Values CatLog read from this paper
A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion
PMID 29950589doi:10.1038/s41467-018-04878-2Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (17 values) from this paper: GcoA (cytochrome P450 aryl-O-demethylase, CYP255A family; assayed with reductase GcoB as GcoAB), GcoB (three-domain reductase: 2Fe-2S ferredoxin, FAD and NADH domains) from Amycolatopsis sp. ATCC 39116.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| GcoA (cytochrome P450 aryl-O-demethylase, CYP255A family; assayed with reductase GcoB as GcoAB) | Amycolatopsis sp. ATCC 39116 | 2-ethoxyphenol | 1.4 | 0.015 | 100 | pH 7.5, 25 °C | Open record: GcoA (cytochrome P450 aryl-O-demethylase, CYP255A family; assayed with reductase GcoB as GcoAB), 2-ethoxyphenol |
| GcoA (cytochrome P450 aryl-O-demethylase, CYP255A family; assayed with reductase GcoB as GcoAB) | Amycolatopsis sp. ATCC 39116 | 2-methoxyphenol | 6.8 | 0.06 | 110 | pH 7.5, 25 °C | Open record: GcoA (cytochrome P450 aryl-O-demethylase, CYP255A family; assayed with reductase GcoB as GcoAB), 2-methoxyphenol |
| GcoA (cytochrome P450 aryl-O-demethylase, CYP255A family; assayed with reductase GcoB as GcoAB) | Amycolatopsis sp. ATCC 39116 | 2-methylanisole | 4.6 | 0.027 | 170 | pH 7.5, 25 °C | Open record: GcoA (cytochrome P450 aryl-O-demethylase, CYP255A family; assayed with reductase GcoB as GcoAB), 2-methylanisole |
| GcoA (cytochrome P450 aryl-O-demethylase, CYP255A family; assayed with reductase GcoB as GcoAB) | Amycolatopsis sp. ATCC 39116 | 3-methoxycatechol | 2.1 | 0.03 | 75 | pH 7.5, 25 °C | Open record: GcoA (cytochrome P450 aryl-O-demethylase, CYP255A family; assayed with reductase GcoB as GcoAB), 3-methoxycatechol |
| GcoA (cytochrome P450 aryl-O-demethylase, CYP255A family; assayed with reductase GcoB as GcoAB) | Amycolatopsis sp. ATCC 39116 | Anisole | 3.5 | 0.043 | 82 | pH 7.5, 25 °C | Open record: GcoA (cytochrome P450 aryl-O-demethylase, CYP255A family; assayed with reductase GcoB as GcoAB), Anisole |
| GcoB (three-domain reductase: 2Fe-2S ferredoxin, FAD and NADH domains) | Amycolatopsis sp. ATCC 39116 | NADH | 44 | 0.016 | 2.8 × 10³ calc. | pH 7.5, 25 °C | Open record: GcoB (three-domain reductase: 2Fe-2S ferredoxin, FAD and NADH domains), NADH |