Values CatLog read from this paper
Effects of active site residues of 3α-hydroxysteroid dehydrogenase from pseudomonas sp. b-0831 on its catalysis and cofactor binding
PMID 29912635doi:10.1080/09168451.2018.1486175Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (21 values) from this paper: 3α-hydroxysteroid dehydrogenase (Ps3αHSD) from Pseudomonas sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3α-hydroxysteroid dehydrogenase (Ps3αHSD) | Pseudomonas sp. | cholic acidTable 2 | 23 | 0.056 | 410 calc. | pH 8, 37 °C | Open record: 3α-hydroxysteroid dehydrogenase (Ps3αHSD), cholic acid |
| 3α-hydroxysteroid dehydrogenase (Ps3αHSD) | Pseudomonas sp. | cholic acidin the text | — | — | 420 | pH 8, 37 °C | Open record: 3α-hydroxysteroid dehydrogenase (Ps3αHSD), cholic acid |
| 3α-hydroxysteroid dehydrogenase (Ps3αHSD) | Pseudomonas sp. | dehydrocholic acid | 48 | 0.051 | 940 calc. | pH 8, 37 °C | Open record: 3α-hydroxysteroid dehydrogenase (Ps3αHSD), dehydrocholic acid |
| 3α-hydroxysteroid dehydrogenase (Ps3αHSD)variant K157A | Pseudomonas sp. | cholic acidTable 2 | 0.045 | 0.096 | 0.47 calc. | pH 8, 37 °C | Open record: 3α-hydroxysteroid dehydrogenase (Ps3αHSD) variant K157A, cholic acid |
| 3α-hydroxysteroid dehydrogenase (Ps3αHSD)variant K157A | Pseudomonas sp. | cholic acidin the text | — | — | 0.48 | pH 8, 37 °C | Open record: 3α-hydroxysteroid dehydrogenase (Ps3αHSD) variant K157A, cholic acid |
| 3α-hydroxysteroid dehydrogenase (Ps3αHSD)variant S114A | Pseudomonas sp. | cholic acidTable 2 | 0.8 | 0.586 | 1.4 calc. | pH 8, 37 °C | Open record: 3α-hydroxysteroid dehydrogenase (Ps3αHSD) variant S114A, cholic acid |
| 3α-hydroxysteroid dehydrogenase (Ps3αHSD)variant S114A | Pseudomonas sp. | cholic acidin the text | — | — | 1.5 | pH 8, 37 °C | Open record: 3α-hydroxysteroid dehydrogenase (Ps3αHSD) variant S114A, cholic acid |
| 3α-hydroxysteroid dehydrogenase (Ps3αHSD)variant S114A | Pseudomonas sp. | dehydrocholic acid | 0.89 | 0.239 | 3.7 calc. | pH 8, 37 °C | Open record: 3α-hydroxysteroid dehydrogenase (Ps3αHSD) variant S114A, dehydrocholic acid |
| 3α-hydroxysteroid dehydrogenase (Ps3αHSD)variant S114A/Y153F | Pseudomonas sp. | cholic acidTable 2 | 0.012 | 0.078 | 0.15 calc. | pH 8, 37 °C | Open record: 3α-hydroxysteroid dehydrogenase (Ps3αHSD) variant S114A/Y153F, cholic acid |
| 3α-hydroxysteroid dehydrogenase (Ps3αHSD)variant S114A/Y153F | Pseudomonas sp. | cholic acidin the text | — | — | 0.16 | pH 8, 37 °C | Open record: 3α-hydroxysteroid dehydrogenase (Ps3αHSD) variant S114A/Y153F, cholic acid |
| 3α-hydroxysteroid dehydrogenase (Ps3αHSD)variant Y153F | Pseudomonas sp. | cholic acidTable 2 | 0.62 | 0.468 | 1.3 calc. | pH 8, 37 °C | Open record: 3α-hydroxysteroid dehydrogenase (Ps3αHSD) variant Y153F, cholic acid |
| 3α-hydroxysteroid dehydrogenase (Ps3αHSD)variant Y153F | Pseudomonas sp. | cholic acidin the text | — | — | 1.3 | pH 8, 37 °C | Open record: 3α-hydroxysteroid dehydrogenase (Ps3αHSD) variant Y153F, cholic acid |
| 3α-hydroxysteroid dehydrogenase (Ps3αHSD)variant Y153F | Pseudomonas sp. | dehydrocholic acid | 1.1 | 2.489 | 0.44 calc. | pH 8, 37 °C | Open record: 3α-hydroxysteroid dehydrogenase (Ps3αHSD) variant Y153F, dehydrocholic acid |