CatLogenzyme kinetics catalog

Values CatLog read from this paper

Archaeal NSUN6 catalyzes m5C72 modification on a wide-range of specific tRNAs

PMID 30541086doi:10.1093/nar/gky1236Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 31 records (89 values) from this paper: PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6 from Pyrococcus horikoshii.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Arg (anticodon GCG), in vitro T7 transcript0.01125.2 × 10⁻⁴21.5pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Asn (anticodon GUU), in vitro T7 transcript0.02937 × 10⁻⁴41.8pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Asp (anticodon GUC), in vitro T7 transcript0.06183 × 10⁻⁴211.2pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Cys (anticodon GCA), in vitro T7 transcriptin the text0.0507——pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Cys (anticodon GCA), in vitro T7 transcriptSupplementary Table S10.05073.8 × 10⁻⁴133.7pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Ser (anticodon CGA), in vitro T7 transcript0.0257.6 × 10⁻⁴32.8pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Ser (anticodon GCU), in vitro T7 transcript0.00625.6 × 10⁻⁴11pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Ser (anticodon GGA), in vitro T7 transcript0.01986.4 × 10⁻⁴31pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Ser (anticodon UGA), in vitro T7 transcript0.03728.3 × 10⁻⁴44.7pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU) C11A:G24U mutant, in vitro T7 transcript0.06953.9 × 10⁻⁴178.2pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU) C11G:G24C mutant, in vitro T7 transcript0.07255.6 × 10⁻⁴129.5pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU) C11G:G24U mutant, in vitro T7 transcript0.06855.7 × 10⁻⁴120.2pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU) C11U mutant, in vitro T7 transcript0.09076.5 × 10⁻⁴139.5pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU) C11U:G24A mutant, in vitro T7 transcript0.07186.7 × 10⁻⁴107.2pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU) C12A:G23U mutant, in vitro T7 transcript0.07175.8 × 10⁻⁴122.5pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU) C12G:G23C mutant, in vitro T7 transcript0.07075 × 10⁻⁴141.3pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU) C12G:G23U mutant, in vitro T7 transcript0.08355.1 × 10⁻⁴164.8pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU) C12U mutant, in vitro T7 transcript0.0695.5 × 10⁻⁴125.5pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU) C12U:G23A mutant, in vitro T7 transcript0.07325.5 × 10⁻⁴133.5pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU) C3A:G70U mutant, in vitro T7 transcript0.1210.00107113.2pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU) C3G:G70C mutant, in vitro T7 transcript0.04759.1 × 10⁻⁴52.2pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU) C3G:G70U mutant, in vitro T7 transcript0.07286.3 × 10⁻⁴115.7pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU) U73G mutant, in vitro T7 transcriptin the text0.03525.9 × 10⁻⁴60 calc.pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU) U73G mutant, in vitro T7 transcriptSupplementary Table S20.03525.9 × 10⁻⁴59.7pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU), in vitro T7 transcriptin the text0.07174.3 × 10⁻⁴170 calc.pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU), in vitro T7 transcriptSupplementary Table S10.07174.3 × 10⁻⁴167pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon GGU), in vitro T7 transcript0.07236.3 × 10⁻⁴110pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6Pyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon UGU), in vitro T7 transcript0.07385.9 × 10⁻⁴125.3pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6variant E174APyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU), in vitro T7 transcript0.06739.5 × 10⁻⁴70.8pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6variant N98APyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU), in vitro T7 transcript0.06859.3 × 10⁻⁴73.7pH 7.5, 65 °COpen
PhNSun6 (PH1991), tRNA:m5C72 methyltransferase NSun6variant Y112APyrococcus horikoshiiPyrococcus horikoshii tRNA-Thr (anticodon CGU), in vitro T7 transcript0.01420.002276.2pH 7.5, 65 °COpen