Values CatLog read from this paper

Measurement of incorporation kinetics of non-fluorescent native nucleotides by DNA polymerases using fluorescence microscopy

PMID 29036327doi:10.1093/nar/gkx833Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 8 records (24 values) from this paper: DNA polymerase I Klenow fragment from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.7.7

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
DNA polymerase I Klenow fragment	Escherichia coli	2'-deoxyadenosine 5'-triphosphate	1.69	2.8 × 10⁻⁴	6.04 × 10³	pH 8, 25 °C	Open record: DNA polymerase I Klenow fragment, 2'-deoxyadenosine 5'-triphosphate
DNA polymerase I Klenow fragment	Escherichia coli	2'-deoxycytidine 5'-triphosphate	1.75	2.7 × 10⁻⁴	6.48 × 10³	pH 8, 25 °C	Open record: DNA polymerase I Klenow fragment, 2'-deoxycytidine 5'-triphosphate
DNA polymerase I Klenow fragment	Escherichia coli	2'-deoxyguanosine 5'-triphosphate	6.48	2.2 × 10⁻⁴	2.95 × 10⁴	pH 8, 25 °C	Open record: DNA polymerase I Klenow fragment, 2'-deoxyguanosine 5'-triphosphate
DNA polymerase I Klenow fragment	Escherichia coli	Cy3-dATP	1.16	0.00623	186	pH 8, 25 °C	Open record: DNA polymerase I Klenow fragment, Cy3-dATP
DNA polymerase I Klenow fragment	Escherichia coli	Cy3-dCTP	1.75	0.003	583	pH 8, 25 °C	Open record: DNA polymerase I Klenow fragment, Cy3-dCTP
DNA polymerase I Klenow fragment	Escherichia coli	Cy3-dGTP	1.64	0.0112	146	pH 8, 25 °C	Open record: DNA polymerase I Klenow fragment, Cy3-dGTP
DNA polymerase I Klenow fragment	Escherichia coli	Cy3-dUTP	1.57	0.00893	176	pH 8, 25 °C	Open record: DNA polymerase I Klenow fragment, Cy3-dUTP
DNA polymerase I Klenow fragment	Escherichia coli	thymidine 5'-triphosphate	6.57	3.5 × 10⁻⁴	1.88 × 10⁴	pH 8, 25 °C	Open record: DNA polymerase I Klenow fragment, thymidine 5'-triphosphate