Values CatLog read from this paper
Directed evolution of mandelate racemase by a novel high-throughput screening method
PMID 27557723doi:10.1007/s00253-016-7790-3Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (48 values) from this paper: mandelate racemase from Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| mandelate racemase | Pseudomonas putida | (R)-o-chloromandelic acid | 0.9 | 14.82 | 0.06074 | pH 7.5, 30 °C | Open record: mandelate racemase, (R)-o-chloromandelic acid |
| mandelate racemase | Pseudomonas putida | (S)-o-chloromandelic acid | 0.99 | 17.74 | 0.05634 | pH 7.5, 30 °C | Open record: mandelate racemase, (S)-o-chloromandelic acid |
| mandelate racemasevariant V22I | Pseudomonas putida | (R)-o-chloromandelic acid | 1.82 | 15.51 | 0.1178 | pH 7.5, 30 °C | Open record: mandelate racemase variant V22I, (R)-o-chloromandelic acid |
| mandelate racemasevariant V22I | Pseudomonas putida | (S)-o-chloromandelic acid | 2.47 | 19.91 | 0.124 | pH 7.5, 30 °C | Open record: mandelate racemase variant V22I, (S)-o-chloromandelic acid |
| mandelate racemasevariant V22I/V29I | Pseudomonas putida | (R)-o-chloromandelic acid | 2.6 | 15.1 | 0.1722 | pH 7.5, 30 °C | Open record: mandelate racemase variant V22I/V29I, (R)-o-chloromandelic acid |
| mandelate racemasevariant V22I/V29I | Pseudomonas putida | (S)-o-chloromandelic acid | 2.35 | 13.74 | 0.1593 | pH 7.5, 30 °C | Open record: mandelate racemase variant V22I/V29I, (S)-o-chloromandelic acid |
| mandelate racemasevariant V22I/V29I/Y54F | Pseudomonas putida | (R)-o-chloromandelic acid | 2.57 | 14.52 | 0.1767 | pH 7.5, 30 °C | Open record: mandelate racemase variant V22I/V29I/Y54F, (R)-o-chloromandelic acid |
| mandelate racemasevariant V22I/V29I/Y54F | Pseudomonas putida | (S)-o-chloromandelic acid | 3.49 | 17.94 | 0.1947 | pH 7.5, 30 °C | Open record: mandelate racemase variant V22I/V29I/Y54F, (S)-o-chloromandelic acid |
| mandelate racemasevariant V22I/Y54F | Pseudomonas putida | (R)-o-chloromandelic acid | 2.4 | 15.97 | 0.1504 | pH 7.5, 30 °C | Open record: mandelate racemase variant V22I/Y54F, (R)-o-chloromandelic acid |
| mandelate racemasevariant V22I/Y54F | Pseudomonas putida | (S)-o-chloromandelic acid | 2.51 | 14.19 | 0.1767 | pH 7.5, 30 °C | Open record: mandelate racemase variant V22I/Y54F, (S)-o-chloromandelic acid |
| mandelate racemasevariant V29I | Pseudomonas putida | (R)-o-chloromandelic acid | 1.45 | 11.99 | 0.1214 | pH 7.5, 30 °C | Open record: mandelate racemase variant V29I, (R)-o-chloromandelic acid |
| mandelate racemasevariant V29I | Pseudomonas putida | (S)-o-chloromandelic acid | 1.83 | 14.35 | 0.1277 | pH 7.5, 30 °C | Open record: mandelate racemase variant V29I, (S)-o-chloromandelic acid |
| mandelate racemasevariant V29I/Y54F | Pseudomonas putida | (R)-o-chloromandelic acid | 1.73 | 9.94 | 0.1741 | pH 7.5, 30 °C | Open record: mandelate racemase variant V29I/Y54F, (R)-o-chloromandelic acid |
| mandelate racemasevariant V29I/Y54F | Pseudomonas putida | (S)-o-chloromandelic acid | 1.96 | 10.62 | 0.1848 | pH 7.5, 30 °C | Open record: mandelate racemase variant V29I/Y54F, (S)-o-chloromandelic acid |
| mandelate racemasevariant Y54F | Pseudomonas putida | (R)-o-chloromandelic acid | 1.23 | 11.66 | 0.1059 | pH 7.5, 30 °C | Open record: mandelate racemase variant Y54F, (R)-o-chloromandelic acid |
| mandelate racemasevariant Y54F | Pseudomonas putida | (S)-o-chloromandelic acid | 1.33 | 10.9 | 0.1222 | pH 7.5, 30 °C | Open record: mandelate racemase variant Y54F, (S)-o-chloromandelic acid |