Values CatLog read from this paper
Mechanism of 6-Hydroxynicotinate 3-Monooxygenase, a Flavin-Dependent Decarboxylative Hydroxylase Involved in Bacterial Nicotinic Acid Degradation
PMID 30810301doi:10.1021/acs.biochem.8b00969Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (32 values) from this paper: 6-hydroxynicotinate 3-monooxygenase (NicC) from Bordetella bronchiseptica.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 6-hydroxynicotinate 3-monooxygenase (NicC) | Bordetella bronchiseptica | 3-chloro-4-hydroxybenzoic acid | — | — | 530 | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC), 3-chloro-4-hydroxybenzoic acid | |
| 6-hydroxynicotinate 3-monooxygenase (NicC) | Bordetella bronchiseptica | 4-hydroxybenzoic acid | 0.074 | 0.6 | 0.12 | pH 7.5, 25 °C | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC), 4-hydroxybenzoic acid |
| 6-hydroxynicotinate 3-monooxygenase (NicC) | Bordetella bronchiseptica | 5-chloro-6-hydroxynicotinic acid | 2.18 | 0.0039 | 560 | pH 7.5, 25 °C | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC), 5-chloro-6-hydroxynicotinic acid |
| 6-hydroxynicotinate 3-monooxygenase (NicC) | Bordetella bronchiseptica | 6-hydroxynicotinic acid | 5 | 0.118 | 42 | pH 7.5, 25 °C | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC), 6-hydroxynicotinic acid |
| 6-hydroxynicotinate 3-monooxygenase (NicC) | Bordetella bronchiseptica | NADH | — | 0.0081 | — | pH 7.5, 25 °C | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC), NADH |
| 6-hydroxynicotinate 3-monooxygenase (NicC)variant C202A | Bordetella bronchiseptica | 6-hydroxynicotinic acid | 0.26 | 0.2 | 1.3 | pH 7.5, 25 °C | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC) variant C202A, 6-hydroxynicotinic acid |
| 6-hydroxynicotinate 3-monooxygenase (NicC)variant C202A | Bordetella bronchiseptica | NADH | — | 0.0063 | — | pH 7.5, 25 °C | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC) variant C202A, NADH |
| 6-hydroxynicotinate 3-monooxygenase (NicC)variant H211A | Bordetella bronchiseptica | 6-hydroxynicotinic acid | 5.9 | 0.193 | 31 | pH 7.5, 25 °C | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC) variant H211A, 6-hydroxynicotinic acid |
| 6-hydroxynicotinate 3-monooxygenase (NicC)variant H211A | Bordetella bronchiseptica | NADH | — | 0.025 | — | pH 7.5, 25 °C | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC) variant H211A, NADH |
| 6-hydroxynicotinate 3-monooxygenase (NicC)variant H302A | Bordetella bronchiseptica | 6-hydroxynicotinic acid | 0.9 | 0.02 | 45 | pH 7.5, 25 °C | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC) variant H302A, 6-hydroxynicotinic acid |
| 6-hydroxynicotinate 3-monooxygenase (NicC)variant H302A | Bordetella bronchiseptica | NADH | — | 0.019 | — | pH 7.5, 25 °C | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC) variant H302A, NADH |
| 6-hydroxynicotinate 3-monooxygenase (NicC)variant H47E | Bordetella bronchiseptica | 6-hydroxynicotinic acid | — | — | 2.3 × 10⁻⁴ | pH 7.5, 25 °C | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC) variant H47E, 6-hydroxynicotinic acid |
| 6-hydroxynicotinate 3-monooxygenase (NicC)variant Y215F | Bordetella bronchiseptica | 6-hydroxynicotinic acid | 12 | 1.6 | 7.5 | pH 7.5, 25 °C | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC) variant Y215F, 6-hydroxynicotinic acid |
| 6-hydroxynicotinate 3-monooxygenase (NicC)variant Y215F | Bordetella bronchiseptica | NADH | — | 0.31 | — | pH 7.5, 25 °C | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC) variant Y215F, NADH |
| 6-hydroxynicotinate 3-monooxygenase (NicC)variant Y225F | Bordetella bronchiseptica | 6-hydroxynicotinic acid | 5.24 | 0.051 | 100 | pH 7.5, 25 °C | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC) variant Y225F, 6-hydroxynicotinic acid |
| 6-hydroxynicotinate 3-monooxygenase (NicC)variant Y225F | Bordetella bronchiseptica | NADH | — | 0.0028 | — | pH 7.5, 25 °C | Open record: 6-hydroxynicotinate 3-monooxygenase (NicC) variant Y225F, NADH |