Values CatLog read from this paper
Kinetic Analysis of a Globin-Coupled Histidine Kinase, AfGcHK: Effects of the Heme Iron Complex, Response Regulator, and Metal Cations on Autophosphorylation Activity
PMID 26212354doi:10.1021/acs.biochem.5b00517Open in Browse
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CatLog read 28 records (36 values) from this paper: AfGcHK (globin-coupled histidine kinase) from Anaeromyxobacter sp. Fw109-5.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; heme Fe(III)-OH− (native form); apparent Km | — | 0.0189 | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; heme Fe(III)-OH− (native form); apparent kcat | 0.018 | — | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; heme Fe(III)-OH− (native form); apparent kcat/Km | — | — | 1 | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; apparent Km; Table 1 | — | 0.0354 | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; apparent kcat; Table 1 | 0.02 | — | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; apparent kcat/Km; Table 1 | — | — | 0.6 | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; apparent Km; Table 1 | — | 0.0238 | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; apparent kcat; Table 1 | 0.018 | — | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; apparent kcat/Km; Table 1 | — | — | 0.8 | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; heme Fe(II)-O2 complex; apparent Km; Table 1 | — | 0.023 | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; heme Fe(II)-O2 complex; apparent kcat; Table 1 | 0.017 | — | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; heme Fe(II)-O2 complex; apparent kcat/Km | — | — | 0.7 | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; apparent Km; Table 1 | — | 0.357 | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; apparent kcat; Table 1 | 0.017 | — | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; apparent kcat/Km; Table 1 | — | — | 0.05 | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; apparent Km; Table 1 | — | 0.078 | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; apparent kcat; Table 1 | 0.0067 | — | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; apparent kcat/Km; Table 1 | — | — | 0.09 | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; heme Fe(III)-OH− (native form); apparent Km | — | 0.0477 | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; heme Fe(III)-OH− (native form); apparent kcat | 0.017 | — | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MgCl2; heme Fe(III)-OH− (native form); apparent kcat/Km | — | — | 0.3 | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphateno MgCl2 (no divalent metal); heme Fe(III)-OH− (native form) | — | 0.152 | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphateno MgCl2 (no divalent metal); heme Fe(III)-OH− (native form) | 0.0027 | — | — | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphateno MgCl2 (no divalent metal); heme Fe(III)-OH− (native form) | — | — | 0.02 | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM MnCl2 instead of MgCl2; heme Fe(III)-OH− (native form) | 0.022 | 0.0932 | 0.233 | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate5 mM CaCl2 instead of MgCl2; heme Fe(III)-OH− (native form) | 0.012 | 0.4039 | 0.03 | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase) | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphateheme Fe(III)-OH− (native form); apparent kcat; apparent Km | 0.0033 | 0.0559 | 0.06 | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase), adenosine 5'-triphosphate |
| AfGcHK (globin-coupled histidine kinase)variant H99A | Anaeromyxobacter sp. Fw109-5 | adenosine 5'-triphosphate | 0.005 | 0.0336 | 0.12 | pH 8, 20 °C | Open record: AfGcHK (globin-coupled histidine kinase) variant H99A, adenosine 5'-triphosphate |