Values CatLog read from this paper
Investigation of pyrophosphate versus ATP substrate selection in the Entamoeba histolytica acetate kinase
PMID 28724909doi:10.1038/s41598-017-06156-5Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 22 records (66 values) from this paper: EhACK (acetate kinase, PPi-dependent), MtACK (acetate kinase) from Entamoeba histolytica, Methanosarcina thermophila.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| EhACK (acetate kinase, PPi-dependent) | Entamoeba histolytica | acetate | 1.4 | 166 | 0.0086 | pH 5.5, 45 °C | Open record: EhACK (acetate kinase, PPi-dependent), acetate |
| EhACK (acetate kinase, PPi-dependent) | Entamoeba histolytica | acetyl phosphate | 266 | 0.57 | 467 | pH 7, 37 °C | Open record: EhACK (acetate kinase, PPi-dependent), acetyl phosphate |
| EhACK (acetate kinase, PPi-dependent) | Entamoeba histolytica | diphosphate | 0.9 | 2.1 | 0.44 | pH 5.5, 45 °C | Open record: EhACK (acetate kinase, PPi-dependent), diphosphate |
| EhACK (acetate kinase, PPi-dependent) | Entamoeba histolytica | phosphate | 196 | 14 | 14 | pH 7, 37 °C | Open record: EhACK (acetate kinase, PPi-dependent), phosphate |
| EhACK (acetate kinase, PPi-dependent)variant D272A/R274A/Q323G/M324I | Entamoeba histolytica | acetyl phosphate | 0.052 | 0.66 | 0.08 | pH 7, 37 °C | Open record: EhACK (acetate kinase, PPi-dependent) variant D272A/R274A/Q323G/M324I, acetyl phosphate |
| EhACK (acetate kinase, PPi-dependent)variant D272A/R274A/Q323G/M324I | Entamoeba histolytica | phosphate | 0.076 | 18 | 0.004 | pH 7, 37 °C | Open record: EhACK (acetate kinase, PPi-dependent) variant D272A/R274A/Q323G/M324I, phosphate |
| EhACK (acetate kinase, PPi-dependent)variant Q323A/M324A | Entamoeba histolytica | acetate | 0.51 | 566 | 9 × 10⁻⁴ | pH 5.5, 45 °C | Open record: EhACK (acetate kinase, PPi-dependent) variant Q323A/M324A, acetate |
| EhACK (acetate kinase, PPi-dependent)variant Q323A/M324A | Entamoeba histolytica | acetyl phosphate | 14 | 0.34 | 42 | pH 7, 37 °C | Open record: EhACK (acetate kinase, PPi-dependent) variant Q323A/M324A, acetyl phosphate |
| EhACK (acetate kinase, PPi-dependent)variant Q323A/M324A | Entamoeba histolytica | diphosphate | 0.36 | 2.3 | 0.16 | pH 5.5, 45 °C | Open record: EhACK (acetate kinase, PPi-dependent) variant Q323A/M324A, diphosphate |
| EhACK (acetate kinase, PPi-dependent)variant Q323A/M324A | Entamoeba histolytica | phosphate | 20 | 8.2 | 2.5 | pH 7, 37 °C | Open record: EhACK (acetate kinase, PPi-dependent) variant Q323A/M324A, phosphate |
| EhACK (acetate kinase, PPi-dependent)variant Q323G/M324I | Entamoeba histolytica | acetate | 0.51 | 372 | 0.0014 | pH 5.5, 45 °C | Open record: EhACK (acetate kinase, PPi-dependent) variant Q323G/M324I, acetate |
| EhACK (acetate kinase, PPi-dependent)variant Q323G/M324I | Entamoeba histolytica | acetyl phosphate | 32 | 0.47 | 68 | pH 7, 37 °C | Open record: EhACK (acetate kinase, PPi-dependent) variant Q323G/M324I, acetyl phosphate |
| EhACK (acetate kinase, PPi-dependent)variant Q323G/M324I | Entamoeba histolytica | diphosphate | 0.45 | 2 | 0.23 | pH 5.5, 45 °C | Open record: EhACK (acetate kinase, PPi-dependent) variant Q323G/M324I, diphosphate |
| EhACK (acetate kinase, PPi-dependent)variant Q323G/M324I | Entamoeba histolytica | phosphate | 28 | 7.3 | 3.8 | pH 7, 37 °C | Open record: EhACK (acetate kinase, PPi-dependent) variant Q323G/M324I, phosphate |
| MtACK (acetate kinase) | Methanosarcina thermophila | acetate | 789 | 20 | 39 | pH 7.5, 37 °C | Open record: MtACK (acetate kinase), acetate |
| MtACK (acetate kinase) | Methanosarcina thermophila | acetyl phosphate | 1.55 × 10³ | 1.2 | 1294 | pH 7.5, 37 °C | Open record: MtACK (acetate kinase), acetyl phosphate |
| MtACK (acetate kinase) | Methanosarcina thermophila | ADP | 1915 | 1.8 | 1073 | pH 7.5, 37 °C | Open record: MtACK (acetate kinase), ADP |
| MtACK (acetate kinase) | Methanosarcina thermophila | ATP | 711 | 1.7 | 421 | pH 7.5, 37 °C | Open record: MtACK (acetate kinase), ATP |
| MtACK (acetate kinase)variant G331Q/I332M | Methanosarcina thermophila | acetate | 17 | 302 | 0.057 | pH 7.5, 37 °C | Open record: MtACK (acetate kinase) variant G331Q/I332M, acetate |
| MtACK (acetate kinase)variant G331Q/I332M | Methanosarcina thermophila | acetyl phosphate | 12 | 1.7 | 7.2 | pH 7.5, 37 °C | Open record: MtACK (acetate kinase) variant G331Q/I332M, acetyl phosphate |
| MtACK (acetate kinase)variant G331Q/I332M | Methanosarcina thermophila | ADP | 12 | 5.7 | 2 | pH 7.5, 37 °C | Open record: MtACK (acetate kinase) variant G331Q/I332M, ADP |
| MtACK (acetate kinase)variant G331Q/I332M | Methanosarcina thermophila | ATP | 14 | 9.4 | 1.5 | pH 7.5, 37 °C | Open record: MtACK (acetate kinase) variant G331Q/I332M, ATP |