Values CatLog read from this paper
Structure and Reaction Mechanism of the LigJ Hydratase: An Enzyme Critical for the Bacterial Degradation of Lignin in the Protocatechuate 4,5-Cleavage Pathway
PMID 30207699doi:10.1021/acs.biochem.8b00713Open in Browse
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CatLog read 4 records (12 values) from this paper: LigJ hydratase (KCH hydratase) from Sphingobium sp. SYK-6.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| LigJ hydratase (KCH hydratase) | Sphingobium sp. SYK-6 | (3Z)-2-keto-4-carboxy-3-hexenedioate | 25.6 | 0.0098 | 2.6 × 10³ | pH 8, 30 °C | Open record: LigJ hydratase (KCH hydratase), (3Z)-2-keto-4-carboxy-3-hexenedioate |
| LigJ hydratase (KCH hydratase)variant H223N | Sphingobium sp. SYK-6 | (3Z)-2-keto-4-carboxy-3-hexenedioate | 0.28 | 0.31 | 0.89 | pH 8, 30 °C | Open record: LigJ hydratase (KCH hydratase) variant H223N, (3Z)-2-keto-4-carboxy-3-hexenedioate |
| LigJ hydratase (KCH hydratase)variant T190A | Sphingobium sp. SYK-6 | (3Z)-2-keto-4-carboxy-3-hexenedioate | 10.5 | 0.0119 | 880 | pH 8, 30 °C | Open record: LigJ hydratase (KCH hydratase) variant T190A, (3Z)-2-keto-4-carboxy-3-hexenedioate |
| LigJ hydratase (KCH hydratase)variant Y194F | Sphingobium sp. SYK-6 | (3Z)-2-keto-4-carboxy-3-hexenedioate | 3.3 | 0.0032 | 1 × 10³ | pH 8, 30 °C | Open record: LigJ hydratase (KCH hydratase) variant Y194F, (3Z)-2-keto-4-carboxy-3-hexenedioate |