CatLogenzyme kinetics catalog

Values CatLog read from this paper

Role of Loop-Clamping Side Chains in Catalysis by Triosephosphate Isomerase

PMID 26570983doi:10.1021/jacs.5b09328Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 35 records (53 values) from this paper: triosephosphate isomerase from Saccharomyces cerevisiae.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 5.3.1.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
triosephosphate isomeraseSaccharomyces cerevisiaedihydroxyacetone phosphate8601.7510pH 7.5, 25 °COpen
triosephosphate isomeraseSaccharomyces cerevisiaeglycolaldehyde——0.04825 °COpen
triosephosphate isomerasevariant S211ASaccharomyces cerevisiaedihydroxyacetone phosphate——10pH 7.5, 25 °COpen
triosephosphate isomerasevariant S211GSaccharomyces cerevisiaeD-glyceraldehyde 3-phosphateparameters calculated using the total concentration of GAP7.5 × 10³1.84.2 × 10³pH 7.5, 25 °COpen
triosephosphate isomerasevariant S211GSaccharomyces cerevisiaeD-glyceraldehyde 3-phosphate——1.05 × 10⁵pH 7.5, 25 °COpen
triosephosphate isomerasevariant S211GSaccharomyces cerevisiaedihydroxyacetone phosphate8103.2250pH 7.5, 25 °COpen
triosephosphate isomerasevariant S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole, 20% free base); Table 2——1.3 × 10⁻⁴25 °COpen
triosephosphate isomerasevariant S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole, 20% free base); approximate kcat/Km——0.2125 °COpen
triosephosphate isomerasevariant S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); 2.5 mM HPO3 2- (phosphite dianion)——0.0070225 °COpen
triosephosphate isomerasevariant S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); 5.0 mM HPO3 2- (phosphite dianion)——0.015125 °COpen
triosephosphate isomerasevariant S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); 7.5 mM HPO3 2- (phosphite dianion)——0.021125 °COpen
triosephosphate isomerasevariant S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); 10 mM HPO3 2- (phosphite dianion)——0.028925 °COpen
triosephosphate isomerasevariant S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); Supplementary Table S1——0.031825 °COpen
triosephosphate isomerasevariant S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); 15 mM HPO3 2- (phosphite dianion)——0.046325 °COpen
triosephosphate isomerasevariant S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); Supplementary Table S1——0.044625 °COpen
triosephosphate isomerasevariant S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); Supplementary Table S1——0.04825 °COpen
triosephosphate isomerasevariant Y208ASaccharomyces cerevisiaedihydroxyacetone phosphate2101712pH 7.5, 25 °COpen
triosephosphate isomerasevariant Y208FSaccharomyces cerevisiaedihydroxyacetone phosphate4.5170.26pH 7.5, 25 °COpen
triosephosphate isomerasevariant Y208SSaccharomyces cerevisiaedihydroxyacetone phosphate2502510pH 7.5, 25 °COpen
triosephosphate isomerasevariant Y208SSaccharomyces cerevisiaeglycolaldehyde——0.004325 °COpen
triosephosphate isomerasevariant Y208TSaccharomyces cerevisiaedihydroxyacetone phosphate5801153pH 7.5, 25 °COpen
triosephosphate isomerasevariant Y208TSaccharomyces cerevisiaeglycolaldehyde——0.02125 °COpen
triosephosphate isomerasevariant Y208T/S211GSaccharomyces cerevisiaeD-glyceraldehyde 3-phosphateparameters calculated using the total concentration of GAP5200.71730pH 7.5, 25 °COpen
triosephosphate isomerasevariant Y208T/S211GSaccharomyces cerevisiaeD-glyceraldehyde 3-phosphate——1.8 × 10⁴pH 7.5, 25 °COpen
triosephosphate isomerasevariant Y208T/S211GSaccharomyces cerevisiaedihydroxyacetone phosphate135434pH 7.5, 25 °COpen
triosephosphate isomerasevariant Y208T/S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole, 20% free base); Table 2——1.2 × 10⁻⁴25 °COpen
triosephosphate isomerasevariant Y208T/S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole, 20% free base); Table 2——0.01825 °COpen
triosephosphate isomerasevariant Y208T/S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); 2.5 mM HPO3 2- (phosphite dianion)——0.0027825 °COpen
triosephosphate isomerasevariant Y208T/S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); 5.0 mM HPO3 2- (phosphite dianion)——0.0071925 °COpen
triosephosphate isomerasevariant Y208T/S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); 7.5 mM HPO3 2- (phosphite dianion)——0.0087625 °COpen
triosephosphate isomerasevariant Y208T/S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); 10 mM HPO3 2- (phosphite dianion)——0.009525 °COpen
triosephosphate isomerasevariant Y208T/S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); Supplementary Table S2——0.011725 °COpen
triosephosphate isomerasevariant Y208T/S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); 15 mM HPO3 2- (phosphite dianion)——0.012825 °COpen
triosephosphate isomerasevariant Y208T/S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); Supplementary Table S2——0.013725 °COpen
triosephosphate isomerasevariant Y208T/S211GSaccharomyces cerevisiaeglycolaldehydepD 7.0 (20 mM imidazole); Supplementary Table S2——0.015125 °COpen