Values CatLog read from this paper
A conserved residue of l-alanine dehydrogenase from Bacillus pseudofirmus, Lys-73, participates in the catalytic reaction through hydrogen bonding
PMID 29310857doi:10.1016/j.enzmictec.2017.10.001Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (48 values) from this paper: L-alanine dehydrogenase (OF4Ald) from Bacillus pseudofirmus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| L-alanine dehydrogenase (OF4Ald) | Bacillus pseudofirmus | beta-nicotinamide adenine dinucleotide (NAD+) | 6 | 0.3 | 20 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald), beta-nicotinamide adenine dinucleotide (NAD+) |
| L-alanine dehydrogenase (OF4Ald) | Bacillus pseudofirmus | L-alanine | 10 | 4 | 3 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald), L-alanine |
| L-alanine dehydrogenase (OF4Ald)variant K73A | Bacillus pseudofirmus | beta-nicotinamide adenine dinucleotide (NAD+) | 8 | 0.3 | 24 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald) variant K73A, beta-nicotinamide adenine dinucleotide (NAD+) |
| L-alanine dehydrogenase (OF4Ald)variant K73A | Bacillus pseudofirmus | L-alanine | 18 | 1 | 15 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald) variant K73A, L-alanine |
| L-alanine dehydrogenase (OF4Ald)variant K73E | Bacillus pseudofirmus | beta-nicotinamide adenine dinucleotide (NAD+) | 5 | 0.4 | 11 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald) variant K73E, beta-nicotinamide adenine dinucleotide (NAD+) |
| L-alanine dehydrogenase (OF4Ald)variant K73E | Bacillus pseudofirmus | L-alanine | 3 | 2 | 1 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald) variant K73E, L-alanine |
| L-alanine dehydrogenase (OF4Ald)variant K73F | Bacillus pseudofirmus | beta-nicotinamide adenine dinucleotide (NAD+) | 6 | 0.3 | 19 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald) variant K73F, beta-nicotinamide adenine dinucleotide (NAD+) |
| L-alanine dehydrogenase (OF4Ald)variant K73F | Bacillus pseudofirmus | L-alanine | 4 | 4 | 1 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald) variant K73F, L-alanine |
| L-alanine dehydrogenase (OF4Ald)variant K73Q | Bacillus pseudofirmus | beta-nicotinamide adenine dinucleotide (NAD+) | 2 | 0.2 | 9 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald) variant K73Q, beta-nicotinamide adenine dinucleotide (NAD+) |
| L-alanine dehydrogenase (OF4Ald)variant K73Q | Bacillus pseudofirmus | L-alanine | 2 | 3 | 1 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald) variant K73Q, L-alanine |
| L-alanine dehydrogenase (OF4Ald)variant K73R | Bacillus pseudofirmus | beta-nicotinamide adenine dinucleotide (NAD+) | 7 | 0.4 | 17 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald) variant K73R, beta-nicotinamide adenine dinucleotide (NAD+) |
| L-alanine dehydrogenase (OF4Ald)variant K73R | Bacillus pseudofirmus | L-alanine | 4 | 4 | 1 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald) variant K73R, L-alanine |
| L-alanine dehydrogenase (OF4Ald)variant K73S | Bacillus pseudofirmus | beta-nicotinamide adenine dinucleotide (NAD+) | 7 | 1 | 10 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald) variant K73S, beta-nicotinamide adenine dinucleotide (NAD+) |
| L-alanine dehydrogenase (OF4Ald)variant K73S | Bacillus pseudofirmus | L-alanine | 4 | 4 | 1 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald) variant K73S, L-alanine |
| L-alanine dehydrogenase (OF4Ald)variant K73Y | Bacillus pseudofirmus | beta-nicotinamide adenine dinucleotide (NAD+) | 5 | 0.4 | 14 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald) variant K73Y, beta-nicotinamide adenine dinucleotide (NAD+) |
| L-alanine dehydrogenase (OF4Ald)variant K73Y | Bacillus pseudofirmus | L-alanine | 4 | 3 | 1 | pH 10.5, 40 °C | Open record: L-alanine dehydrogenase (OF4Ald) variant K73Y, L-alanine |