Values CatLog read from this paper

Enzymatic Activity and Thermodynamic Stability of Biliverdin IXβ Reductase Are Maintained by an Active Site Serine

PMID 27897348doi:10.1002/chem.201604517Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 27 records (37 values) from this paper: biliverdin IXβ reductase (BLVRB) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.5.1.30

Records read from this paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsOpen record
biliverdin IXβ reductase (BLVRB)Homo sapiensflavin mononucleotide—0.102—25 °COpen record: biliverdin IXβ reductase (BLVRB), flavin mononucleotide
biliverdin IXβ reductase (BLVRB)Homo sapiensflavin mononucleotide—0.3—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB), flavin mononucleotide
biliverdin IXβ reductase (BLVRB)Homo sapiensNADH—0.118—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB), NADH
biliverdin IXβ reductase (BLVRB)Homo sapiensNADPH0.1710.00832125 °COpen record: biliverdin IXβ reductase (BLVRB), NADPH
biliverdin IXβ reductase (BLVRB)Homo sapiensNADPHNADPH or NADH varied at a fixed FMN concentration of 150 µM—0.0024—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB), NADPH
biliverdin IXβ reductase (BLVRB)Homo sapiensNADPHFMN (5-200 µM) and NADPH (5-100 µM) varied; cosubstrate FMN0.2370.002499 calc.pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB), NADPH
biliverdin IXβ reductase (BLVRB)variant H132AHomo sapiensflavin mononucleotide—0.222—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant H132A, flavin mononucleotide
biliverdin IXβ reductase (BLVRB)variant H132AHomo sapiensNADPH0.2370.004948 calc.pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant H132A, NADPH
biliverdin IXβ reductase (BLVRB)variant R124AHomo sapiensflavin mononucleotide—0.095—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant R124A, flavin mononucleotide
biliverdin IXβ reductase (BLVRB)variant R124AHomo sapiensNADPH0.0970.004522 calc.pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant R124A, NADPH
biliverdin IXβ reductase (BLVRB)variant R174AHomo sapiensNADPH0.40.003130 calc.pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant R174A, NADPH
biliverdin IXβ reductase (BLVRB)variant R35AHomo sapiensNADH—0.108—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant R35A, NADH
biliverdin IXβ reductase (BLVRB)variant R35AHomo sapiensNADPH—0.059—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant R35A, NADPH
biliverdin IXβ reductase (BLVRB)variant R35SHomo sapiensNADH—0.107—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant R35S, NADH
biliverdin IXβ reductase (BLVRB)variant R35SHomo sapiensNADPH—0.029—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant R35S, NADPH
biliverdin IXβ reductase (BLVRB)variant R39AHomo sapiensNADH—0.305—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant R39A, NADH
biliverdin IXβ reductase (BLVRB)variant R39AHomo sapiensNADPH—0.0016—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant R39A, NADPH
biliverdin IXβ reductase (BLVRB)variant R78AHomo sapiensNADH—0.15—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant R78A, NADH
biliverdin IXβ reductase (BLVRB)variant R78AHomo sapiensNADPH—0.0047—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant R78A, NADPH
biliverdin IXβ reductase (BLVRB)variant S111AHomo sapiensflavin mononucleotide—0.137—25 °COpen record: biliverdin IXβ reductase (BLVRB) variant S111A, flavin mononucleotide
biliverdin IXβ reductase (BLVRB)variant S111AHomo sapiensNADPH0.0250.00673.725 °COpen record: biliverdin IXβ reductase (BLVRB) variant S111A, NADPH
biliverdin IXβ reductase (BLVRB)variant S111LHomo sapiensflavin mononucleotide—0.162—25 °COpen record: biliverdin IXβ reductase (BLVRB) variant S111L, flavin mononucleotide
biliverdin IXβ reductase (BLVRB)variant S111LHomo sapiensNADPH0.010.00581.725 °COpen record: biliverdin IXβ reductase (BLVRB) variant S111L, NADPH
biliverdin IXβ reductase (BLVRB)variant W116AHomo sapiensNADH—0.186—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant W116A, NADH
biliverdin IXβ reductase (BLVRB)variant W116AHomo sapiensNADPH—0.0017—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant W116A, NADPH
biliverdin IXβ reductase (BLVRB)variant W116FHomo sapiensNADH—0.319—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant W116F, NADH
biliverdin IXβ reductase (BLVRB)variant W116FHomo sapiensNADPH—0.0011—pH 7.2, 30 °COpen record: biliverdin IXβ reductase (BLVRB) variant W116F, NADPH