Values CatLog read from this paper
'Super-perfect' enzymes: Structural stabilities and activities of recombinant triose phosphate isomerases from Pyrococcus furiosus and Thermococcus onnurineus produced in Escherichia coli
PMID 25824038doi:10.1016/j.bbrc.2015.03.102Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 4 records (10 values) from this paper: triose phosphate isomerase (PfuTIM), triose phosphate isomerase (TonTIM) from Pyrococcus furiosus, Thermococcus onnurineus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| triose phosphate isomerase (PfuTIM) | Pyrococcus furiosus | D-glyceraldehyde 3-phosphate | 1.548 × 10⁶ | 1.219 | 1.269 × 10⁶ | pH 8, 25 °C | Open record: triose phosphate isomerase (PfuTIM), D-glyceraldehyde 3-phosphate |
| triose phosphate isomerase (PfuTIM) | Pyrococcus furiosus | D-glyceraldehyde 3-phosphate | 5.75 × 10⁷ | 0.6774 | 8.49 × 10⁷ calc. | pH 8, 90 °C | Open record: triose phosphate isomerase (PfuTIM), D-glyceraldehyde 3-phosphate |
| triose phosphate isomerase (TonTIM) | Thermococcus onnurineus | D-glyceraldehyde 3-phosphate | 1.43 × 10⁶ | 1.668 | 8.57 × 10⁵ | pH 8, 25 °C | Open record: triose phosphate isomerase (TonTIM), D-glyceraldehyde 3-phosphate |
| triose phosphate isomerase (TonTIM) | Thermococcus onnurineus | D-glyceraldehyde 3-phosphate | 2.5 × 10⁷ | 0.2076 | 1.2 × 10⁸ calc. | pH 8, 90 °C | Open record: triose phosphate isomerase (TonTIM), D-glyceraldehyde 3-phosphate |