CatLogenzyme kinetics catalog

Values CatLog read from this paper

Engineering the cofactor specificity of an alcohol dehydrogenase via single mutations or insertions distal to the 2'-phosphate group of NADP(H)

PMID 28201792doi:10.1093/protein/gzx009Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 28 records (70 values) from this paper: alcohol dehydrogenase D (AdhD) from Pyrococcus furiosus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
alcohol dehydrogenase D (AdhD)Pyrococcus furiosus2,3-butanediolcosubstrate NAD+2.6120.21pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)Pyrococcus furiosus2,3-butanediolcosubstrate NADP+0.0730.440.16pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)Pyrococcus furiosusNAD+—0.2311pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)Pyrococcus furiosusNADP+—0.00749.8pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211CPyrococcus furiosus2,3-butanediolcosubstrate NAD+1.12400.0046pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211CPyrococcus furiosus2,3-butanediolcosubstrate NADP+0.929.60.096pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211CPyrococcus furiosusNAD+—0.482.3pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211CPyrococcus furiosusNADP+—0.02537pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsAPyrococcus furiosus2,3-butanediolcosubstrate NAD+1.72600.0065pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsAPyrococcus furiosus2,3-butanediolcosubstrate NADP+2.1900.023pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsAPyrococcus furiosusNAD+—0.1214pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsAPyrococcus furiosusNADP+—0.365.7pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsCPyrococcus furiosus2,3-butanediolcosubstrate NAD+0.43760.0057pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsCPyrococcus furiosus2,3-butanediolcosubstrate NADP+0.865.50.16pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsCPyrococcus furiosusNAD+—0.0974.4pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsCPyrococcus furiosusNADP+—0.127.4pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsGPyrococcus furiosus2,3-butanediolcosubstrate NAD+6.51100.059pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsGPyrococcus furiosus2,3-butanediolcosubstrate NADP+0.521.50.34pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsGPyrococcus furiosusNAD+—0.2922pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsGPyrococcus furiosusNADP+—0.03316pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsSPyrococcus furiosus2,3-butanediolcosubstrate NAD+6.71.1 × 10³0.0061pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsSPyrococcus furiosus2,3-butanediolcosubstrate NADP+2.1170.12pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsSPyrococcus furiosusNAD+—1.64.2pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211InsSPyrococcus furiosusNADP+—0.06632pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211SPyrococcus furiosus2,3-butanediolcosubstrate NAD+41000.04pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211SPyrococcus furiosus2,3-butanediolcosubstrate NADP+0.290.910.31pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211SPyrococcus furiosusNAD+—0.140pH 8.8, 45 °COpen
alcohol dehydrogenase D (AdhD)variant G211SPyrococcus furiosusNADP+—0.01519pH 8.8, 45 °COpen