Values CatLog read from this paper

The T296V Mutant of Amorpha-4,11-diene Synthase Is Defective in Allylic Diphosphate Isomerization but Retains the Ability To Cyclize the Intermediate (3R)-Nerolidyl Diphosphate to Amorpha-4,11-diene

PMID 27933789doi:10.1021/acs.biochem.6b01004Open in Browse

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CatLog read 5 records (15 values) from this paper: amorpha-4,11-diene synthase from Artemisia annua.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.2.3.24

Records read from this paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsOpen record
amorpha-4,11-diene synthaseArtemisia annua(2E,6E)-farnesyl diphosphate0.01630.01531.06Open record: amorpha-4,11-diene synthase, (2E,6E)-farnesyl diphosphate
amorpha-4,11-diene synthasevariant T296AArtemisia annua(2E,6E)-farnesyl diphosphate0.00590.01080.55Open record: amorpha-4,11-diene synthase variant T296A, (2E,6E)-farnesyl diphosphate
amorpha-4,11-diene synthasevariant T296IArtemisia annua(2E,6E)-farnesyl diphosphate0.00130.01980.07Open record: amorpha-4,11-diene synthase variant T296I, (2E,6E)-farnesyl diphosphate
amorpha-4,11-diene synthasevariant T296SArtemisia annua(2E,6E)-farnesyl diphosphate0.00750.0330.23Open record: amorpha-4,11-diene synthase variant T296S, (2E,6E)-farnesyl diphosphate
amorpha-4,11-diene synthasevariant T296VArtemisia annua(2E,6E)-farnesyl diphosphate0.00150.02820.05Open record: amorpha-4,11-diene synthase variant T296V, (2E,6E)-farnesyl diphosphate