Values CatLog read from this paper
Isolation and characterization of a thermostable F420:NADPH oxidoreductase from Thermobifida fusca
PMID 28411200doi:10.1074/jbc.m117.787754Open in Browse
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CatLog read 28 records (76 values) from this paper: F420:NADPH oxidoreductase (Tfu-FNO) from Thermobifida fusca.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| F420:NADPH oxidoreductase (Tfu-FNO) | Thermobifida fusca | coenzyme F420 | — | 0.002 | — | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO), coenzyme F420 |
| F420:NADPH oxidoreductase (Tfu-FNO) | Thermobifida fusca | NADH | 2.2 | 14 | 0.16 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO), NADH |
| F420:NADPH oxidoreductase (Tfu-FNO) | Thermobifida fusca | NADPH | 3.3 | 0.0073 | 450 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO), NADPH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant R51A | Thermobifida fusca | NADH | 3.2 | 8.6 | 0.37 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant R51A, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant R51A | Thermobifida fusca | NADPH | — | — | 6.2 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant R51A, NADPH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant R51E/R55A | Thermobifida fusca | NADH | 1.6 | 10 | 0.16 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant R51E/R55A, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant R51E/R55N | Thermobifida fusca | NADH | 2.7 | 6.5 | 0.42 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant R51E/R55N, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant R51E/R55S | Thermobifida fusca | NADH | 4.9 | 32 | 0.15 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant R51E/R55S, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant R51V | Thermobifida fusca | NADH | 3.4 | 8.7 | 0.29 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant R51V, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant R51V | Thermobifida fusca | NADPH | — | — | 9.3 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant R51V, NADPH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant R51V/R55V | Thermobifida fusca | NADH | 2.8 | 10 | 0.28 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant R51V/R55V, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant R55A | Thermobifida fusca | NADH | 3 | 7 | 0.42 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant R55A, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant R55A | Thermobifida fusca | NADPH | 8.8 | 0.029 | 300 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant R55A, NADPH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant R55N | Thermobifida fusca | NADH | 2.8 | 6.3 | 0.44 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant R55N, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant R55S | Thermobifida fusca | NADH | 3.5 | 4.4 | 0.79 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant R55S, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant R55S | Thermobifida fusca | NADPH | 6.9 | 0.17 | 41 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant R55S, NADPH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant R55V | Thermobifida fusca | NADH | 3.2 | 9.6 | 0.33 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant R55V, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant R55V | Thermobifida fusca | NADPH | — | 0.049 | — | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant R55V, NADPH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant S50E | Thermobifida fusca | NADH | 2.7 | 3.2 | 0.84 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant S50E, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant S50E/R55A | Thermobifida fusca | NADH | 2.3 | 20 | 0.12 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant S50E/R55A, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant S50E/R55V | Thermobifida fusca | NADH | 1.8 | 9.8 | 0.18 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant S50E/R55V, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant S50Q | Thermobifida fusca | NADH | 4.2 | 8.2 | 0.51 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant S50Q, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant T28A | Thermobifida fusca | NADH | 2.6 | 5 | 0.52 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant T28A, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant T28A | Thermobifida fusca | NADPH | 14 | 0.019 | 720 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant T28A, NADPH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant T28A/R51V | Thermobifida fusca | NADH | 2.7 | 12 | 0.23 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant T28A/R51V, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant T28A/R51V/R55V | Thermobifida fusca | NADH | 3.3 | 12 | 0.28 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant T28A/R51V/R55V, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant T28A/R55A | Thermobifida fusca | NADH | 2.5 | 5.4 | 0.46 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant T28A/R55A, NADH |
| F420:NADPH oxidoreductase (Tfu-FNO)variant T28A/R55A | Thermobifida fusca | NADPH | 3.3 | 0.093 | 3.5 | pH 6, 25 °C | Open record: F420:NADPH oxidoreductase (Tfu-FNO) variant T28A/R55A, NADPH |