Values CatLog read from this paper
Improving the catalytic efficiency of Bacillus pumilus CotA-laccase by site-directed mutagenesis
PMID 27826721doi:10.1007/s00253-016-7962-1Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (36 values) from this paper: CotA-laccase from Bacillus pumilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CotA-laccase | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 38.61 | 0.252 | 153.2 | pH 3.5, 50 °C | Open record: CotA-laccase, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant G417L | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 51.7 | 0.127 | 406.8 | pH 3.5, 50 °C | Open record: CotA-laccase variant G417L, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant L386W | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 46.38 | 0.203 | 228.5 | pH 3.5, 50 °C | Open record: CotA-laccase variant L386W, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant L386W/G417L | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 65.07 | 0.098 | 664.0 | pH 3.5, 50 °C | Open record: CotA-laccase variant L386W/G417L, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant L386W/G417L/D152F | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 30.11 | 0.287 | 104.9 | pH 3.5, 50 °C | Open record: CotA-laccase variant L386W/G417L/D152F, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant L386W/G417L/D152W | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 45.89 | 0.212 | 216.5 | pH 3.5, 50 °C | Open record: CotA-laccase variant L386W/G417L/D152W, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant L386W/G417L/D152Y | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 83.56 | 0.223 | 374.7 | pH 3.5, 50 °C | Open record: CotA-laccase variant L386W/G417L/D152Y, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant L386W/G417L/D195F | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 40.78 | 0.246 | 165.8 | pH 3.5, 50 °C | Open record: CotA-laccase variant L386W/G417L/D195F, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant L386W/G417L/D195W | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 68.89 | 0.286 | 240.9 | pH 3.5, 50 °C | Open record: CotA-laccase variant L386W/G417L/D195W, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant L386W/G417L/G57F | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 83.35 | 0.115 | 724.8 | pH 3.5, 50 °C | Open record: CotA-laccase variant L386W/G417L/G57F, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant L386W/G417L/G57L | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 58.65 | 0.137 | 428.1 | pH 3.5, 50 °C | Open record: CotA-laccase variant L386W/G417L/G57L, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
| CotA-laccasevariant L386W/G417L/G57W | Bacillus pumilus | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 40.66 | 0.278 | 146.3 | pH 3.5, 50 °C | Open record: CotA-laccase variant L386W/G417L/G57W, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |