Values CatLog read from this paper
Crystal structure and identification of a key amino acid for glucose tolerance, substrate specificity, and transglycosylation activity of metagenomic β-glucosidase Td2F2
PMID 27092463doi:10.1111/febs.13743Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 48 records (72 values) from this paper: β-glucosidase Td2F2.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| β-glucosidase Td2F2 | — | 4-nitrophenyl beta-D-fucopyranoside | 16 | 0.14 | 115 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2, 4-nitrophenyl beta-D-fucopyranoside |
| β-glucosidase Td2F2 | — | 4-nitrophenyl beta-D-galactopyranoside | 30.8 | 3.61 | 8.52 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2, 4-nitrophenyl beta-D-galactopyranoside |
| β-glucosidase Td2F2 | — | 4-nitrophenyl beta-D-glucopyranoside | — | 0.21 | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2 | — | 4-nitrophenyl beta-D-glucopyranoside | 6.94 | — | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2 | — | 4-nitrophenyl beta-D-glucopyranoside | — | — | 32.3 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2 | — | 4-nitrophenyl beta-D-glucopyranoside | — | 2.65 | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2 | — | 4-nitrophenyl beta-D-glucopyranoside | 69 | — | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2 | — | 4-nitrophenyl beta-D-glucopyranoside | — | — | 26.1 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2DGR (Gly inserted between Asp324 and Arg325) | — | 4-nitrophenyl beta-D-fucopyranoside | 7.44 | 0.08 | 92.8 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 DGR (Gly inserted between Asp324 and Arg325), 4-nitrophenyl beta-D-fucopyranoside |
| β-glucosidase Td2F2DGR (Gly inserted between Asp324 and Arg325) | — | 4-nitrophenyl beta-D-galactopyranoside | 7.62 | 2.2 | 3.46 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 DGR (Gly inserted between Asp324 and Arg325), 4-nitrophenyl beta-D-galactopyranoside |
| β-glucosidase Td2F2DGR (Gly inserted between Asp324 and Arg325) | — | 4-nitrophenyl beta-D-glucopyranoside | — | 0.037 | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 DGR (Gly inserted between Asp324 and Arg325), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2DGR (Gly inserted between Asp324 and Arg325) | — | 4-nitrophenyl beta-D-glucopyranoside | 1.82 | — | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 DGR (Gly inserted between Asp324 and Arg325), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2DGR (Gly inserted between Asp324 and Arg325) | — | 4-nitrophenyl beta-D-glucopyranoside | — | — | 48.9 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 DGR (Gly inserted between Asp324 and Arg325), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2DGR (Gly inserted between Asp324 and Arg325) | — | 4-nitrophenyl beta-D-glucopyranoside | — | 0.95 | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 DGR (Gly inserted between Asp324 and Arg325), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2DGR (Gly inserted between Asp324 and Arg325) | — | 4-nitrophenyl beta-D-glucopyranoside | 17.5 | — | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 DGR (Gly inserted between Asp324 and Arg325), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2DGR (Gly inserted between Asp324 and Arg325) | — | 4-nitrophenyl beta-D-glucopyranoside | — | — | 18.4 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 DGR (Gly inserted between Asp324 and Arg325), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2FGW (D324F/R325W with Gly inserted between them; mimics SsBGL) | — | 4-nitrophenyl beta-D-fucopyranoside | 3.74 | 0.034 | 109 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 FGW (D324F/R325W with Gly inserted between them; mimics SsBGL), 4-nitrophenyl beta-D-fucopyranoside |
| β-glucosidase Td2F2FGW (D324F/R325W with Gly inserted between them; mimics SsBGL) | — | 4-nitrophenyl beta-D-galactopyranoside | 3.11 | 1.39 | 2.23 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 FGW (D324F/R325W with Gly inserted between them; mimics SsBGL), 4-nitrophenyl beta-D-galactopyranoside |
| β-glucosidase Td2F2FGW (D324F/R325W with Gly inserted between them; mimics SsBGL) | — | 4-nitrophenyl beta-D-glucopyranoside | — | 0.089 | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 FGW (D324F/R325W with Gly inserted between them; mimics SsBGL), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2FGW (D324F/R325W with Gly inserted between them; mimics SsBGL) | — | 4-nitrophenyl beta-D-glucopyranoside | 1.16 | — | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 FGW (D324F/R325W with Gly inserted between them; mimics SsBGL), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2FGW (D324F/R325W with Gly inserted between them; mimics SsBGL) | — | 4-nitrophenyl beta-D-glucopyranoside | — | — | 13 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 FGW (D324F/R325W with Gly inserted between them; mimics SsBGL), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2FGW (D324F/R325W with Gly inserted between them; mimics SsBGL) | — | 4-nitrophenyl beta-D-glucopyranoside | — | 1.12 | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 FGW (D324F/R325W with Gly inserted between them; mimics SsBGL), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2FGW (D324F/R325W with Gly inserted between them; mimics SsBGL) | — | 4-nitrophenyl beta-D-glucopyranoside | 17.9 | — | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 FGW (D324F/R325W with Gly inserted between them; mimics SsBGL), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2FGW (D324F/R325W with Gly inserted between them; mimics SsBGL) | — | 4-nitrophenyl beta-D-glucopyranoside | — | — | 16 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 FGW (D324F/R325W with Gly inserted between them; mimics SsBGL), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2MGW (D324M/R325W with Gly inserted between them; mimics TmBGL) | — | 4-nitrophenyl beta-D-fucopyranoside | 19 | 0.13 | 151 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 MGW (D324M/R325W with Gly inserted between them; mimics TmBGL), 4-nitrophenyl beta-D-fucopyranoside |
| β-glucosidase Td2F2MGW (D324M/R325W with Gly inserted between them; mimics TmBGL) | — | 4-nitrophenyl beta-D-galactopyranoside | 9.35 | 0.94 | 9.96 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 MGW (D324M/R325W with Gly inserted between them; mimics TmBGL), 4-nitrophenyl beta-D-galactopyranoside |
| β-glucosidase Td2F2MGW (D324M/R325W with Gly inserted between them; mimics TmBGL) | — | 4-nitrophenyl beta-D-glucopyranoside | — | 0.092 | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 MGW (D324M/R325W with Gly inserted between them; mimics TmBGL), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2MGW (D324M/R325W with Gly inserted between them; mimics TmBGL) | — | 4-nitrophenyl beta-D-glucopyranoside | 5.87 | — | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 MGW (D324M/R325W with Gly inserted between them; mimics TmBGL), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2MGW (D324M/R325W with Gly inserted between them; mimics TmBGL) | — | 4-nitrophenyl beta-D-glucopyranoside | — | — | 63.7 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 MGW (D324M/R325W with Gly inserted between them; mimics TmBGL), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2MGW (D324M/R325W with Gly inserted between them; mimics TmBGL) | — | 4-nitrophenyl beta-D-glucopyranoside | — | 3.5 | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 MGW (D324M/R325W with Gly inserted between them; mimics TmBGL), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2MGW (D324M/R325W with Gly inserted between them; mimics TmBGL) | — | 4-nitrophenyl beta-D-glucopyranoside | 124 | — | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 MGW (D324M/R325W with Gly inserted between them; mimics TmBGL), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2MGW (D324M/R325W with Gly inserted between them; mimics TmBGL) | — | 4-nitrophenyl beta-D-glucopyranoside | — | — | 35.5 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 MGW (D324M/R325W with Gly inserted between them; mimics TmBGL), 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2variant N223Q | — | 4-nitrophenyl beta-D-fucopyranoside | 80.4 | 5 | 16.1 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Q, 4-nitrophenyl beta-D-fucopyranoside |
| β-glucosidase Td2F2variant N223Q | — | 4-nitrophenyl beta-D-galactopyranoside | 32.3 | 17.6 | 1.83 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Q, 4-nitrophenyl beta-D-galactopyranoside |
| β-glucosidase Td2F2variant N223Q | — | 4-nitrophenyl beta-D-glucopyranoside | — | 7.69 | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Q, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2variant N223Q | — | 4-nitrophenyl beta-D-glucopyranoside | 42.5 | — | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Q, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2variant N223Q | — | 4-nitrophenyl beta-D-glucopyranoside | — | — | 5.53 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Q, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2variant N223Q | — | 4-nitrophenyl beta-D-glucopyranoside | — | 34.3 | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Q, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2variant N223Q | — | 4-nitrophenyl beta-D-glucopyranoside | 82.8 | — | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Q, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2variant N223Q | — | 4-nitrophenyl beta-D-glucopyranoside | — | — | 2.41 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Q, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2variant N223Y | — | 4-nitrophenyl beta-D-fucopyranoside | 38.7 | 0.39 | 98.3 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Y, 4-nitrophenyl beta-D-fucopyranoside |
| β-glucosidase Td2F2variant N223Y | — | 4-nitrophenyl beta-D-galactopyranoside | 17.6 | 0.71 | 24.7 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Y, 4-nitrophenyl beta-D-galactopyranoside |
| β-glucosidase Td2F2variant N223Y | — | 4-nitrophenyl beta-D-glucopyranoside | — | 0.85 | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Y, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2variant N223Y | — | 4-nitrophenyl beta-D-glucopyranoside | 36.7 | — | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Y, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2variant N223Y | — | 4-nitrophenyl beta-D-glucopyranoside | — | — | 43.2 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Y, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2variant N223Y | — | 4-nitrophenyl beta-D-glucopyranoside | — | 3.99 | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Y, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2variant N223Y | — | 4-nitrophenyl beta-D-glucopyranoside | 145 | — | — | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Y, 4-nitrophenyl beta-D-glucopyranoside |
| β-glucosidase Td2F2variant N223Y | — | 4-nitrophenyl beta-D-glucopyranoside | — | — | 36.4 | pH 5.5, 75 °C | Open record: β-glucosidase Td2F2 variant N223Y, 4-nitrophenyl beta-D-glucopyranoside |