Values CatLog read from this paper
Understanding the role of residues around the active site tunnel towards generating a glucose-tolerant β-glucosidase from Agrobacterium tumefaciens 5A
PMID 28873987doi:10.1093/protein/gzx039Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 38 records (114 values) from this paper: beta-glucosidase H0HC94 from Agrobacterium tumefaciens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| beta-glucosidase H0HC94 | Agrobacterium tumefaciens | cellobiose | 233 | 2.94 | 79.32 | pH 7.2, 52 °C | Open record: beta-glucosidase H0HC94, cellobiose |
| beta-glucosidase H0HC94 | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranoside | 277 | 3.09 | 89.94 | pH 7.2, 52 °C | Open record: beta-glucosidase H0HC94, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant C174V | Agrobacterium tumefaciens | cellobiose | 91 | 1.85 | 49.36 | pH 7, 46 °C | Open record: beta-glucosidase H0HC94 variant C174V, cellobiose |
| beta-glucosidase H0HC94variant C174V | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosideMichaelis-Menten non-linear fit; 12 substrate concentrations | 95 | 2.61 | 36.42 | pH 7, 46 °C | Open record: beta-glucosidase H0HC94 variant C174V, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant C174V | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.1 M glucose (product inhibitor) in the assay | 85.7 | 0.44 | 192.8 | pH 7, 46 °C | Open record: beta-glucosidase H0HC94 variant C174V, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant C174V | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.25 M glucose (product inhibitor) in the assay | 91.09 | 0.73 | 123.7 | pH 7, 46 °C | Open record: beta-glucosidase H0HC94 variant C174V, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant C174V | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.5 M glucose (product inhibitor) in the assay | 85.52 | 0.64 | 132.2 | pH 7, 46 °C | Open record: beta-glucosidase H0HC94 variant C174V, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant C174V | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 1 M glucose (product inhibitor) in the assay | 73.42 | 1.25 | 58.5 | pH 7, 46 °C | Open record: beta-glucosidase H0HC94 variant C174V, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant H229S | Agrobacterium tumefaciens | cellobiose | 118 | 5.48 | 21.43 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant H229S, cellobiose |
| beta-glucosidase H0HC94variant H229S | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosideMichaelis-Menten non-linear fit; 12 substrate concentrations | 193 | 2.74 | 70.38 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant H229S, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant H229S | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.1 M glucose (product inhibitor) in the assay | 274.2 | 4.5 | 60.8 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant H229S, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant H229S | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.25 M glucose (product inhibitor) in the assay | 254.0 | 3.29 | 77.08 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant H229S, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant H229S | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.5 M glucose (product inhibitor) in the assay | 240.6 | 4.31 | 55.71 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant H229S, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant H229S | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 1 M glucose (product inhibitor) in the assay | 235.1 | 6.96 | 33.77 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant H229S, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant L178A | Agrobacterium tumefaciens | cellobiose | 328 | 8.25 | 39.73 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant L178A, cellobiose |
| beta-glucosidase H0HC94variant L178A | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosideMichaelis-Menten non-linear fit; 12 substrate concentrations | 390 | 4.99 | 78.16 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant L178A, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant L178A | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.1 M glucose (product inhibitor) in the assay | 312.0 | 4.06 | 76.82 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant L178A, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant L178A | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.25 M glucose (product inhibitor) in the assay | 240.9 | 3.31 | 72.7 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant L178A, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant L178A | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.5 M glucose (product inhibitor) in the assay | 232.1 | 4.93 | 47.01 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant L178A, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant L178A | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 1 M glucose (product inhibitor) in the assay | 167.5 | 1.91 | 87.51 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant L178A, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant L178E | Agrobacterium tumefaciens | cellobiose | 354 | 12.52 | 28.26 | pH 7, 53 °C | Open record: beta-glucosidase H0HC94 variant L178E, cellobiose |
| beta-glucosidase H0HC94variant L178E | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosideMichaelis-Menten non-linear fit; 12 substrate concentrations | 363 | 8.01 | 45.37 | pH 7, 53 °C | Open record: beta-glucosidase H0HC94 variant L178E, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant L178E | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.1 M glucose (product inhibitor) in the assay | 233.2 | 2.65 | 87.78 | pH 7, 53 °C | Open record: beta-glucosidase H0HC94 variant L178E, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant L178E | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.25 M glucose (product inhibitor) in the assay | 200.3 | 2.84 | 70.42 | pH 7, 53 °C | Open record: beta-glucosidase H0HC94 variant L178E, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant L178E | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.5 M glucose (product inhibitor) in the assay | 175.3 | 3.22 | 54.42 | pH 7, 53 °C | Open record: beta-glucosidase H0HC94 variant L178E, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant L178E | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 1 M glucose (product inhibitor) in the assay | 130.5 | 3.68 | 35.42 | pH 7, 53 °C | Open record: beta-glucosidase H0HC94 variant L178E, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant V176A | Agrobacterium tumefaciens | cellobiose | 324 | 22.36 | 14.48 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant V176A, cellobiose |
| beta-glucosidase H0HC94variant V176A | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosideMichaelis-Menten non-linear fit; 12 substrate concentrations | 282 | 6.78 | 41.61 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant V176A, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant V176A | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.1 M glucose (product inhibitor) in the assay | 230.1 | 5.97 | 38.54 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant V176A, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant V176A | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.25 M glucose (product inhibitor) in the assay | 180.1 | 4.68 | 38.42 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant V176A, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant V176A | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.5 M glucose (product inhibitor) in the assay | 140.7 | 3.35 | 41.95 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant V176A, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant V176A | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 1 M glucose (product inhibitor) in the assay | 105.1 | 3.81 | 27.55 | pH 7, 52 °C | Open record: beta-glucosidase H0HC94 variant V176A, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant W127F | Agrobacterium tumefaciens | cellobiose | 107 | 10.14 | 10.5 | pH 6.5, 55 °C | Open record: beta-glucosidase H0HC94 variant W127F, cellobiose |
| beta-glucosidase H0HC94variant W127F | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosideMichaelis-Menten non-linear fit; 12 substrate concentrations | 559 | 19.63 | 28.46 | pH 6.5, 55 °C | Open record: beta-glucosidase H0HC94 variant W127F, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant W127F | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.1 M glucose (product inhibitor) in the assay | 334.9 | 13.31 | 25.16 | pH 6.5, 55 °C | Open record: beta-glucosidase H0HC94 variant W127F, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant W127F | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.25 M glucose (product inhibitor) in the assay | 229.9 | 10.14 | 22.67 | pH 6.5, 55 °C | Open record: beta-glucosidase H0HC94 variant W127F, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant W127F | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 0.5 M glucose (product inhibitor) in the assay | 163.3 | 7 | 23.3 | pH 6.5, 55 °C | Open record: beta-glucosidase H0HC94 variant W127F, p-nitrophenyl-beta-D-glucopyranoside |
| beta-glucosidase H0HC94variant W127F | Agrobacterium tumefaciens | p-nitrophenyl-beta-D-glucopyranosidewith 1 M glucose (product inhibitor) in the assay | 131.5 | 8.48 | 15.5 | pH 6.5, 55 °C | Open record: beta-glucosidase H0HC94 variant W127F, p-nitrophenyl-beta-D-glucopyranoside |