CatLogenzyme kinetics catalog

Values CatLog read from this paper

Variants of Phosphotriesterase for the Enhanced Detoxification of the Chemical Warfare Agent VR

PMID 26274608doi:10.1021/acs.biochem.5b00629Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 122 records (280 values) from this paper: phosphotriesterase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.1.8.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
phosphotriesterase—O,O-diethyl VR0.2010.380.56pH 8, 30 °COpen
phosphotriesterase—O,O-diethyl VX1.10.871.2pH 8, 30 °COpen
phosphotriesterase—O,O-dimethyl VX0.0211.10.019pH 8, 30 °COpen
phosphotriesterase—O-methyl VR——0.068pH 8, 30 °COpen
phosphotriesterase—paraoxon2.23 × 10³0.0812.8 × 10⁴pH 9, 30 °COpen
phosphotriesterase—RP-p-acetophenyl VR841.749pH 9, 30 °COpen
phosphotriesterase—RP-S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——0.11pH 8Open
phosphotriesterase—S-(2-(diisopropylamino)ethyl) O-ethyl methylphosphonothioate——0.084pH 8Open
phosphotriesterase—SP-p-acetophenyl VR254.56pH 9, 30 °COpen
phosphotriesterase—SP-S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——0.0043pH 8Open
phosphotriesterasevariant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L—O,O-diethyl VR3.10.368.7pH 8, 30 °COpen
phosphotriesterasevariant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L—O,O-diethyl VX220.7331pH 8, 30 °COpen
phosphotriesterasevariant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L—O,O-dimethyl VX5.10.915.5pH 8, 30 °COpen
phosphotriesterasevariant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L—O-methyl VR——0.51pH 8, 30 °COpen
phosphotriesterasevariant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L—paraoxon1160.0081.5 × 10⁴pH 9, 30 °COpen
phosphotriesterasevariant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L—RP-p-acetophenyl VR550.16340pH 9, 30 °COpen
phosphotriesterasevariant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L—RP-S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——2.4pH 8Open
phosphotriesterasevariant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L—S-(2-(diisopropylamino)ethyl) O-ethyl methylphosphonothioate——110pH 8Open
phosphotriesterasevariant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L—S-(2-(diisopropylamino)ethyl) O-ethyl methylphosphonothioate——43pH 8Open
phosphotriesterasevariant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L—SP-p-acetophenyl VR291.717pH 9, 30 °COpen
phosphotriesterasevariant CVQF-I106G/L308S—O,O-diethyl VX——0.31pH 8, 30 °COpen
phosphotriesterasevariant CVQF-I106G/L308S—paraoxon480.0251.9 × 10³pH 9, 30 °COpen
phosphotriesterasevariant CVQFL C106I—RP-p-acetophenyl VR500.15330pH 9, 30 °COpen
phosphotriesterasevariant CVQFL C106I—SP-p-acetophenyl VR141.59.5pH 9, 30 °COpen
phosphotriesterasevariant CVQFL-C106I—O,O-diethyl VX140.6522pH 8, 30 °COpen
phosphotriesterasevariant CVQFL-C106I—paraoxon660.00541.2 × 10⁴pH 9, 30 °COpen
phosphotriesterasevariant CVQFL-C106I/L308S—O,O-diethyl VX19119pH 8, 30 °COpen
phosphotriesterasevariant CVQFL-C106I/L308S—paraoxon1080.0111 × 10⁴pH 9, 30 °COpen
phosphotriesterasevariant CVQFL-I106G—O,O-diethyl VX312.811pH 8, 30 °COpen
phosphotriesterasevariant CVQFL-I106G—paraoxon4560.0222 × 10⁴pH 9, 30 °COpen
phosphotriesterasevariant CVQFL-I106G—RP-p-acetophenyl VR1741.4120pH 9, 30 °COpen
phosphotriesterasevariant CVQFL-I106G—SP-p-acetophenyl VR360.2180pH 9, 30 °COpen
phosphotriesterasevariant CVQFL-I106G/L308S—RP-p-acetophenyl VR100424pH 9, 30 °COpen
phosphotriesterasevariant CVQFL-I106G/L308S—SP-p-acetophenyl VR400.24160pH 9, 30 °COpen
phosphotriesterasevariant CVQFL-L308S—O,O-diethyl VX133.24.1pH 8, 30 °COpen
phosphotriesterasevariant CVQFL-L308S—paraoxon330.00674.8 × 10³pH 9, 30 °COpen
phosphotriesterasevariant CVQFL-L308S—RP-p-acetophenyl VR122260pH 9, 30 °COpen
phosphotriesterasevariant CVQFL-L308S—SP-p-acetophenyl VR8.10.1942pH 9, 30 °COpen
phosphotriesterasevariant H254Q/H257F—O,O-diethyl VR0.820.372.2pH 8, 30 °COpen
phosphotriesterasevariant H254Q/H257F—O,O-diethyl VX6.11.44.2pH 8, 30 °COpen
phosphotriesterasevariant H254Q/H257F—O,O-dimethyl VX0.210.80.26pH 8, 30 °COpen
phosphotriesterasevariant H254Q/H257F—O-methyl VR0.151.70.071pH 8, 30 °COpen
phosphotriesterasevariant H254Q/H257F—paraoxon410.00537.7 × 10³pH 9, 30 °COpen
phosphotriesterasevariant H254Q/H257F—RP-p-acetophenyl VR570.36160pH 9, 30 °COpen
phosphotriesterasevariant H254Q/H257F—RP-S-(2-(diisopropylamino)ethyl) O-ethyl methylphosphonothioate——1.5pH 8Open
phosphotriesterasevariant H254Q/H257F—S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——0.48pH 8Open
phosphotriesterasevariant H254Q/H257F—S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——0.079pH 8Open
phosphotriesterasevariant H254Q/H257F—SP-p-acetophenyl VR8.70.03290pH 9, 30 °COpen
phosphotriesterasevariant H254Q/H257F—SP-S-(2-(diisopropylamino)ethyl) O-ethyl methylphosphonothioate——17pH 8Open
phosphotriesterasevariant I106C/F132V/H254Q/H257F/S308L—O,O-diethyl VR1.490.197.8pH 8, 30 °COpen
phosphotriesterasevariant I106C/F132V/H254Q/H257F/S308L—O,O-diethyl VX160.7621pH 8, 30 °COpen
phosphotriesterasevariant I106C/F132V/H254Q/H257F/S308L—O,O-dimethyl VX173.74.4pH 8, 30 °COpen
phosphotriesterasevariant I106C/F132V/H254Q/H257F/S308L—O-methyl VR——0.21pH 8, 30 °COpen
phosphotriesterasevariant I106C/F132V/H254Q/H257F/S308L—paraoxon380.00566.8 × 10³pH 9, 30 °COpen
phosphotriesterasevariant I106C/F132V/H254Q/H257F/S308L—RP-p-acetophenyl VR460.18260pH 9, 30 °COpen
phosphotriesterasevariant I106C/F132V/H254Q/H257F/S308L—S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——5.5pH 8Open
phosphotriesterasevariant I106C/F132V/H254Q/H257F/S308L—S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——0.89pH 8Open
phosphotriesterasevariant I106C/F132V/H254Q/H257F/S308L—S-(2-(diisopropylamino)ethyl) O-ethyl methylphosphonothioate——100pH 8Open
phosphotriesterasevariant I106C/F132V/H254Q/H257F/S308L—SP-p-acetophenyl VR210.19110pH 9, 30 °COpen
phosphotriesterasevariant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L—O,O-diethyl VR6.80.4415pH 8, 30 °COpen
phosphotriesterasevariant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L—O,O-diethyl VX510.685pH 8, 30 °COpen
phosphotriesterasevariant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L—O,O-dimethyl VX282.113pH 8, 30 °COpen
phosphotriesterasevariant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L—O-methyl VR11.50.58pH 8, 30 °COpen
phosphotriesterasevariant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L—paraoxon850.00721.2 × 10⁴pH 9, 30 °COpen
phosphotriesterasevariant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L—RP-p-acetophenyl VR1010.62160pH 9, 30 °COpen
phosphotriesterasevariant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L—S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——2.2pH 8Open
phosphotriesterasevariant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L—S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——0.21pH 8Open
phosphotriesterasevariant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L—S-(2-(diisopropylamino)ethyl) O-ethyl methylphosphonothioate——830pH 8Open
phosphotriesterasevariant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L—S-(2-(diisopropylamino)ethyl) O-ethyl methylphosphonothioate——220pH 8Open
phosphotriesterasevariant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L—SP-p-acetophenyl VR560.5110pH 9, 30 °COpen
phosphotriesterasevariant L7ep-3a I106G—O,O-diethyl VR460.67pH 8, 30 °COpen
phosphotriesterasevariant L7ep-3a I106G—O,O-diethyl VX——7.7pH 8, 30 °COpen
phosphotriesterasevariant L7ep-3a I106G—O,O-dimethyl VX——1.4pH 8, 30 °COpen
phosphotriesterasevariant L7ep-3a I106G—O-methyl VR——0.27pH 8, 30 °COpen
phosphotriesterasevariant L7ep-3a I106G—paraoxon5450.0331.67 × 10⁴pH 9, 30 °COpen
phosphotriesterasevariant L7ep-3a I106G—RP-p-acetophenyl VR580.7182pH 9, 30 °COpen
phosphotriesterasevariant L7ep-3a I106G—S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——2.6pH 8Open
phosphotriesterasevariant L7ep-3a I106G—S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——0.69pH 8Open
phosphotriesterasevariant L7ep-3a I106G—S-(2-(diisopropylamino)ethyl) O-ethyl methylphosphonothioate——20pH 8Open
phosphotriesterasevariant L7ep-3a I106G—S-(2-(diisopropylamino)ethyl) O-ethyl methylphosphonothioate——6.2pH 8Open
phosphotriesterasevariant L7ep-3a I106G—SP-p-acetophenyl VR1660.31530pH 9, 30 °COpen
phosphotriesterasevariant L7ep-3a Y309F—O,O-diethyl VX361.230pH 8, 30 °COpen
phosphotriesterasevariant L7ep-3a Y309F—paraoxon1310.00921.43 × 10⁴pH 9, 30 °COpen
phosphotriesterasevariant VRN-VQF-I106C/L308S—RP-p-acetophenyl VR1601.7100pH 9, 30 °COpen
phosphotriesterasevariant VRN-VQF-I106C/L308S—SP-p-acetophenyl VR511.534pH 9, 30 °COpen
phosphotriesterasevariant VRN-VQF-L308S—O,O-diethyl VR1.940.219.2pH 8, 30 °COpen
phosphotriesterasevariant VRN-VQF-L308S—O,O-dimethyl VX31.81.6pH 8, 30 °COpen
phosphotriesterasevariant VRN-VQF-L308S—O-methyl VR——0.14pH 8, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106C—O,O-diethyl VX230.828pH 8, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106C—paraoxon1030.00531.9 × 10⁴pH 9, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106C—RP-p-acetophenyl VR720.23320pH 9, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106C—SP-p-acetophenyl VR330.23140pH 9, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106C/L308S—O,O-diethyl VX8.61.65.4pH 8, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106C/L308S—paraoxon350.00369.7 × 10³pH 9, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106G—O,O-diethyl VR0.470.80.59pH 8, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106G—O,O-diethyl VX——5pH 8, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106G—O,O-dimethyl VX0.681.60.44pH 8, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106G—O-methyl VR——0.33pH 8, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106G—paraoxon4460.0212.1 × 10⁴pH 9, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106G—RP-p-acetophenyl VR560.41140pH 9, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106G—S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——2pH 8Open
phosphotriesterasevariant VRN-VQFL-I106G—S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——0.41pH 8Open
phosphotriesterasevariant VRN-VQFL-I106G—S-(2-(diisopropylamino)ethyl) O-ethyl methylphosphonothioate——6.6pH 8Open
phosphotriesterasevariant VRN-VQFL-I106G—SP-p-acetophenyl VR1590.18900pH 9, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106G/L308S—O,O-diethyl VR0.1150.970.12pH 8, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106G/L308S—O,O-diethyl VX——0.41pH 8, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106G/L308S—O,O-dimethyl VX0.31.150.26pH 8, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106G/L308S—O-methyl VR——0.19pH 8, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106G/L308S—paraoxon580.00926.2 × 10³pH 9, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106G/L308S—RP-p-acetophenyl VR452.122pH 9, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106G/L308S—RP-S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——0.067pH 8Open
phosphotriesterasevariant VRN-VQFL-I106G/L308S—S-(2-(diisopropylamino)ethyl) O-ethyl methylphosphonothioate——4.9pH 8Open
phosphotriesterasevariant VRN-VQFL-I106G/L308S—S-(2-(diisopropylamino)ethyl) O-ethyl methylphosphonothioate——1.7pH 8Open
phosphotriesterasevariant VRN-VQFL-I106G/L308S—SP-p-acetophenyl VR530.18300pH 9, 30 °COpen
phosphotriesterasevariant VRN-VQFL-I106G/L308S—SP-S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——2.1pH 8Open
phosphotriesterasevariant VRN-VQFL-L308S—O,O-diethyl VX5.10.3515pH 8, 30 °COpen
phosphotriesterasevariant VRN-VQFL-L308S—paraoxon150.00178.8 × 10³pH 9, 30 °COpen
phosphotriesterasevariant VRN-VQFL-L308S—RP-p-acetophenyl VR170.5232pH 9, 30 °COpen
phosphotriesterasevariant VRN-VQFL-L308S—S-(2-(diethylamino)ethyl) O-isobutyl methylphosphonothioate——0.27pH 8Open
phosphotriesterasevariant VRN-VQFL-L308S—S-(2-(diisopropylamino)ethyl) O-ethyl methylphosphonothioate——62pH 8Open
phosphotriesterasevariant VRN-VQFL-L308S—S-(2-(diisopropylamino)ethyl) O-ethyl methylphosphonothioate——8.9pH 8Open
phosphotriesterasevariant VRN-VQFL-L308S—SP-p-acetophenyl VR50.1339pH 9, 30 °COpen