Values CatLog read from this paper

tRNA recognition by a bacterial tRNA Xm32 modification enzyme from the SPOUT methyltransferase superfamily

PMID 26202969doi:10.1093/nar/gkv745Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 15 records (30 values) from this paper: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 15 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.1.1.200

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ)	Escherichia coli	EctRNA^{Gln}_1(UUG)	0.02	0.00988	2.0 calc.	pH 9, 37 °C	Open record: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ), EctRNA^{Gln}_1(UUG)
TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ)	Escherichia coli	EctRNA^{Gln}_2(CUG)	0.0235	0.00579	4.06 calc.	pH 9, 37 °C	Open record: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ), EctRNA^{Gln}_2(CUG)
TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ)	Escherichia coli	EctRNA^{Ser}_1(UGA)	0.0383	0.01182	3.24 calc.	pH 9, 37 °C	Open record: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ), EctRNA^{Ser}_1(UGA)
TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ)	Escherichia coli	EctRNA^{Trp}_1(CCA)	0.0218	9.4 × 10⁻⁴	23 calc.	pH 9, 37 °C	Open record: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ), EctRNA^{Trp}_1(CCA)
TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ)	Escherichia coli	EctRNA_f^{Met}_1(CAU)	0.0253	6.7 × 10⁻⁴	38 calc.	pH 9, 37 °C	Open record: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ), EctRNA_f^{Met}_1(CAU)
TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ)	Escherichia coli	EctRNA_f^{Met}_1(CAU) dAcc1	0.0253	7.4 × 10⁻⁴	34 calc.	pH 9, 37 °C	Open record: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ), EctRNA_f^{Met}_1(CAU) dAcc1
TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ)	Escherichia coli	EctRNA_f^{Met}_1(CAU) dAcc3	0.024	8.8 × 10⁻⁴	27 calc.	pH 9, 37 °C	Open record: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ), EctRNA_f^{Met}_1(CAU) dAcc3
TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ)	Escherichia coli	EctRNA_f^{Met}_1(CAU) dAcc5	0.021	0.00137	15 calc.	pH 9, 37 °C	Open record: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ), EctRNA_f^{Met}_1(CAU) dAcc5
TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ)	Escherichia coli	EctRNA_f^{Met}_1(CAU) dCCA	0.0233	6.1 × 10⁻⁴	38 calc.	pH 9, 37 °C	Open record: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ), EctRNA_f^{Met}_1(CAU) dCCA
TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ)	Escherichia coli	EctRNA_f^{Met}_1(CAU) G18U/U55G	0.0223	0.00992	2.25 calc.	pH 9, 37 °C	Open record: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ), EctRNA_f^{Met}_1(CAU) G18U/U55G
TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ)	Escherichia coli	EctRNA_f^{Met}_2(CAU)	0.0237	8.1 × 10⁻⁴	29 calc.	pH 9, 37 °C	Open record: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ), EctRNA_f^{Met}_2(CAU)
TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ)variant K211A	Escherichia coli	EctRNA_f^{Met}_1(CAU)	0.0193	0.01376	1.41 calc.	pH 9, 37 °C	Open record: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ) variant K211A, EctRNA_f^{Met}_1(CAU)
TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ)variant R115A	Escherichia coli	EctRNA_f^{Met}_1(CAU)	0.024	6.3 × 10⁻⁴	38 calc.	pH 9, 37 °C	Open record: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ) variant R115A, EctRNA_f^{Met}_1(CAU)
TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ)variant R214A	Escherichia coli	EctRNA_f^{Met}_1(CAU)	0.0303	0.01231	2.46 calc.	pH 9, 37 °C	Open record: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ) variant R214A, EctRNA_f^{Met}_1(CAU)
TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ)variant R218A	Escherichia coli	EctRNA_f^{Met}_1(CAU)	0.0185	0.00543	3.41 calc.	pH 9, 37 °C	Open record: TrmJ (tRNA Xm32 2'-O-methyltransferase, EcTrmJ) variant R218A, EctRNA_f^{Met}_1(CAU)