Values CatLog read from this paper
Structural and Mechanistic Analysis of the Choline Sulfatase from Sinorhizobium melliloti: A Class I Sulfatase Specific for an Alkyl Sulfate Ester
PMID 29458126doi:10.1016/j.jmb.2018.02.010Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 34 records (60 values) from this paper: choline sulfatase (SmCS) from Sinorhizobium meliloti.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| choline sulfatase (SmCS) | Sinorhizobium meliloti | 4-nitrophenyl methylphosphonate | 0.1 | 67 | 0.0015 | pH 6, 25 °C | Open record: choline sulfatase (SmCS), 4-nitrophenyl methylphosphonate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | 4-nitrophenyl sulfate | 0.22 | 19 | 0.012 | pH 6, 25 °C | Open record: choline sulfatase (SmCS), 4-nitrophenyl sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | 4-nitrophenyl sulfate25 °C; 4-nitrophenol release followed at 400 nm | 0.34 | 99 | 0.0034 | pH 7.6, 25 °C | Open record: choline sulfatase (SmCS), 4-nitrophenyl sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | 4-nitrophenyl sulfate | — | — | 0.01 | pH 6.4, 25 °C | Open record: choline sulfatase (SmCS), 4-nitrophenyl sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | 4-nitrophenyl sulfate | — | — | 0.0058 | pH 6.8, 25 °C | Open record: choline sulfatase (SmCS), 4-nitrophenyl sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | 4-nitrophenyl sulfate100 mM Imidazole-HCl (pH 7.2) | — | — | 0.0042 | pH 7.2, 25 °C | Open record: choline sulfatase (SmCS), 4-nitrophenyl sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | 4-nitrophenyl sulfate100 mM Tris-HCl (pH 7.2) | — | — | 0.0039 | pH 7.2, 25 °C | Open record: choline sulfatase (SmCS), 4-nitrophenyl sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | 4-nitrophenyl sulfatepH-rate profile; 4-nitrophenol release at 400 nm | — | — | 0.0025 | pH 7.6, 25 °C | Open record: choline sulfatase (SmCS), 4-nitrophenyl sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | 4-nitrophenyl sulfate | — | — | 0.0017 | pH 8, 25 °C | Open record: choline sulfatase (SmCS), 4-nitrophenyl sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | 4-nitrophenyl sulfate | — | — | 7.6 × 10⁻⁴ | pH 8.4, 25 °C | Open record: choline sulfatase (SmCS), 4-nitrophenyl sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | 4-nitrophenyl sulfate100 mM Tris-HCl (pH 8.8) | — | — | 4.5 × 10⁻⁴ | pH 8.8, 25 °C | Open record: choline sulfatase (SmCS), 4-nitrophenyl sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | 4-nitrophenyl sulfate100 mM Glycine-NaOH (pH 8.8) | — | — | 7.9 × 10⁻⁴ | pH 8.8, 25 °C | Open record: choline sulfatase (SmCS), 4-nitrophenyl sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | 4-nitrophenyl sulfate | — | — | 3.3 × 10⁻⁴ | pH 9.2, 25 °C | Open record: choline sulfatase (SmCS), 4-nitrophenyl sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | 4-nitrophenyl sulfate | — | — | 2.4 × 10⁻⁴ | pH 9.6, 25 °C | Open record: choline sulfatase (SmCS), 4-nitrophenyl sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | 4-nitrophenyl sulfate | — | — | 9.2 × 10⁻⁵ | pH 10, 25 °C | Open record: choline sulfatase (SmCS), 4-nitrophenyl sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | choline O-sulfate | 2.4 | 0.5 | 4.8 | pH 7.6, 25 °C | Open record: choline sulfatase (SmCS), choline O-sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | choline-O-sulfate | — | — | 1.3 | pH 6.8, 25 °C | Open record: choline sulfatase (SmCS), choline-O-sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | choline-O-sulfate100 mM Imidazole-HCl (pH 7.2) | — | — | 2.1 | pH 7.2, 25 °C | Open record: choline sulfatase (SmCS), choline-O-sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | choline-O-sulfate100 mM Tris-HCl (pH 7.2) | — | — | 3.6 | pH 7.2, 25 °C | Open record: choline sulfatase (SmCS), choline-O-sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | choline-O-sulfate | — | — | 4.8 | pH 8, 25 °C | Open record: choline sulfatase (SmCS), choline-O-sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | choline-O-sulfate | — | — | 2.7 | pH 8.4, 25 °C | Open record: choline sulfatase (SmCS), choline-O-sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | choline-O-sulfate | — | — | 2.1 | pH 8.8, 25 °C | Open record: choline sulfatase (SmCS), choline-O-sulfate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | methyl 4-nitrophenyl phosphate | — | — | 8 × 10⁻⁵ | pH 6, 25 °C | Open record: choline sulfatase (SmCS), methyl 4-nitrophenyl phosphate |
| choline sulfatase (SmCS) | Sinorhizobium meliloti | phosphocholine | 0.0267 | 2.1 | 0.013 | pH 7.6, 25 °C | Open record: choline sulfatase (SmCS), phosphocholine |
| choline sulfatase (SmCS)variant C54S | Sinorhizobium meliloti | 4-nitrophenyl sulfate | 7.3 × 10⁻⁵ | 39 | 1.89 × 10⁻⁶ | pH 7.6, 25 °C | Open record: choline sulfatase (SmCS) variant C54S, 4-nitrophenyl sulfate |
| choline sulfatase (SmCS)variant C54S | Sinorhizobium meliloti | choline O-sulfate | 0.0112 | 9.4 | 0.00119 | pH 7.6, 25 °C | Open record: choline sulfatase (SmCS) variant C54S, choline O-sulfate |
| choline sulfatase (SmCS)variant E386L | Sinorhizobium meliloti | 4-nitrophenyl sulfate | 0.76 | 57 | 0.013 | pH 7.6, 25 °C | Open record: choline sulfatase (SmCS) variant E386L, 4-nitrophenyl sulfate |
| choline sulfatase (SmCS)variant E386L | Sinorhizobium meliloti | choline O-sulfate | — | — | 8.1 × 10⁻⁴ | pH 7.6, 25 °C | Open record: choline sulfatase (SmCS) variant E386L, choline O-sulfate |
| choline sulfatase (SmCS)variant H104A | Sinorhizobium meliloti | 4-nitrophenyl sulfate | 7.8 × 10⁻⁴ | 202 | 3.8 × 10⁻⁶ | pH 7.6, 25 °C | Open record: choline sulfatase (SmCS) variant H104A, 4-nitrophenyl sulfate |
| choline sulfatase (SmCS)variant H104A | Sinorhizobium meliloti | choline O-sulfate | 0.0347 | 13.3 | 0.0026 | pH 7.6, 25 °C | Open record: choline sulfatase (SmCS) variant H104A, choline O-sulfate |
| choline sulfatase (SmCS)variant K309L | Sinorhizobium meliloti | 4-nitrophenyl sulfate | 1.6 × 10⁻⁵ | 89 | 1.76 × 10⁻⁷ | pH 7.6, 25 °C | Open record: choline sulfatase (SmCS) variant K309L, 4-nitrophenyl sulfate |
| choline sulfatase (SmCS)variant Δ12 | Sinorhizobium meliloti | 4-nitrophenyl sulfate | 0.0159 | 90 | 1.78 × 10⁻⁴ | pH 7.6, 25 °C | Open record: choline sulfatase (SmCS) variant Δ12, 4-nitrophenyl sulfate |
| choline sulfatase (SmCS)variant Δ12 | Sinorhizobium meliloti | choline O-sulfate | 0.021 | 10.9 | 0.00193 | pH 7.6, 25 °C | Open record: choline sulfatase (SmCS) variant Δ12, choline O-sulfate |
| choline sulfatase (SmCS)variant Δ23 | Sinorhizobium meliloti | 4-nitrophenyl sulfate | — | — | 4.77 × 10⁻⁶ | pH 7.6, 25 °C | Open record: choline sulfatase (SmCS) variant Δ23, 4-nitrophenyl sulfate |