Values CatLog read from this paper
Structural Basis for Specificity and Flexibility in a Plant 4-Coumarate:CoA Ligase
PMID 26412334doi:10.1016/j.str.2015.08.012Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 21 records (63 values) from this paper: Nt4CL2 (4-coumarate:CoA ligase isoform 2) from Nicotiana tabacum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Nt4CL2 (4-coumarate:CoA ligase isoform 2) | Nicotiana tabacum | 4-coumaric acid | 3.978 | 0.0015 | 2652 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2), 4-coumaric acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2) | Nicotiana tabacum | caffeic acid | 2.652 | 0.001 | 2652 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2), caffeic acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2) | Nicotiana tabacum | ferulic acid | 3.576 | 0.0029 | 1233 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2), ferulic acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2) | Nicotiana tabacum | sinapinic acid | 0.018 | 1.322 | 0.01362 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2), sinapinic acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant H237A | Nicotiana tabacum | 4-coumaric acid | 1.98 | 0.1434 | 13.81 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant H237A, 4-coumaric acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant K197A | Nicotiana tabacum | 4-coumaric acid | 7.11 | 0.0139 | 511.5 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant K197A, 4-coumaric acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant K443A | Nicotiana tabacum | 4-coumaric acid | 1.47 | 9 × 10⁻⁴ | 1633 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant K443A, 4-coumaric acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant M344A | Nicotiana tabacum | 4-coumaric acid | 9.144 | 0.5207 | 175.6 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant M344A, 4-coumaric acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant R435A | Nicotiana tabacum | 4-coumaric acid | 3.036 | 0.6149 | 4.937 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant R435A, 4-coumaric acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant T193A | Nicotiana tabacum | 4-coumaric acid | 0.996 | 0.0029 | 343.4 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant T193A, 4-coumaric acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant T336A | Nicotiana tabacum | 4-coumaric acid | 0.282 | 0.0051 | 55.29 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant T336A, 4-coumaric acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant V341G | Nicotiana tabacum | 4-coumaric acid | 2.574 | 0.0043 | 598.6 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant V341G, 4-coumaric acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant V341G | Nicotiana tabacum | caffeic acid | 2.034 | 0.0043 | 473 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant V341G, caffeic acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant V341G | Nicotiana tabacum | ferulic acid | 2.478 | 0.0047 | 527.2 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant V341G, ferulic acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant V341G | Nicotiana tabacum | sinapinic acid | 0.036 | 1.230 | 0.02926 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant V341G, sinapinic acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant Y239A | Nicotiana tabacum | 4-coumaric acid | 0.996 | 0.2281 | 4.367 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant Y239A, 4-coumaric acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant Y239F | Nicotiana tabacum | 4-coumaric acid | 4.47 | 0.0069 | 647.8 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant Y239F, 4-coumaric acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant ΔV341 | Nicotiana tabacum | 4-coumaric acid | 2.406 | 0.0137 | 175.6 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant ΔV341, 4-coumaric acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant ΔV341 | Nicotiana tabacum | caffeic acid | 1.272 | 0.0154 | 82.6 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant ΔV341, caffeic acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant ΔV341 | Nicotiana tabacum | ferulic acid | 2.286 | 0.0311 | 73.51 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant ΔV341, ferulic acid |
| Nt4CL2 (4-coumarate:CoA ligase isoform 2)variant ΔV341 | Nicotiana tabacum | sinapinic acid | 0.774 | 0.3128 | 2.474 | pH 7.5, 25 °C | Open record: Nt4CL2 (4-coumarate:CoA ligase isoform 2) variant ΔV341, sinapinic acid |