CatLogenzyme kinetics catalog

Values CatLog read from this paper

Single Amino Acid Change in 6-Phosphogluconate Dehydrogenase from Synechocystis Conveys Higher Affinity for NADP+ and Altered Mode of Inhibition by NADPH

PMID 30107441doi:10.1093/pcp/pcy165Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 40 records (48 values) from this paper: 6-phosphogluconate dehydrogenase (Sy6PGDH), 6-phosphogluconate dehydrogenase (Ar6PGDH) from Synechocystis sp. PCC 6803, Arthrospira platensis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.44

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
6-phosphogluconate dehydrogenase (Ar6PGDH)Arthrospira platensisNADP+—0.019—pH 7.8, 43 °COpen
6-phosphogluconate dehydrogenase (Ar6PGDH)Arthrospira platensisNADP+kcat calculated from Vmax16.6——pH 7.8, 43 °COpen
6-phosphogluconate dehydrogenase (Ar6PGDH)Arthrospira platensisNADP+——857pH 7.8, 43 °COpen
6-phosphogluconate dehydrogenase (Ar6PGDH)Arthrospira platensisNADP+in the presence of 0.2 mM NADPH (inhibitor); apparent Km—0.071—pH 7.8, 43 °COpen
6-phosphogluconate dehydrogenase (Ar6PGDH)Arthrospira platensisNADP+in the presence of 0.2 mM NADPH (inhibitor); apparent kcat14.7——pH 7.8, 43 °COpen
6-phosphogluconate dehydrogenase (Ar6PGDH)Arthrospira platensisNADP+in the presence of 0.2 mM NADPH (inhibitor)——212pH 7.8, 43 °COpen
6-phosphogluconate dehydrogenase (Ar6PGDH)variant T35SArthrospira platensisNADP+—0.042—pH 7.8, 43 °COpen
6-phosphogluconate dehydrogenase (Ar6PGDH)variant T35SArthrospira platensisNADP+kcat calculated from Vmax24.2——pH 7.8, 43 °COpen
6-phosphogluconate dehydrogenase (Ar6PGDH)variant T35SArthrospira platensisNADP+——585pH 7.8, 43 °COpen
6-phosphogluconate dehydrogenase (Ar6PGDH)variant T35SArthrospira platensisNADP+in the presence of 0.2 mM NADPH (inhibitor); apparent Km—0.051—pH 7.8, 43 °COpen
6-phosphogluconate dehydrogenase (Ar6PGDH)variant T35SArthrospira platensisNADP+in the presence of 0.2 mM NADPH (inhibitor); apparent kcat13.9——pH 7.8, 43 °COpen
6-phosphogluconate dehydrogenase (Ar6PGDH)variant T35SArthrospira platensisNADP+in the presence of 0.2 mM NADPH (inhibitor)——303pH 7.8, 43 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)Synechocystis sp. PCC 68036-phospho-D-gluconate64.50.0521239pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)Synechocystis sp. PCC 6803NAD+4.894.461.1pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)Synechocystis sp. PCC 6803NADP+—0.058—pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)Synechocystis sp. PCC 6803NADP+kcat calculated from Vmax69.9——pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)Synechocystis sp. PCC 6803NADP+——1207pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)Synechocystis sp. PCC 6803NADP+0.1 mM NADPH; apparent Km—0.056—pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)Synechocystis sp. PCC 6803NADP+0.1 mM NADPH; apparent kcat; kcat calculated from Vmax47.1——pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)Synechocystis sp. PCC 6803NADP+0.1 mM NADPH; apparent kcat/Km——849pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)Synechocystis sp. PCC 6803NADP+0.2 mM NADPH; apparent Km—0.059—pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)Synechocystis sp. PCC 6803NADP+0.2 mM NADPH; apparent kcat; kcat calculated from Vmax20.8——pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)Synechocystis sp. PCC 6803NADP+0.2 mM NADPH; apparent kcat/Km——366pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)Synechocystis sp. PCC 6803NADP+0.2 mM NADH; apparent Km—0.024—pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)Synechocystis sp. PCC 6803NADP+0.2 mM NADH; apparent kcat; kcat calculated from Vmax35.4——pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)Synechocystis sp. PCC 6803NADP+0.2 mM NADH; apparent kcat/Km——1.54 × 10³pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)variant S42TSynechocystis sp. PCC 68036-phospho-D-gluconate510.0421225pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)variant S42TSynechocystis sp. PCC 6803NAD+4.486.60.74pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)variant S42TSynechocystis sp. PCC 6803NADP+—0.015—pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)variant S42TSynechocystis sp. PCC 6803NADP+kcat calculated from Vmax50.6——pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)variant S42TSynechocystis sp. PCC 6803NADP+——3719pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)variant S42TSynechocystis sp. PCC 6803NADP+0.1 mM NADPH; apparent Km—0.043—pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)variant S42TSynechocystis sp. PCC 6803NADP+0.1 mM NADPH; apparent kcat; kcat calculated from Vmax41.2——pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)variant S42TSynechocystis sp. PCC 6803NADP+0.1 mM NADPH; apparent kcat/Km——978pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)variant S42TSynechocystis sp. PCC 6803NADP+0.2 mM NADPH; apparent Km—0.126—pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)variant S42TSynechocystis sp. PCC 6803NADP+0.2 mM NADPH; apparent kcat; kcat calculated from Vmax38.9——pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)variant S42TSynechocystis sp. PCC 6803NADP+0.2 mM NADPH; apparent kcat/Km——306pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)variant S42TSynechocystis sp. PCC 6803NADP+0.2 mM NADH; apparent Km—0.01—pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)variant S42TSynechocystis sp. PCC 6803NADP+0.2 mM NADH; apparent kcat; kcat calculated from Vmax30.4——pH 7.3, 32 °COpen
6-phosphogluconate dehydrogenase (Sy6PGDH)variant S42TSynechocystis sp. PCC 6803NADP+0.2 mM NADH; apparent kcat/Km——3197pH 7.3, 32 °COpen