Values CatLog read from this paper
Substrate and pH-Dependent Kinetic Profile of 3-Mercaptopropionate Dioxygenase from Pseudomonas aeruginosa
PMID 26878277doi:10.1021/acs.biochem.5b01203Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 23 records (67 values) from this paper: 3-mercaptopropionate dioxygenase from Pseudomonas aeruginosa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | 3-mercaptopropionic acid | 0.11 | 4 | 0.03 | pH 4.7, 37 °C | Open record: 3-mercaptopropionate dioxygenase, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | 3-mercaptopropionic acid | 0.14 | 4 | 0.04 | pH 5.1, 37 °C | Open record: 3-mercaptopropionate dioxygenase, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | 3-mercaptopropionic acid | 0.22 | 5 | 0.05 | pH 5.5, 37 °C | Open record: 3-mercaptopropionate dioxygenase, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | 3-mercaptopropionic acid | 0.23 | 0.9 | 0.26 | pH 6.1, 37 °C | Open record: 3-mercaptopropionate dioxygenase, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | 3-mercaptopropionic acid100 mM PIPES buffer | 0.19 | 1 | 0.2 | pH 6.5, 37 °C | Open record: 3-mercaptopropionate dioxygenase, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | 3-mercaptopropionic acid100 mM sodium phosphate buffer | 0.2 | 1.5 | 0.13 | pH 6.5, 37 °C | Open record: 3-mercaptopropionate dioxygenase, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | 3-mercaptopropionic acid100 mM PIPES buffer | 0.13 | 0.5 | 0.27 | pH 7, 37 °C | Open record: 3-mercaptopropionate dioxygenase, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | 3-mercaptopropionic acid100 mM sodium phosphate buffer | 0.11 | 0.9 | 0.13 | pH 7, 37 °C | Open record: 3-mercaptopropionate dioxygenase, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | 3-mercaptopropionic acid100 mM PIPES buffer | 0.1 | 0.8 | 0.12 | pH 7.5, 37 °C | Open record: 3-mercaptopropionate dioxygenase, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | 3-mercaptopropionic acid100 mM sodium phosphate buffer | 0.1 | 0.7 | 0.13 | pH 7.5, 37 °C | Open record: 3-mercaptopropionate dioxygenase, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | 3-mercaptopropionic acid100 mM Tris buffer | 0.1 | 0.6 | 0.18 | pH 7.5, 37 °C | Open record: 3-mercaptopropionate dioxygenase, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | 3-mercaptopropionic acid100 mM sodium phosphate buffer | 0.03 | 0.5 | 0.06 | pH 8, 37 °C | Open record: 3-mercaptopropionate dioxygenase, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | 3-mercaptopropionic acid100 mM Tris buffer | 0.05 | 0.7 | 0.07 | pH 8, 37 °C | Open record: 3-mercaptopropionate dioxygenase, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | 3-mercaptopropionic acid100 mM Tris buffer | 0.03 | 1.4 | 0.018 | pH 8.5, 37 °C | Open record: 3-mercaptopropionate dioxygenase, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | 3-mercaptopropionic acid100 mM AMPSO buffer | 0.02 | 1.1 | 0.018 | pH 8.5, 37 °C | Open record: 3-mercaptopropionate dioxygenase, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | L-cysteine | 0.011 | 16 | 7 × 10⁻⁴ | pH 6.1, 37 °C | Open record: 3-mercaptopropionate dioxygenase, L-cysteine |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | L-cysteine | 0.022 | 16 | 0.0014 | pH 6.5, 37 °C | Open record: 3-mercaptopropionate dioxygenase, L-cysteine |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | L-cysteine | 0.033 | 15 | 0.0022 | pH 7, 37 °C | Open record: 3-mercaptopropionate dioxygenase, L-cysteine |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | L-cysteine100 mM PIPES buffer | 0.019 | 13 | 0.0015 | pH 7.5, 37 °C | Open record: 3-mercaptopropionate dioxygenase, L-cysteine |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | L-cysteine100 mM sodium phosphate buffer | 0.021 | 13 | 0.0016 | pH 7.5, 37 °C | Open record: 3-mercaptopropionate dioxygenase, L-cysteine |
| 3-mercaptopropionate dioxygenase | Pseudomonas aeruginosa | L-cysteine | 0.013 | 12 | 0.0011 | pH 8, 37 °C | Open record: 3-mercaptopropionate dioxygenase, L-cysteine |
| 3-mercaptopropionate dioxygenasevariant G95C | Pseudomonas aeruginosa | 3-mercaptopropionic acid | 0.007 | 0.9 | 0.0078 calc. | pH 6.5, 37 °C | Open record: 3-mercaptopropionate dioxygenase variant G95C, 3-mercaptopropionic acid |
| 3-mercaptopropionate dioxygenasevariant G95C | Pseudomonas aeruginosa | L-cysteine | 0.003 | 22 | 1.4 × 10⁻⁴ calc. | pH 6.5, 37 °C | Open record: 3-mercaptopropionate dioxygenase variant G95C, L-cysteine |