Values CatLog read from this paper
Aminoalcohol-Induced Activation of Organophosphorus Hydrolase (OPH) towards Diisopropylfluorophosphate (DFP)
PMID 28085964doi:10.1371/journal.pone.0169937Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 60 records (154 values) from this paper: organophosphorus hydrolase (OPH) from Sphingobium fuliginis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphateno additive (control) | 117 | 3.01 | 41.17 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate100 mM triethanolamine (TEA) | 231 | 4 | 59.87 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate200 mM triethanolamine (TEA) | 314 | 3.81 | 85.59 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM triethanolamine (TEA) | 419 | 4.06 | 105.6 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate400 mM triethanolamine (TEA) | 477 | 4.32 | 113.0 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate100 mM diethanolamine (DEA) | 153 | 3.05 | 50.4 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate200 mM diethanolamine (DEA) | 237 | 3.42 | 70.6 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM diethanolamine (DEA) | 277 | 3.16 | 87.41 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate400 mM diethanolamine (DEA) | 312 | 3.35 | 93.22 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate100 mM monoethanolamine (MEA) | 143 | 3.18 | 45.31 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate200 mM monoethanolamine (MEA) | 159 | 3.19 | 50.21 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM monoethanolamine (MEA) | 192 | 2.8 | 69.26 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate400 mM monoethanolamine (MEA) | 209 | 3.15 | 66.46 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM ethanol | 123 | 3.08 | 40.25 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM glycerol | 115 | 3.38 | 34.12 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM methanol | 123 | 3.18 | 38.75 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate100 mM ethylamine | 126 | 4.01 | 32.01 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate200 mM ethylamine | 152 | 4.21 | 36.24 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM ethylamine | 180 | 4.56 | 39.73 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate100 mM diethylamine | 121 | 3.97 | 31.6 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate200 mM diethylamine | 145 | 4.53 | 32.56 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM diethylamine | 163 | 4.67 | 35.48 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate100 mM triethylamine | 114 | 3.9 | 30.28 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate200 mM triethylamine | 126 | 4.04 | 32.56 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM triethylamine | 148 | 4.52 | 33.93 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate100 mM methylamine | 149 | 3.95 | 37.91 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate200 mM methylamine | 179 | 4.26 | 42.19 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM methylamine | 193 | 4.44 | 43.43 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM ethanol plus 300 mM ethylamine | 195 | 4.32 | 45.1 calc. | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM ethanol plus 300 mM diethylamine | 178 | 4.41 | 40.4 calc. | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM ethanol plus 300 mM triethylamine | 155 | 4.45 | 34.8 calc. | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM ethanol plus 300 mM methylamine | 216 | 4.17 | 51.8 calc. | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM 3-amino-1-propanol | 142 | 4.61 | 30.8 calc. | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM 1-amino-2-propanol | 195 | 2.92 | 66.8 calc. | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM 2-amino-2-methyl-1-propanol | 211 | 2.95 | 71.5 calc. | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM triisopropanolamine | 361 | 4.21 | 85.7 calc. | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM thiodiglycol | 295 | 3.97 | 74.3 calc. | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM thiodipropanol | 151 | 4.77 | 31.7 calc. | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphateno TEA (0 mM); 0 mM NaF | — | 2.95 | 51.19 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphateno TEA (0 mM); 1 mM NaF | — | 2.99 | 18.49 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphateno TEA (0 mM); 2 mM NaF | — | 3.57 | 11.93 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphateno TEA (0 mM); 3 mM NaF | — | 4.45 | 6.45 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate100 mM triethanolamine (TEA); 0 mM NaF | — | 3.52 | 69.9 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate100 mM triethanolamine (TEA); 1 mM NaF | — | 4.23 | 27.79 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate100 mM triethanolamine (TEA); 2 mM NaF | — | 4.87 | 18.62 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate100 mM triethanolamine (TEA); 3 mM NaF | — | 5.58 | 11.73 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate200 mM triethanolamine (TEA); 0 mM NaF | — | 3.26 | 103.3 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate200 mM triethanolamine (TEA); 1 mM NaF | — | 3.87 | 50.32 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate200 mM triethanolamine (TEA); 2 mM NaF | — | 4.54 | 32.55 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate200 mM triethanolamine (TEA); 3 mM NaF | — | 5.04 | 21.17 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM triethanolamine (TEA); 0 mM NaF | — | 3.49 | 126.1 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM triethanolamine (TEA); 1 mM NaF | — | 4.06 | 74.78 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM triethanolamine (TEA); 2 mM NaF | — | 4.58 | 47.84 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH) | Sphingobium fuliginis | diisopropylfluorophosphate300 mM triethanolamine (TEA); 3 mM NaF | — | 4.89 | 36.23 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH), diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH)variant F132Y | Sphingobium fuliginis | diisopropylfluorophosphateno additive (0 mM TEA) | 490 | 3.2 | 153.4 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH) variant F132Y, diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH)variant F132Y | Sphingobium fuliginis | diisopropylfluorophosphate300 mM triethanolamine (TEA) | 1.82 × 10³ | 4.2 | 432.5 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH) variant F132Y, diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH)variant F132Y/L140Y | Sphingobium fuliginis | diisopropylfluorophosphateno additive (0 mM TEA) | 580 | 3.1 | 185.8 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH) variant F132Y/L140Y, diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH)variant F132Y/L140Y | Sphingobium fuliginis | diisopropylfluorophosphate300 mM triethanolamine (TEA) | 2.17 × 10³ | 4 | 541.7 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH) variant F132Y/L140Y, diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH)variant L140Y | Sphingobium fuliginis | diisopropylfluorophosphateno additive (0 mM TEA) | 310 | 3.2 | 97.3 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH) variant L140Y, diisopropylfluorophosphate |
| organophosphorus hydrolase (OPH)variant L140Y | Sphingobium fuliginis | diisopropylfluorophosphate300 mM triethanolamine (TEA) | 1.42 × 10³ | 4.5 | 319.6 | pH 8, 25 °C | Open record: organophosphorus hydrolase (OPH) variant L140Y, diisopropylfluorophosphate |