Values CatLog read from this paper
Residues His172 and Lys238 are Essential for the Catalytic Activity of the Maleylacetate Reductase from Sphingobium chlorophenolicum Strain L-1
PMID 29273747doi:10.1038/s41598-017-18475-8Open in Browse
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CatLog read 10 records (30 values) from this paper: maleylacetate reductase (PcpE) from Sphingobium chlorophenolicum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| maleylacetate reductase (PcpE) | Sphingobium chlorophenolicum | 2-chloromaleylacetate | 17.6 | 0.09 | 195.6 | pH 7 | Open record: maleylacetate reductase (PcpE), 2-chloromaleylacetate |
| maleylacetate reductase (PcpE) | Sphingobium chlorophenolicum | NADH250 µM 2-CMA (unpurified preparation, ~70-80% 2-CMA) | 143.7 | 0.13 | 1105 | pH 7 | Open record: maleylacetate reductase (PcpE), NADH |
| maleylacetate reductase (PcpE) | Sphingobium chlorophenolicum | NADH300 µM MA; 3 µg/mL PcpE; NADH or NADPH varied | 1.2 | 0.44 | 2.7 | pH 7 | Open record: maleylacetate reductase (PcpE), NADH |
| maleylacetate reductase (PcpE) | Sphingobium chlorophenolicum | NADPH250 µM 2-CMA (unpurified preparation, ~70-80% 2-CMA) | 29.7 | 0.67 | 44.3 | pH 7 | Open record: maleylacetate reductase (PcpE), NADPH |
| maleylacetate reductase (PcpE) | Sphingobium chlorophenolicum | NADPH300 µM MA; 3 µg/mL PcpE; NADH or NADPH varied | 0.7 | 0.52 | 1.3 | pH 7 | Open record: maleylacetate reductase (PcpE), NADPH |
| maleylacetate reductase (PcpE)variant H236A | Sphingobium chlorophenolicum | maleylacetate | 2.1 | 0.07 | 30 | pH 7 | Open record: maleylacetate reductase (PcpE) variant H236A, maleylacetate |
| maleylacetate reductase (PcpE)variant H237A | Sphingobium chlorophenolicum | maleylacetate | 3.8 | 0.93 | 4.1 | pH 7 | Open record: maleylacetate reductase (PcpE) variant H237A, maleylacetate |
| maleylacetate reductase (PcpE)variant H241A | Sphingobium chlorophenolicum | maleylacetate | 1.7 | 0.16 | 10.6 | pH 7 | Open record: maleylacetate reductase (PcpE) variant H241A, maleylacetate |
| maleylacetate reductase (PcpE)variant H251A | Sphingobium chlorophenolicum | maleylacetate | 8.8 | 1.69 | 5.2 | pH 7 | Open record: maleylacetate reductase (PcpE) variant H251A, maleylacetate |
| maleylacetate reductase (PcpE)variant K140A | Sphingobium chlorophenolicum | maleylacetate | 0.9 | 0.36 | 2.5 | pH 7 | Open record: maleylacetate reductase (PcpE) variant K140A, maleylacetate |