Values CatLog read from this paper
Activity enhancement of CotA laccase by hydrophilic engineering, histidine tag optimization and static culture
PMID 29301022doi:10.1093/protein/gzx064Open in Browse
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CatLog read 7 records (21 values) from this paper: CotA laccase from Bacillus subtilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CotA laccase | Bacillus subtilis | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)N-terminal His6 tag (NH6-CotA) | 26.8 | 0.104 | 260 | Open record: CotA laccase, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | |
| CotA laccase | Bacillus subtilis | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)C-terminal His6 tag (CH6-CotA) | 32.2 | 0.096 | 340 | Open record: CotA laccase, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | |
| CotA laccasevariant F207Y | Bacillus subtilis | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 52.8 | 0.18 | 290 | Open record: CotA laccase variant F207Y, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | |
| CotA laccasevariant F207Y/V403T/P455S | Bacillus subtilis | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 46.4 | 0.054 | 860 | Open record: CotA laccase variant F207Y/V403T/P455S, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | |
| CotA laccasevariant P455S | Bacillus subtilis | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 30 | 0.036 | 830 | Open record: CotA laccase variant P455S, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | |
| CotA laccasevariant V403T | Bacillus subtilis | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 29.2 | 0.06 | 490 | Open record: CotA laccase variant V403T, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | |
| CotA laccasevariant V403T/P455S | Bacillus subtilis | 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 49.8 | 0.09 | 550 | Open record: CotA laccase variant V403T/P455S, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |