Values CatLog read from this paper
Improved expression, purification and characterization of VPR, a cold active subtilisin-like serine proteinase and the effects of calcium on expression and stability
PMID 30502512doi:10.1016/j.bbapap.2018.11.010Open in Browse
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CatLog read 5 records (15 values) from this paper: VPR (subtilisin-like serine proteinase) from Vibrio sp. PA-44.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| VPR (subtilisin-like serine proteinase) | Vibrio sp. PA-44 | Succinyl-AlaAlaProPhe-p-nitroanilide | 40.8 | 0.115 | 354 | pH 8.6, 5 °C | Open record: VPR (subtilisin-like serine proteinase), Succinyl-AlaAlaProPhe-p-nitroanilide |
| VPR (subtilisin-like serine proteinase) | Vibrio sp. PA-44 | Succinyl-AlaAlaProPhe-p-nitroanilide | 225.7 | 0.178 | 1239 | pH 8.6, 25 °C | Open record: VPR (subtilisin-like serine proteinase), Succinyl-AlaAlaProPhe-p-nitroanilide |
| VPR (subtilisin-like serine proteinase) | Vibrio sp. PA-44 | Succinyl-AlaAlaProPhe-p-nitroanilide | 552.3 | 0.227 | 2471 | pH 8.6, 45 °C | Open record: VPR (subtilisin-like serine proteinase), Succinyl-AlaAlaProPhe-p-nitroanilide |
| VPR (subtilisin-like serine proteinase) | Vibrio sp. PA-44 | Succinyl-AlaAlaProPhe-p-nitroanilide | 193.1 | 0.198 | 984 | pH 7.4, 25 °C | Open record: VPR (subtilisin-like serine proteinase), Succinyl-AlaAlaProPhe-p-nitroanilide |
| VPR (subtilisin-like serine proteinase) | Vibrio sp. PA-44 | Succinyl-AlaAlaProPhe-p-nitroanilide | 248.7 | 0.133 | 1948 | pH 9.4, 25 °C | Open record: VPR (subtilisin-like serine proteinase), Succinyl-AlaAlaProPhe-p-nitroanilide |