Values CatLog read from this paper
Activity modulation of the oligopeptidase B from Serratia proteamaculans by site-directed mutagenesis of amino acid residues surrounding catalytic triad histidine
PMID 28554571doi:10.1016/j.biochi.2017.05.013Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 30 records (66 values) from this paper: oligopeptidase B (PSP) from Serratia proteamaculans.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| oligopeptidase B (PSP) | Serratia proteamaculans | benzyloxycarbonyl-Arg-Arg-p-nitroanilide | 33.98 | 0.00473 | 7.18 × 10³ | pH 8, 25 °C | Open record: oligopeptidase B (PSP), benzyloxycarbonyl-Arg-Arg-p-nitroanilide |
| oligopeptidase B (PSP) | Serratia proteamaculans | benzyloxycarbonyl-Lys-Arg-p-nitroanilide | 52.34 | 0.0194 | 2.7 × 10³ | pH 8, 25 °C | Open record: oligopeptidase B (PSP), benzyloxycarbonyl-Lys-Arg-p-nitroanilide |
| oligopeptidase B (PSP) | Serratia proteamaculans | Nα-benzoyl-D,L-arginine p-nitroanilide | 14.35 | 0.0836 | 172 | pH 8, 25 °C | Open record: oligopeptidase B (PSP), Nα-benzoyl-D,L-arginine p-nitroanilide |
| oligopeptidase B (PSP)variant D647A | Serratia proteamaculans | benzyloxycarbonyl-Arg-Arg-p-nitroanilide | 30.84 | 0.00864 | 3.57 × 10³ | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant D647A, benzyloxycarbonyl-Arg-Arg-p-nitroanilide |
| oligopeptidase B (PSP)variant D647A | Serratia proteamaculans | benzyloxycarbonyl-Lys-Arg-p-nitroanilide | 25.23 | 0.00552 | 4.57 × 10³ | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant D647A, benzyloxycarbonyl-Lys-Arg-p-nitroanilide |
| oligopeptidase B (PSP)variant D647A | Serratia proteamaculans | Nα-benzoyl-D,L-arginine p-nitroanilide | 6.5 | 0.0316 | 205 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant D647A, Nα-benzoyl-D,L-arginine p-nitroanilide |
| oligopeptidase B (PSP)variant D649A | Serratia proteamaculans | benzyloxycarbonyl-Arg-Arg-p-nitroanilide | 32.36 | 0.0106 | 3.07 × 10³ | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant D649A, benzyloxycarbonyl-Arg-Arg-p-nitroanilide |
| oligopeptidase B (PSP)variant D649A | Serratia proteamaculans | benzyloxycarbonyl-Lys-Arg-p-nitroanilide | 51.22 | 0.00861 | 5.95 × 10³ | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant D649A, benzyloxycarbonyl-Lys-Arg-p-nitroanilide |
| oligopeptidase B (PSP)variant D649A | Serratia proteamaculans | Nα-benzoyl-D,L-arginine p-nitroanilide | 16.8 | 0.0279 | 602 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant D649A, Nα-benzoyl-D,L-arginine p-nitroanilide |
| oligopeptidase B (PSP)variant E125A | Serratia proteamaculans | benzyloxycarbonyl-Arg-Arg-p-nitroanilide | 45.37 | 0.0028 | 1.62 × 10⁴ | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant E125A, benzyloxycarbonyl-Arg-Arg-p-nitroanilide |
| oligopeptidase B (PSP)variant E125A | Serratia proteamaculans | benzyloxycarbonyl-Lys-Arg-p-nitroanilide | 85.55 | 0.0172 | 4.98 × 10³ | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant E125A, benzyloxycarbonyl-Lys-Arg-p-nitroanilide |
| oligopeptidase B (PSP)variant E125A | Serratia proteamaculans | Nα-benzoyl-D,L-arginine p-nitroanilide | 63.71 | 0.046 | 1395 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant E125A, Nα-benzoyl-D,L-arginine p-nitroanilide |
| oligopeptidase B (PSP)variant E75A | Serratia proteamaculans | benzyloxycarbonyl-Arg-Arg-p-nitroanilide | — | — | 370 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant E75A, benzyloxycarbonyl-Arg-Arg-p-nitroanilide |
| oligopeptidase B (PSP)variant E75A | Serratia proteamaculans | benzyloxycarbonyl-Lys-Arg-p-nitroanilide | — | — | 155 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant E75A, benzyloxycarbonyl-Lys-Arg-p-nitroanilide |
| oligopeptidase B (PSP)variant E75A | Serratia proteamaculans | Nα-benzoyl-D,L-arginine p-nitroanilide | — | — | 35 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant E75A, Nα-benzoyl-D,L-arginine p-nitroanilide |
| oligopeptidase B (PSP)variant E75A/R658A | Serratia proteamaculans | Nα-benzoyl-D,L-arginine p-nitroanilide | — | — | 0.17 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant E75A/R658A, Nα-benzoyl-D,L-arginine p-nitroanilide |
| oligopeptidase B (PSP)variant E96A | Serratia proteamaculans | Nα-benzoyl-D,L-arginine p-nitroanilide | — | — | 2.7 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant E96A, Nα-benzoyl-D,L-arginine p-nitroanilide |
| oligopeptidase B (PSP)variant E96A/E125A | Serratia proteamaculans | Nα-benzoyl-D,L-arginine p-nitroanilide | — | — | 1.3 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant E96A/E125A, Nα-benzoyl-D,L-arginine p-nitroanilide |
| oligopeptidase B (PSP)variant E96A/K660A | Serratia proteamaculans | benzyloxycarbonyl-Arg-Arg-p-nitroanilide | — | — | 250 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant E96A/K660A, benzyloxycarbonyl-Arg-Arg-p-nitroanilide |
| oligopeptidase B (PSP)variant E96A/K660A | Serratia proteamaculans | benzyloxycarbonyl-Lys-Arg-p-nitroanilide | — | — | 133 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant E96A/K660A, benzyloxycarbonyl-Lys-Arg-p-nitroanilide |
| oligopeptidase B (PSP)variant E96A/K660A | Serratia proteamaculans | Nα-benzoyl-D,L-arginine p-nitroanilide | — | — | 17 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant E96A/K660A, Nα-benzoyl-D,L-arginine p-nitroanilide |
| oligopeptidase B (PSP)variant K655A | Serratia proteamaculans | benzyloxycarbonyl-Arg-Arg-p-nitroanilide | 11.31 | 0.0317 | 357 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant K655A, benzyloxycarbonyl-Arg-Arg-p-nitroanilide |
| oligopeptidase B (PSP)variant K655A | Serratia proteamaculans | benzyloxycarbonyl-Lys-Arg-p-nitroanilide | 27.35 | 0.143 | 192 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant K655A, benzyloxycarbonyl-Lys-Arg-p-nitroanilide |
| oligopeptidase B (PSP)variant K655A | Serratia proteamaculans | Nα-benzoyl-D,L-arginine p-nitroanilide | 4.727 | 0.51 | 9.27 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant K655A, Nα-benzoyl-D,L-arginine p-nitroanilide |
| oligopeptidase B (PSP)variant K660A | Serratia proteamaculans | benzyloxycarbonyl-Arg-Arg-p-nitroanilide | 24.05 | 0.00954 | 2.52 × 10³ | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant K660A, benzyloxycarbonyl-Arg-Arg-p-nitroanilide |
| oligopeptidase B (PSP)variant K660A | Serratia proteamaculans | benzyloxycarbonyl-Lys-Arg-p-nitroanilide | 29.97 | 0.01086 | 2.77 × 10³ | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant K660A, benzyloxycarbonyl-Lys-Arg-p-nitroanilide |
| oligopeptidase B (PSP)variant K660A | Serratia proteamaculans | Nα-benzoyl-D,L-arginine p-nitroanilide | 6.273 | 0.0708 | 88.7 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant K660A, Nα-benzoyl-D,L-arginine p-nitroanilide |
| oligopeptidase B (PSP)variant PSP 1-655 | Serratia proteamaculans | Nα-benzoyl-D,L-arginine p-nitroanilide | — | — | 0.025 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant PSP 1-655, Nα-benzoyl-D,L-arginine p-nitroanilide |
| oligopeptidase B (PSP)variant R658A | Serratia proteamaculans | Nα-benzoyl-D,L-arginine p-nitroanilide | — | — | 0.23 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant R658A, Nα-benzoyl-D,L-arginine p-nitroanilide |
| oligopeptidase B (PSP)variant R658A/K660A | Serratia proteamaculans | Nα-benzoyl-D,L-arginine p-nitroanilide | — | — | 0.18 | pH 8, 25 °C | Open record: oligopeptidase B (PSP) variant R658A/K660A, Nα-benzoyl-D,L-arginine p-nitroanilide |