Values CatLog read from this paper
An alternative allosteric pathway in thermophilic methylglyoxal synthase
PMID 27608544doi:10.1016/j.ijbiomac.2016.09.013Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (22 values) from this paper: methylglyoxal synthase (TMGS) from Thermus sp. GH5.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| methylglyoxal synthase (TMGS) | Thermus sp. GH5 | dihydroxyacetone phosphate0 mM phosphate | 218 | 0.8 | 272 | pH 6.2, 60 °C | Open record: methylglyoxal synthase (TMGS), dihydroxyacetone phosphate |
| methylglyoxal synthase (TMGS) | Thermus sp. GH5 | dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor) | 39 | — | — | pH 6.2, 60 °C | Open record: methylglyoxal synthase (TMGS), dihydroxyacetone phosphate |
| methylglyoxal synthase (TMGS)variant D100N | Thermus sp. GH5 | dihydroxyacetone phosphate0 mM phosphate | 29 | 1 | 30 | pH 6.2, 60 °C | Open record: methylglyoxal synthase (TMGS) variant D100N, dihydroxyacetone phosphate |
| methylglyoxal synthase (TMGS)variant D100N | Thermus sp. GH5 | dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor) | 15 | — | — | pH 6.2, 60 °C | Open record: methylglyoxal synthase (TMGS) variant D100N, dihydroxyacetone phosphate |
| methylglyoxal synthase (TMGS)variant D100V | Thermus sp. GH5 | dihydroxyacetone phosphate0 mM phosphate | 13 | — | — | pH 6.2, 60 °C | Open record: methylglyoxal synthase (TMGS) variant D100V, dihydroxyacetone phosphate |
| methylglyoxal synthase (TMGS)variant D100V | Thermus sp. GH5 | dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor) | 11 | — | — | pH 6.2, 60 °C | Open record: methylglyoxal synthase (TMGS) variant D100V, dihydroxyacetone phosphate |
| methylglyoxal synthase (TMGS)variant R80D/D100R | Thermus sp. GH5 | dihydroxyacetone phosphate0 mM phosphate | 162 | — | — | pH 6.2, 60 °C | Open record: methylglyoxal synthase (TMGS) variant R80D/D100R, dihydroxyacetone phosphate |
| methylglyoxal synthase (TMGS)variant R80D/D100R | Thermus sp. GH5 | dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor) | 30 | — | — | pH 6.2, 60 °C | Open record: methylglyoxal synthase (TMGS) variant R80D/D100R, dihydroxyacetone phosphate |
| methylglyoxal synthase (TMGS)variant R80I | Thermus sp. GH5 | dihydroxyacetone phosphate0 mM phosphate | 15 | 1.2 | 12.5 | pH 6.2, 60 °C | Open record: methylglyoxal synthase (TMGS) variant R80I, dihydroxyacetone phosphate |
| methylglyoxal synthase (TMGS)variant R80I | Thermus sp. GH5 | dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor) | 4 | — | — | pH 6.2, 60 °C | Open record: methylglyoxal synthase (TMGS) variant R80I, dihydroxyacetone phosphate |
| methylglyoxal synthase (TMGS)variant R80K | Thermus sp. GH5 | dihydroxyacetone phosphate0 mM phosphate | 38 | 0.9 | 42 | pH 6.2, 60 °C | Open record: methylglyoxal synthase (TMGS) variant R80K, dihydroxyacetone phosphate |
| methylglyoxal synthase (TMGS)variant R80K | Thermus sp. GH5 | dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor) | 23 | — | — | pH 6.2, 60 °C | Open record: methylglyoxal synthase (TMGS) variant R80K, dihydroxyacetone phosphate |
| methylglyoxal synthase (TMGS)variant R80Q | Thermus sp. GH5 | dihydroxyacetone phosphate0 mM phosphate | 8 | — | — | pH 6.2, 60 °C | Open record: methylglyoxal synthase (TMGS) variant R80Q, dihydroxyacetone phosphate |
| methylglyoxal synthase (TMGS)variant R80Q | Thermus sp. GH5 | dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor) | 1 | — | — | pH 6.2, 60 °C | Open record: methylglyoxal synthase (TMGS) variant R80Q, dihydroxyacetone phosphate |