Values CatLog read from this paper
The crystal structure of an inverting glycoside hydrolase family 9 exo-β-D-glucosaminidase and the design of glycosynthase
PMID 26621872doi:10.1042/bj20150966Open in Browse
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CatLog read 9 records (19 values) from this paper: exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520) from Photobacterium profundum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520) | Photobacterium profundum | alpha-D-glucopyranosyl fluoride | — | — | 0.0078 | pH 7, 37 °C | Open record: exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520), alpha-D-glucopyranosyl fluoride |
| exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520) | Photobacterium profundum | alpha-D-glucosaminyl fluoride | — | — | 0.73 | pH 7, 37 °C | Open record: exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520), alpha-D-glucosaminyl fluoride |
| exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520) | Photobacterium profundum | beta-D-glucosaminyl-(1->4)-D-glucosamine | 87 | 1.8 | 49 | pH 7, 37 °C | Open record: exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520), beta-D-glucosaminyl-(1->4)-D-glucosamine |
| exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520)variant D139A | Photobacterium profundum | alpha-D-glucosaminyl fluoride | — | — | 0.0019 | pH 7, 37 °C | Open record: exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520) variant D139A, alpha-D-glucosaminyl fluoride |
| exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520)variant D139A | Photobacterium profundum | beta-D-glucosaminyl-(1->4)-D-glucosamine | 0.0047 | 7.7 | 6.2 × 10⁻⁴ | pH 7, 37 °C | Open record: exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520) variant D139A, beta-D-glucosaminyl-(1->4)-D-glucosamine |
| exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520)variant D139E | Photobacterium profundum | alpha-D-glucosaminyl fluoride | 0.073 | 3.5 | 0.021 | pH 7, 37 °C | Open record: exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520) variant D139E, alpha-D-glucosaminyl fluoride |
| exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520)variant D139E | Photobacterium profundum | beta-D-glucosaminyl-(1->4)-D-glucosamine | 0.071 | 1.8 | 0.04 | pH 7, 37 °C | Open record: exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520) variant D139E, beta-D-glucosaminyl-(1->4)-D-glucosamine |
| exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520)variant D139S | Photobacterium profundum | alpha-D-glucosaminyl fluoride | — | — | 0.0031 | pH 7, 37 °C | Open record: exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520) variant D139S, alpha-D-glucosaminyl fluoride |
| exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520)variant D139S | Photobacterium profundum | beta-D-glucosaminyl-(1->4)-D-glucosamine | 0.54 | 15 | 0.036 | pH 7, 37 °C | Open record: exo-beta-D-glucosaminidase PpGlcNase (GH9, PBPRA0520) variant D139S, beta-D-glucosaminyl-(1->4)-D-glucosamine |