydrogenase (pfMDH)variant H303A/R373A/K381A | Pseudomonas fluorescens | D-arabitol | 0.0017 | 33.15 | 5.1 × 10⁻⁵ | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant H303A/R373A/K381A, D-arabitol |
| mannitol 2-dehydrogenase (pfMDH)variant H303A/R373A/K381A | Pseudomonas fluorescens | D-mannitol | 0.001 | 8.15 | 1.6 × 10⁻⁴ | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant H303A/R373A/K381A, D-mannitol |
| mannitol 2-dehydrogenase (pfMDH)variant K381A | Pseudomonas fluorescens | D-arabitol | 0.37 | 176.9 | 0.00206 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant K381A, D-arabitol |
| mannitol 2-dehydrogenase (pfMDH)variant K381A | Pseudomonas fluorescens | D-mannitol | 0.19 | 23.89 | 0.00775 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant K381A, D-mannitol |
| mannitol 2-dehydrogenase (pfMDH)variant N191A | Pseudomonas fluorescens | D-arabitol | 0.044 | 125.5 | 3.5 × 10⁻⁴ | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant N191A, D-arabitol |
| mannitol 2-dehydrogenase (pfMDH)variant N191A | Pseudomonas fluorescens | D-mannitol | 0.0209 | 4.56 | 0.00458 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant N191A, D-mannitol |
| mannitol 2-dehydrogenase (pfMDH)variant N191A | Pseudomonas fluorescens | meso-erythritol | — | — | 2.1 × 10⁻⁶ | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant N191A, meso-erythritol |
| mannitol 2-dehydrogenase (pfMDH)variant N300A | Pseudomonas fluorescens | D-arabitol | 0.092 | 132.1 | 6.9 × 10⁻⁴ | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant N300A, D-arabitol |
| mannitol 2-dehydrogenase (pfMDH)variant N300A | Pseudomonas fluorescens | D-mannitol | 0.21 | 68.94 | 0.00311 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant N300A, D-mannitol |
| mannitol 2-dehydrogenase (pfMDH)variant N300A | Pseudomonas fluorescens | meso-erythritol | — | — | 6.5 × 10⁻⁶ | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant N300A, meso-erythritol |
| mannitol 2-dehydrogenase (pfMDH)variant N300D | Pseudomonas fluorescens | D-arabitol | 0.0013 | 94.82 | 1.4 × 10⁻⁵ | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant N300D, D-arabitol |
| mannitol 2-dehydrogenase (pfMDH)variant N300D | Pseudomonas fluorescens | D-mannitol | 8.3 × 10⁻⁴ | 20.42 | 4 × 10⁻⁵ | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant N300D, D-mannitol |
| mannitol 2-dehydrogenase (pfMDH)variant N300S | Pseudomonas fluorescens | D-arabitol | 0.06 | 126.5 | 4.7 × 10⁻⁴ | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant N300S, D-arabitol |
| mannitol 2-dehydrogenase (pfMDH)variant N300S | Pseudomonas fluorescens | D-mannitol | 0.096 | 49.95 | 0.00192 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant N300S, D-mannitol |
| mannitol 2-dehydrogenase (pfMDH)variant N300S | Pseudomonas fluorescens | meso-erythritol | — | — | 4.4 × 10⁻⁶ | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant N300S, meso-erythritol |
| mannitol 2-dehydrogenase (pfMDH)variant R373A | Pseudomonas fluorescens | D-arabitol | 0.034 | 144.5 | 2.4 × 10⁻⁴ | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant R373A, D-arabitol |
| mannitol 2-dehydrogenase (pfMDH)variant R373A | Pseudomonas fluorescens | D-mannitol | 0.018 | 17.49 | 0.00102 | pH 7.4 | Open record: mannitol 2-dehydrogenase (pfMDH) variant R373A, D-mannitol |