Values CatLog read from this paper
Mesaconase Activity of Class I Fumarase Contributes to Mesaconate Utilization by Burkholderia xenovorans
PMID 26070669doi:10.1128/aem.00822-15Open in Browse
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CatLog read 8 records (14 values) from this paper: class I fumarase (iron-dependent fumarate hydratase), class II fumarase (iron-independent fumarate hydratase), mesaconase activity (attributed to class I fumarase) in cell extract from Burkholderia xenovorans.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| class I fumarase (iron-dependent fumarate hydratase) | Burkholderia xenovorans | (S)-citramalate ((S)-2-methylmalate) | — | 0.52 | 131 | pH 6.9, 30 °C | Open record: class I fumarase (iron-dependent fumarate hydratase), (S)-citramalate ((S)-2-methylmalate) |
| class I fumarase (iron-dependent fumarate hydratase) | Burkholderia xenovorans | (S)-malate | — | 0.28 | 398 | pH 6.9, 30 °C | Open record: class I fumarase (iron-dependent fumarate hydratase), (S)-malate |
| class I fumarase (iron-dependent fumarate hydratase) | Burkholderia xenovorans | fumarate | — | 0.1 | 2.8 × 10³ | pH 6.9, 30 °C | Open record: class I fumarase (iron-dependent fumarate hydratase), fumarate |
| class I fumarase (iron-dependent fumarate hydratase) | Burkholderia xenovorans | mesaconate (methylfumarate) | — | 0.03 | 3.6 × 10³ | pH 6.9, 30 °C | Open record: class I fumarase (iron-dependent fumarate hydratase), mesaconate (methylfumarate) |
| class II fumarase (iron-independent fumarate hydratase) | Burkholderia xenovorans | (S)-malate | — | 0.42 | 320 | pH 6.9, 30 °C | Open record: class II fumarase (iron-independent fumarate hydratase), (S)-malate |
| class II fumarase (iron-independent fumarate hydratase) | Burkholderia xenovorans | fumarate | — | 0.138 | 2.2 × 10³ | pH 6.9, 30 °C | Open record: class II fumarase (iron-independent fumarate hydratase), fumarate |
| mesaconase activity (attributed to class I fumarase) in cell extract | Burkholderia xenovorans | (S)-citramalate ((S)-2-methylmalate) | — | 0.47 | — | pH 6.9, 30 °C | Open record: mesaconase activity (attributed to class I fumarase) in cell extract, (S)-citramalate ((S)-2-methylmalate) |
| mesaconase activity (attributed to class I fumarase) in cell extract | Burkholderia xenovorans | mesaconate (methylfumarate) | — | 0.034 | — | pH 6.9, 30 °C | Open record: mesaconase activity (attributed to class I fumarase) in cell extract, mesaconate (methylfumarate) |